Detection of Mouse Kynureninase by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Kynureninase Monoclonal Antibody (Catalog # MAB7389) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kynureninase in Mouse Liver. Kynureninase was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse Kynureninase Monoclonal Antibody (Catalog # MAB7389) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Kyureninase (KYNU, a hydrolase that acts on dog [Greek: kyon] urine [Greek: ouron]) is a 50-55 kDa member of the kyureninase family of enzymes. It is a pyridoxal phosphate-dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages. Kyureninase participates in the metabolism of tryptophan. Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD. Kyureninase catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3-hydroxyanthranilic acid. Mouse Kyureninase is 464 amino acids (aa) in length. It contains one large catalytic site (aa 24-462), and typically functions as a noncovalent homodimer. There are at least two potential isoform variants. One contains a three aa substitution for aa 425-464, while another possesses a 22 aa substitution for aa 302-464. Full-length mouse Kyureninase shares 92% and 83% aa sequence identity with rat and human Kyureninase, respectively.
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