Mouse LAP (TGF-beta 1) Antibody

Catalog # Availability Size / Price Qty
MAB7666
MAB7666-SP
Mouse LAP (TGF-beta 1) Antibody in Flow Cytometry
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Mouse LAP (TGF-beta 1) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LAP (TGF-beta 1) in flow cytometry.
Source
Monoclonal Rat IgG2A Clone # 860206
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse LAP (TGF-beta 1)
Met1-Ser390
Accession # P04202
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of LAP (TGF‑ beta 1) in Mouse iTreg cells by Flow Cytometry. View Larger

Detection of LAP (TGF‑ beta 1) in Mouse iTreg cells by Flow Cytometry. Mouse splenocytes treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 μg/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 μg/mL Recombinant Mouse IL‑2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF‑ beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

TGF-beta 1 (Transforming Growth Factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa Latency‑Associated Peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI, also known as Activin Receptor‑Like Kinase 5(ALK‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors Betaglycan (also known as TGF‑ beta  RIII) and Endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

References
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name
Latency-associated Peptide
Entrez Gene IDs
7040 (Human)
Alternate Names
CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

Product Datasheets

Citations for Mouse LAP (TGF-beta 1) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient
    Authors: J Beumer, B Artegiani, Y Post, F Reimann, F Gribble, TN Nguyen, H Zeng, M Van den Bo, JH Van Es, H Clevers
    Nat. Cell Biol., 2018;20(8):909-916.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  2. AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage
    Authors: CC Fang, CF Wu, YJ Liao, SF Huang, M Chen, YA Chen
    Sci Rep, 2018;8(1):13802.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Suppressive IL-17A(+)Foxp3(+) and ex-Th17 IL-17A(neg)Foxp3(+) Treg cells are a source of tumour-associated Treg cells
    Authors: S Downs-Cann, S Berkey, GM Delgoffe, RP Edwards, T Curiel, K Odunsi, DL Bartlett, N Obermajer
    Nat Commun, 2017;8(0):14649.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Culture supernatant of adipose stem cells can ameliorate allergic airway inflammation via recruitment of CD4(+)CD25(+)Foxp3 T cells
    Authors: HS Yu, MK Park, SA Kang, KS Cho, SJ Mun, HJ Roh
    Stem Cell Res Ther, 2017;8(1):8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis
    Authors: F Wirsdörfer, S de Leve, F Cappuccini, T Eldh, AV Meyer, E Gau, LF Thompson, NY Chen, H Karmouty-Q, U Fischer, M Kasper, D Klein, JW Ritchey, MR Blackburn, AM Westendorf, M Stuschke, V Jendrossek
    Cancer Res., 2016;76(10):3045-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages.
    Authors: Lee Y, Park H, Woo S, Park E, Kang J
    J Pharmacol Exp Ther, 2014;350(3):563-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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Mouse LAP (TGF-beta 1) Antibody
By Anonymous on 03/29/2019
Application: ELISA Sample Tested: Recombinant protein Species: Mouse

confirmed antibody specificity using recombinant proteins in a direct ELISA