Mouse Kynureninase Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Kynureninase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Kynureninase is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 771312
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Kynureninase
    Met1-Ser464
    Accession # Q9CXF0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Kynureninase by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Kynureninase Monoclonal Antibody (Catalog # MAB7389) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Kynureninase in Mouse Liver. Kynureninase was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse Kynureninase Monoclonal Antibody (Catalog # MAB7389) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kynureninase
Kyureninase (KYNU, a hydrolase that acts on dog [Greek: kyon] urine [Greek: ouron]) is a 50-55 kDa member of the kyureninase family of enzymes.  It is a pyridoxal phosphate-dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages.  Kyureninase participates in the metabolism of tryptophan.  Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD.  Kyureninase catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3-hydroxyanthranilic acid.  Mouse Kyureninase is 464 amino acids (aa) in length.  It contains one large catalytic site (aa 24-462), and typically functions as a noncovalent homodimer.  There are at least two potential isoform variants.  One contains a three aa substitution for aa 425-464, while another possesses a 22 aa substitution for aa 302-464.  Full-length mouse Kyureninase shares 92% and 83% aa sequence identity with rat and human Kyureninase, respectively.    
  • Entrez Gene IDs:
    8942 (Human); 70789 (Mouse); 116682 (Rat)
  • Alternate Names:
    EC 3.7.1.3; KYNU; kynureninase (L-kynurenine hydrolase); Kynureninase; L-kynurenine hydrolase
Related Research Areas
Cell and Tissue Staining Kits
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Anti-Rat HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

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VisUCyte Antigen Retrieval Reagent-Acidic

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Isotype Controls
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Rat IgG2B Isotype Control

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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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