Detects mouse Kallikrein 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Kallikrein 1, recombinant mouse (rm) Kallikrein 5 and rmKallikrein 7 is observed.
Detection of Mouse Kallikrein 1 by Western Blot. Western blot shows lysates of mouse pancreas tissue and mouse small intestine. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse Kallikrein 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7928) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kallikrein 1 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kallikrein 1 in Mouse Kidney. Kallikrein 1 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Kallikrein 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7928) at 1.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 1
The kallikreins are a family of trypsin‑like serine proteases, many of which are associated with a variety of cancers. Kallikrein 1 (KLK1) also known as tissue kallikrein and urinary kallikrein is a 25 - 30 kDa member of peptidase S1 family of proteins. At least two isoforms are predicted. An important physiological function of KLK1 is the cleavage of kininogen to release a vasoactive kinin peptide, bradykinin (for rodent KLK1) or lysyl‑bradykinin (for human KLK1). Kinins regulate vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation. Over aa 25-261, mouse KLK1 shares 64% aa identity with human KLK1.
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