Mouse CD11b/Integrin alpha M Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1124
Mouse CD11b/Integrin alpha M Biotinylated Antibody in Data
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Citations (2)
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Mouse CD11b/Integrin alpha M Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD11b/Integrin alpha M. Cross-reaction with human CD11b/Integrin alpha M has been reported (1, 2).
Source
Monoclonal Rat IgG2B Clone # M1/70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Con A-activated C57BL/10 splenocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
Immunohistochemistry
8-25 µg/mL
Immunoprecipitation
Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry CD11b/Integrin  alpha M in Mouse Spleno-cytes. View Larger

CD11b/Integrin alpha M in Mouse Spleno-cytes. CD11b/Integrin aM was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin aM Biotinylated Monoclonal Antibody (Catalog # BAM1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

References
  1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
  2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

Product Datasheets

Citations for Mouse CD11b/Integrin alpha M Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.
    Authors: Schaeffer M, Han SJ, Chtanova T, van Dooren GG, Herzmark P, Chen Y, Roysam B, Striepen B, Robey EA
    J. Immunol., 2009;182(10):6379-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Modulation of macrophage infiltration and inflammatory activity by the phosphatase SHP-1 in virus-induced demyelinating disease.
    Authors: Christophi GP, Hudson CA, Panos M, Gruber RC, Massa PT
    J. Virol., 2009;83(2):522-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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