Mouse Klre-1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Klre-1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human NKG2D and recombinant mouse NKG2D is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Klre-1
    Lys94-Lys226
    Accession # NP_705818
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Klre‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse Klre‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7099) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Anti-Mouse NK1.1 PE-conjugated antibody. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Klre-1

NKG2I (NKG2 family member I; also Klre-1) is a 24-28 kDa member of the NKG2/KLR family of proteins. It is expressed on mouse NK and NKT cells, and appears to serve as one component of two novel heterodimeric cell surface receptors. When complexed to KLRI1, NKG2I inhibits NK cell cytotoxic activity. When complexed to KLRI2, NKG2I activates NK cells, inducing IFN-gamma production and the activation of a cytolytic program. Although NKG2I preferentially associates noncovalently with NLRI1 and I2, it apparently will form disulfide-linked homodimers in the absence of its heterodimer partners. Mouse NKG2I is a 226 amino acid (aa) type II transmembrane protein. It contains an N-terminal cytoplasmic segment (aa 1-68) plus a 133 aa extracellular region (aa 94-226) that possesses one C-type lectin domain (aa 110-226). Over aa 94-226, mouse NKG2I shares 82% aa identity with rat NKG2I. There does not appear to be a human structural ortholog to mouse NKG2I.

  • Long Name:
    Killer Cell Lectin-like Receptor Family E Member 1
  • Entrez Gene IDs:
    243655 (Mouse)
  • Alternate Names:
    Klre1; Klre-1; NKG2I
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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