Mouse Kallikrein 1 Antibody

Catalog # Availability Size / Price Qty
AF7928
AF7928-SP

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Detection of Mouse Kallikrein 1 by Western Blot.
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Mouse Kallikrein 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Kallikrein 1 in direct ELISAs and Western blots.  In direct ELISAs, less than 1% cross-reactivity with recombinant human Kallikrein 1, recombinant mouse (rm) Kallikrein 5 and rmKallikrein 7 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein 5 precursor/Kallikrein 1
Val23-Arg67(Kallikrein 5 precursor), Ile25-Asp261(Kallikrein 1)
Accession # NP_081082, P15947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Kallikrein 1 antibody by Western Blot. View Larger

Detection of Mouse Kallikrein 1 by Western Blot. Western blot shows lysates of mouse pancreas tissue and mouse small intestine. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse Kallikrein 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7928) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kallikrein 1 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Kallikrein 1 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Kallikrein 1 in Mouse Kidney. Kallikrein 1 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Kallikrein 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7928) at 1.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 1

The kallikreins are a family of trypsin‑like serine proteases, many of which are associated with a variety of cancers. Kallikrein 1 (KLK1) also known as tissue kallikrein and urinary kallikrein is a 25 - 30 kDa member of peptidase S1 family of proteins. At least two isoforms are predicted. An important physiological function of KLK1 is the cleavage of kininogen to release a vasoactive kinin peptide, bradykinin (for rodent KLK1) or lysyl‑bradykinin (for human KLK1). Kinins regulate vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation.  Over aa 25-261, mouse KLK1 shares 64% aa identity with human KLK1.

Entrez Gene IDs
3816 (Human); 16612 (Mouse)
Alternate Names
EC 3.4.21; EC 3.4.21.35; glandular kallikrein 1; hK1; Kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein serine protease 1; kallikrein-1; Kidney/pancreas/salivary gland kallikrein; KLK1; Klk6; KLKR; Tissue kallikrein

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