Mouse Integrin  alpha M/CD11b Alexa Fluor® 750-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse Integrin alpha M/CD11b. Cross-reaction with human Integrin alpha M has been reported (1, 2).
  • Source
    Monoclonal Rat IgG2B Clone # M1/70
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Con A-activated C57BL/10 splenocytes
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 750
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  alpha M/CD11b in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr‑1/Ly‑6G PE‑conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse Integrin  alpha M/CD11b Alexa Fluor® 750‑conjugated Monoclonal Antibody (Catalog # FAB1124S) or (B) Rat IgG2B Alexa Fluor 750 Isotype Control (Catalog # IC013S). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles. The mouse extracellular domain (ECD) shares 87% and 74% amino acid sequence identity with rat and human ECD, respectively (3,4).

  • References:
    1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
    2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
    3. Mitroulis, I. et al. (2015) Pharmacol. Ther. 147:123.
    4. Ross, G.D. et al. (2002) Immunol. Res. 25:219.
  • Entrez Gene IDs:
    3684 (Human); 16409 (Mouse)
  • Alternate Names:
    antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC013S
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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