Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # M1/70
View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

Con A-activated C57BL/10 splenocytes

Specificity

Detects mouse CD11b/Integrin alpha M. Cross-reaction with human CD11b/Integrin alpha M has been reported (1, 2).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CD11b/Integrin alpha M Biotinylated Antibody

CD11b/Integrin aM antibody in Mouse Spleno-cytes by Immunocytochemistry (ICC).

CD11b/Integrin alpha M in Mouse Spleno-cytes.

CD11b/Integrin aM was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin aM Biotinylated Monoclonal Antibody (Catalog # BAM1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CD11b/Integrin alpha M in Mouse bone marrow by Flow Cytometry

Mouse bone marrow were stained with Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) APC‑conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rat Anti-Mouse CD11b/Integrin alpha M Biotinylated Monoclonal Antibody (Catalog # BAM1124) or (B) isotype control antibody (Catalog # IC013B) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD11b/Integrin alpha M Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: GR1+ Mouse bone marrow

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunoprecipitation

Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.

Reviewed Applications

Read 1 review rated 5 using BAM1124 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

References

  1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
  2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

Additional CD11b/Integrin alpha M Products

Product Documents for Mouse CD11b/Integrin alpha M Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CD11b/Integrin alpha M Biotinylated Antibody

For research use only

Citations for Mouse CD11b/Integrin alpha M Biotinylated Antibody

Customer Reviews for Mouse CD11b/Integrin alpha M Biotinylated Antibody (1)

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  • Mouse CD11b/Integrin alpha M Biotinylated Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Splenocytes
    Species: Mouse
    Verified Customer | Posted 02/02/2022
    Mouse CD11b/Integrin alpha M Biotinylated Antibody BAM1124

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Protocols

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