Mouse Laminin-1 Antibody

Catalog # Availability Size / Price Qty
MAB2549
MAB2549-SP
Laminin‑1 in Mouse Embryo.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Mouse Laminin-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with mouse Laminin-1 A and B chains on the long arm below the cross region.
Source
Monoclonal Rat IgG1 Clone # AL-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot

Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems.

 
Immunohistochemistry
8-25 µg/mL
See below
Adhesion Blockade
Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Laminin-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Laminin‑1 in Mouse Embryo. Laminin-1 was detected in immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5) using Mouse Laminin-1 Monoclonal Antibody (Catalog # MAB2549) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 637-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin-1

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival.

Alternate Names
LAMA; LAMA1; laminin subunit alpha 1; Laminin1; Laminin-1; Laminin-111; PTBHS; S-LAM-alpha

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Citation for Mouse Laminin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Chlorination and oxidation of the extracellular matrix protein laminin and basement membrane extracts by hypochlorous acid and myeloperoxidase
    Authors: T Nybo, S Dieterich, LF Gamon, CY Chuang, A Hammer, G Hoefler, E Malle, A Rogowska-W, MJ Davies
    Redox Biol, 2018-11-03;20(0):496-513.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot

FAQs

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Reviews for Mouse Laminin-1 Antibody

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Mouse Laminin-1 Antibody
By Anonymous on 10/31/2021
Application: WB Sample Tested: Lung tissue Species: Mouse

Mouse Laminin-1 Antibody
By Anonymous on 07/17/2021
Application: WB Sample Tested: Brain tissue Species: Mouse