Detection of Klre‑1 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Sheep Anti-Mouse Klre‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7099) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Anti-Mouse NK1.1 PE-conjugated antibody. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
NKG2I (NKG2 family member I; also Klre-1) is a 24-28 kDa member of the NKG2/KLR family of proteins. It is expressed on mouse NK and NKT cells, and appears to serve as one component of two novel heterodimeric cell surface receptors. When complexed to KLRI1, NKG2I inhibits NK cell cytotoxic activity. When complexed to KLRI2, NKG2I activates NK cells, inducing IFN-gamma production and the activation of a cytolytic program. Although NKG2I preferentially associates noncovalently with NLRI1 and I2, it apparently will form disulfide-linked homodimers in the absence of its heterodimer partners. Mouse NKG2I is a 226 amino acid (aa) type II transmembrane protein. It contains an N-terminal cytoplasmic segment (aa 1-68) plus a 133 aa extracellular region (aa 94-226) that possesses one C-type lectin domain (aa 110-226). Over aa 94-226, mouse NKG2I shares 82% aa identity with rat NKG2I. There does not appear to be a human structural ortholog to mouse NKG2I.
Killer Cell Lectin-like Receptor Family E Member 1
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