Killer cell lectin-like receptor family E member 1 (Klre-1), also called NKG2I (NKG2 family member I), is a 24-28 kDa member of the NKG2/KLR family of proteins. It is expressed on mouse NK and NKT cells, and appears to serve as one component of two novel heterodimeric cell surface receptors. When complexed to KLRI1, Klre-1 inhibits NK cell cytotoxic activity. When complexed to KLRI2, Klre-1 activates NK cells, inducing IFN-gamma production and the activation of a cytolytic program. Although Klre-1 preferentially associates noncovalently with NLRI1 and I2, it apparently will form disulfide-linked homodimers in the absence of its heterodimer partners. Mouse Klre-1 is a 226 amino acid (aa) type II transmembrane protein. It contains an N-terminal cytoplasmic segment (aa 1-68) plus a 133 aa extracellular region (aa 94-226) that possesses one C-type lectin domain (aa 110-226). Over aa 94-226, mouse Klre-1 shares 82% aa identity with rat Klre-1. There does not appear to be a human structural ortholog to mouse Klre-1.
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 854929
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Product Specifications
Immunogen
Chinese hamster ovary cell line, CHO derived recombinant mouse Klre-1
Lys94-Lys226
Accession # NP_705818
Lys94-Lys226
Accession # NP_705818
Specificity
Detects mouse Klre-1 in ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Klre‑1 Antibody
Detection of Klre‑1 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse Klre-1 Monoclonal Antibody (Catalog # MAB7099) or (B) Rat IgG2BIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).Applications for Mouse Klre‑1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Klre-1
Long Name
Killer Cell Lectin-like Receptor Family E Member 1
Alternate Names
Klre1, NKG2I
Entrez Gene IDs
243655 (Mouse)
Gene Symbol
KLRE1
UniProt
Additional Klre-1 Products
Product Documents for Mouse Klre‑1 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Klre‑1 Antibody
For research use only
Related Research Areas
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