Mouse Integrin alpha M/CD11b Alexa Fluor® 750-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin alpha M/CD11b in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse Integrin aM/CD11b Alexa Fluor® 750-conjugated Monoclonal Antibody (Catalog # FAB1124S) or (B) Rat IgG2BAlexa Fluor 750 Isotype Control (Catalog # IC013S). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles. The mouse extracellular domain (ECD) shares 87% and 74% amino acid sequence identity with rat and human ECD, respectively (3,4).
- Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
- Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
- Mitroulis, I. et al. (2015) Pharmacol. Ther. 147:123.
- Ross, G.D. et al. (2002) Immunol. Res. 25:219.
Citation for Mouse Integrin alpha M/CD11b Alexa Fluor® 750-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
N-Arachidonoyl Dopamine Modulates Acute Systemic Inflammation via Nonhematopoietic TRPV1
Authors: SK Lawton, F Xu, A Tran, E Wong, A Prakash, M Schumacher, J Hellman, K Wilhelmsen
J. Immunol., 2017;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
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