Mouse CD11b/Integrin alpha M Antibody

Catalog # Availability Size / Price Qty
MAB1124-SP
MAB1124-500
MAB1124-100
Mouse CD11b/Integrin alpha M Antibody in Data
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Citations (15)
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Mouse CD11b/Integrin alpha M Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD11b/Integrin alpha M. Cross-reaction with human CD11b/Integrin alpha M has been reported (1, 2).
Source
Monoclonal Rat IgG2B Clone # M1/70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Con A-activated C57BL/10 splenocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
Immunohistochemistry
Hata, H., et al. (2004) J. Clin. Invest. 114:582.
Iwasaki, A. and Kelsall, B.L. (2002) J. Immunol. 166:4884.
Zhang, Y., et al. (2002) J. Immunol. 168:3088.
 
Immunoprecipitation
Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
 
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry CD11b/Integrin  alpha M in Mouse Splenocytes. View Larger

CD11b/Integrin alpha M in Mouse Splenocytes. CD11b/Integrin aM was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin aM Monoclonal Antibody (Catalog # MAB1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

References
  1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
  2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

Product Datasheets

Citations for Mouse CD11b/Integrin alpha M Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Angiostrongylus cantonensis Galectin-1 interacts with Annexin A2 to impair the viability of macrophages via activating JNK pathway
    Authors: X Shi, M Xiao, Z Xie, Q Shi, Y Zhang, JW Leavenwort, B Yan, H Huang
    Parasit Vectors, 2020;13(1):183.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. PU.1 controls fibroblast polarization and tissue fibrosis
    Authors: T Wohlfahrt, S Rauber, S Uebe, M Luber, A Soare, A Ekici, S Weber, AE Matei, CW Chen, C Maier, E Karouzakis, HP Kiener, E Pachera, C Dees, C Beyer, C Daniel, K Gelse, AE Kremer, E Naschberge, M Stürzl, F Butter, M Sticherlin, S Finotto, A Kreuter, MH Kaplan, A Jüngel, S Gay, SL Nutt, DW Boykin, GMK Poon, O Distler, G Schett, JHW Distler, A Ramming
    Nature, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Human IgM antibody rHIgM22 promotes phagocytic clearance of myelin debris by microglia
    Authors: Y Zorina, J Stricker, AO Caggiano, DC Button
    Sci Rep, 2018;8(1):9392.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Chi3l3: a potential key orchestrator of eosinophil recruitment in meningitis induced by Angiostrongylus cantonensis
    Authors: S Wan, X Sun, F Wu, Z Yu, L Wang, D Lin, Z Li, Z Wu, X Sun
    J Neuroinflammation, 2018;15(1):31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Group 2 Innate Lymphoid Cells Attenuate Inflammatory Arthritis and Protect from Bone Destruction in Mice
    Authors: Y Omata, M Frech, T Primbs, S Lucas, D Andreev, C Scholtysek, K Sarter, M Kindermann, N Yeremenko, DL Baeten, N Andreas, T Kamradt, A Bozec, A Ramming, G Krönke, S Wirtz, G Schett, MM Zaiss
    Cell Rep, 2018;24(1):169-180.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease
    Authors: SR Stott, S Hayat, T Carnwath, S Garas, JP Sleeman, RA Barker
    PLoS ONE, 2017;12(2):e0171748.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
    Authors: Clara Jana-Lui Busch
    Hepatology, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
    Sci Rep, 2016;6(0):31874.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
    Authors: Zhang, Hanwei, Chin, Arnold I
    PLoS ONE, 2014;9(4):e94793.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Absence of T and B lymphocytes modulates dystrophic features in dysferlin deficient animal model.
    Authors: Farini A, Sitzia C, Navarro C, D'Antona G, Belicchi M, Parolini D, Del Fraro G, Razini P, Bottinelli R, Meregalli M, Torrente Y
    Exp. Cell Res., 2012;318(10):1160-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
    J Neuroinflammation, 2012;9(0):193.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Sialoadhesin expression in intact degenerating retinas and following transplantation.
    Authors: Sancho-Pelluz J, Wunderlich KA, Rauch U, Romero FJ, van Veen T, Limb GA, Crocker PR, Perez MT
    Invest. Ophthalmol. Vis. Sci., 2008;49(12):5602-10.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Inhibition of experimental autoimmune encephalomyelitis by a novel small molecular weight proinflammatory cytokine suppressing drug.
    Authors: Karpus WJ, Reynolds N, Behanna HA, Van Eldik LJ, Watterson DM
    J. Neuroimmunol., 2008;203(1):73-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. LDL-receptor-related protein regulates beta2-integrin-mediated leukocyte adhesion.
    Authors: Spijkers PP, da Costa Martins P, Westein E, Gahmberg CG, Zwaginga JJ, Lenting PJ
    Blood, 2005;105(1):170-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  15. Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice.
    Authors: Tezel G, Yang X, Yang J, Wax MB
    Brain Res., 2004;996(2):202-12.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Cell and Tissue Staining Kits

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Isotype Controls

Rat IgG2B Isotype Control

Reconstitution Buffers

Secondary Antibodies

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