Detects mouse LAG-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human LAG-3 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant mouse LAG-3
Accession # Q61790
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols
are available in the Technical Information section on our website.
Detection of LAG‑3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Goat Anti-Mouse LAG‑3 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3328P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
LAG-3 (Lymphocyte Activation Gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity. The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.
Lymphocyte-activation Gene 3
Entrez Gene IDs:
3902 (Human); 16768 (Mouse); 297596 (Rat)
CD223; CD223; CD223 antigen; FDC; LAG-3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC
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