Mouse LAMP1/CD107a Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LAMP1/CD107a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAMP1/CD107a is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 747203
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse LAMP1/CD107a
    Leu25-Asn370
    Accession # P11438
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
LAMP1/CD107a in RAW 264.7 Mouse Cell Line. LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse LAMP1/CD107a Monoclonal Antibody (Catalog # MAB4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAMP-1/CD107a
LAMP1 (lysosome-associated membrane glycoprotein 1; also P2B and CD107a) is a 120 kDa member of the LAMP family of proteins. It is a highly glycosylated type I transmembrane protein whose N-terminus projects either extracellularly or into the lumen of lysosomes. LAMP1 may protect lysosomal membranes from enzymatic attack or mediate fusion of phagosomes with lysosomes. Mature mouse LAMP1 is 382 amino acids (aa) in length. It contains a 346 aa luminal domain (aa 25‑370) and a 12 aa cytoplasmic region. Over aa 25‑370, mouse LAMP1 shares 82% and 66% aa identity with rat and human LAMP1, respectively. 
  • Long Name:
    Lysosome-associated Membrane Glycoprotein 1
  • Entrez Gene IDs:
    3916 (Human); 16783 (Mouse)
  • Alternate Names:
    CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Sphingosine regulates the NLRP3-inflammasome and IL-1beta release from macrophages.
    Authors: Luheshi NM, Giles JA, Lopez-Castejon G, Brough D
    Eur. J. Immunol., 2011;42(3):716-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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Anti-Rat HRP-DAB Cell & Tissue Staining Kit

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Isotype Controls
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Rat IgG2A Isotype Control

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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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