Mouse CD11b/Integrin alpha M Biotinylated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CD11b/Integrin alpha M in Mouse Spleno-cytes. CD11b/Integrin aM was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin aM Biotinylated Monoclonal Antibody (Catalog # BAM1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.
- Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
- Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Citations for Mouse CD11b/Integrin alpha M Biotinylated Antibody
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Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.
Authors: Schaeffer M, Han SJ, Chtanova T, van Dooren GG, Herzmark P, Chen Y, Roysam B, Striepen B, Robey EA
J. Immunol., 2009;182(10):6379-93.
Sample Types: Whole Tissue
Modulation of macrophage infiltration and inflammatory activity by the phosphatase SHP-1 in virus-induced demyelinating disease.
Authors: Christophi GP, Hudson CA, Panos M, Gruber RC, Massa PT
J. Virol., 2009;83(2):522-39.
Sample Types: Whole Tissue
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