Mouse CD11b/Integrin alpha M Antibody

(9 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD11b/Integrin alpha M. Cross-reaction with human CD11b/Integrin alpha M has been reported (1, 2).
  • Source
    Monoclonal Rat IgG2B Clone # M1/70
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Con A-activated C57BL/10 splenocytes
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse splenocytes
  • Immunohistochemistry
    Hata, H., et al. (2004) J. Clin. Invest. 114:582.
    Iwasaki, A. and Kelsall, B.L. (2002) J. Immunol. 166:4884.
    Zhang, Y., et al. (2002) J. Immunol. 168:3088.
  • Immunoprecipitation
    Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
  • CyTOF-reported
    Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD11b/Integrin alpha M in Mouse Splenocytes.
CD11b/Integrin alpha M was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

  • References:
    1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
    2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
  • Entrez Gene IDs:
    3684 (Human); 16409 (Mouse)
  • Alternate Names:
    antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease
    Authors: SR Stott, S Hayat, T Carnwath, S Garas, JP Sleeman, RA Barker
    PLoS ONE, 2017;12(2):e0171748.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
    Sci Rep, 2016;6(0):31874.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
    Authors: Zhang, Hanwei, Chin, Arnold I
    PLoS ONE, 2014;9(4):e94793.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Absence of T and B lymphocytes modulates dystrophic features in dysferlin deficient animal model.
    Authors: Farini A, Sitzia C, Navarro C, D'Antona G, Belicchi M, Parolini D, Del Fraro G, Razini P, Bottinelli R, Meregalli M, Torrente Y
    Exp. Cell Res., 2012;318(10):1160-74.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
    J Neuroinflammation, 2012;9(0):193.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Inhibition of experimental autoimmune encephalomyelitis by a novel small molecular weight proinflammatory cytokine suppressing drug.
    Authors: Karpus WJ, Reynolds N, Behanna HA, Van Eldik LJ, Watterson DM
    J. Neuroimmunol., 2008;203(1):73-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Sialoadhesin expression in intact degenerating retinas and following transplantation.
    Authors: Sancho-Pelluz J, Wunderlich KA, Rauch U, Romero FJ, van Veen T, Limb GA, Crocker PR, Perez MT
    Invest. Ophthalmol. Vis. Sci., 2008;49(12):5602-10.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. LDL-receptor-related protein regulates beta2-integrin-mediated leukocyte adhesion.
    Authors: Spijkers PP, da Costa Martins P, Westein E, Gahmberg CG, Zwaginga JJ, Lenting PJ
    Blood, 2005;105(1):170-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  9. Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice.
    Authors: Tezel G, Yang X, Yang J, Wax MB
    Brain Res., 2004;996(2):202-12.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
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