Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 268622
View all Integrin beta 6 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 6
Gly22-Asn706
Accession # Q9Z0T9

Specificity

Detects mouse Integrin beta 6 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Integrin  beta 6 Antibody

Integrin beta 6 antibody in XB2 Mouse Cell Line by Immunocytochemistry (ICC).

Integrin beta 6 in XB2 Mouse Cell Line.

Integrin beta 6 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Rat Anti-Mouse Integrin beta 6 Monoclonal Antibody (Catalog # MAB2389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Integrin  beta 6 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed XB2 mouse teratoma keratinocyte cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Integrin  beta 6 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin beta 6

Integrin beta 6 is a type I transmembrane Integrin beta subunit that heterodimerizes with alpha V subunits in the epithelial restricted integrin alpha V/ beta 6. The extracellular domain of mouse Integrin beta 6 contains a collagen binding von-Willebrand factor A domain and multiple truncated EGF-like motifs. Mouse Integrin beta 6 extracellular domain shares 96% and 91% amino acid sequence identity with the corresponding regions of the rat and human protein, respectively.

Alternate Names

ITGB6

Entrez Gene IDs

3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)

Gene Symbol

ITGB6

UniProt

Q9Z0T9

Additional Integrin beta 6 Products

Product Documents for Mouse Integrin  beta 6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Integrin  beta 6 Antibody

For research use only

Citations for Mouse Integrin  beta 6 Antibody

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Protocols

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