Mouse Integrin  alpha 2/CD49b Antibody

Catalog # Availability Size / Price Qty
AF1740
AF1740-SP
Detection of Integrin  alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (1)
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Mouse Integrin  alpha 2/CD49b Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 2/CD49b in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately
10% cross‑reactivity with recombinant human Integrin alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin  alpha 5 and rmIntegrin alpha E is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
Tyr27-Thr1129
Accession # Q62469
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  alpha 2/CD49b
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a2/CD49b antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 PE-conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Sheep Anti-Mouse Integrin a2/CD49b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1740) or (B) Normal Sheep IgG Control (Catalog # 5-001-A) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

Immunocytochemistry Integrin a2/CD49b antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Integrin alpha 2/CD49b in Mouse Splenocytes. Integrin a2/CD49b was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Integrin a2/CD49b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2/CD49b

The Integrin family proteins are heterodimeric transmembrane receptors comprised of an alpha and a beta subunit. The Integrin alpha 2 subunit is expressed in monocytes, platelets, B, T and NK cells. It forms a non-covalent heterodimer with the Integrin beta 1 subunit. Integrin alpha 2/ beta 1 binds collagen and laminin and activates intracellular signaling pathways.

Entrez Gene IDs
3673 (Human); 16398 (Mouse)
Alternate Names
alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha

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Citation for Mouse Integrin  alpha 2/CD49b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The small-molecule protein ligand interface stabiliser E7820 induces differential cell line specific responses of integrin &alpha2 expression
    Authors: MD Hülskamp, D Kronenberg, R Stange
    BMC Cancer, 2021-05-18;21(1):571.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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