Mouse Integrin  alpha M/CD11b Alexa Fluor® 750-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1124S-025
Mouse Integrin  alpha M/CD11b Alexa Fluor® 750-conjugated Antibody in Data
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Mouse Integrin  alpha M/CD11b Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha M/CD11b. Cross-reaction with human Integrin alpha M has been reported (1, 2).
Source
Monoclonal Rat IgG2B Clone # M1/70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Con A-activated C57BL/10 splenocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Integrin  alpha M/CD11b in Mouse Bone Marrow Cells by Flow Cytometry. View Larger

Detection of Integrin alpha M/CD11b in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse Integrin aM/CD11b Alexa Fluor® 750-conjugated Monoclonal Antibody (Catalog # FAB1124S) or (B) Rat IgG2BAlexa Fluor 750 Isotype Control (Catalog # IC013S). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles. The mouse extracellular domain (ECD) shares 87% and 74% amino acid sequence identity with rat and human ECD, respectively (3,4).

References
  1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
  2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
  3. Mitroulis, I. et al. (2015) Pharmacol. Ther. 147:123.
  4. Ross, G.D. et al. (2002) Immunol. Res. 25:219.
Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

Product Datasheets

Citation for Mouse Integrin  alpha M/CD11b Alexa Fluor® 750-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. N-Arachidonoyl Dopamine Modulates Acute Systemic Inflammation via Nonhematopoietic TRPV1
    Authors: SK Lawton, F Xu, A Tran, E Wong, A Prakash, M Schumacher, J Hellman, K Wilhelmsen
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse Integrin  alpha M/CD11b Alexa Fluor® 750-conjugated Antibody
By Anonymous on 04/18/2018
Application: Flow Sample Tested: Bone marrow cells Species: Mouse