Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 771312
View all Kynureninase Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Kynureninase
Met1-Ser464
Accession # Q9CXF0

Specificity

Detects mouse Kynureninase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Kynureninase is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Kynureninase Antibody

Detection of Mouse Kynureninase antibody by Western Blot.

Detection of Mouse Kynureninase by Western Blot.

Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Kynureninase Monoclonal Antibody (Catalog # MAB7389) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kynureninase antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

Kynureninase in Mouse Liver.

Kynureninase was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse Kynureninase Monoclonal Antibody (Catalog # MAB7389) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Kynureninase Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

0.5 µg/mL
Sample: Mouse liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kynureninase

Kyureninase (KYNU, a hydrolase that acts on dog [Greek: kyon] urine [Greek: ouron]) is a 50-55 kDa member of the kyureninase family of enzymes.  It is a pyridoxal phosphate-dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages.  Kyureninase participates in the metabolism of tryptophan.  Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD.  Kyureninase catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3-hydroxyanthranilic acid.  Mouse Kyureninase is 464 amino acids (aa) in length.  It contains one large catalytic site (aa 24-462), and typically functions as a noncovalent homodimer.  There are at least two potential isoform variants.  One contains a three aa substitution for aa 425-464, while another possesses a 22 aa substitution for aa 302-464.  Full-length mouse Kyureninase shares 92% and 83% aa sequence identity with rat and human Kyureninase, respectively.    

Alternate Names

KYNU, L-kynurenine hydrolase

Entrez Gene IDs

8942 (Human); 70789 (Mouse); 116682 (Rat)

Gene Symbol

KYNU

UniProt

Q9CXF0

Additional Kynureninase Products

Product Documents for Mouse Kynureninase Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Kynureninase Antibody

For research use only

Related Research Areas

Kynurenine Pathway

Citations for Mouse Kynureninase Antibody

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Protocols

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FAQs

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