Mouse Latent TGF-beta  bp4 Antibody

Catalog # Availability Size / Price Qty
AF2885
AF2885-SP
Product Details
Citations (5)
FAQs
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Mouse Latent TGF-beta  bp4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Latent TGF‑ beta bp4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) LTBP-1 and rhLTBP-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Latent TGF‑ beta bp4 isoform 1
Ser25-Ala1666 (Arg542-Pro547)
Accession # AAI41006
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Latent TGF-beta  bp4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latent TGF-beta bp4

LTBP-4 is a 170 kDa extracellular glycoprotein that belongs to the LTBP/fibrillin family. These proteins contain a conserved pattern of eight cysteine residues (8Cys) and EGF-like domains. Mouse LTBP-4 isoform 1 is synthesized as 1666 amino acid residues and contains 14 EGF-like motifs and three 8Cys, the 3rd of which is responsible for disulfide-association with the latency-linked associated peptide (LAP) of latent TGF-beta 1. LTBP-4 is expressed in multiple tissues and is involved in the assembly, secretion and targeting of latent TGF-beta to sites where it can be activated. Two additional splice isoforms that differ from isoform 1 in residues 60‑150 or 67‑150, have been described. Human and mouse LTBP-4 share 90% amino acid sequence homology.

Entrez Gene IDs
8425 (Human)
Alternate Names
Latent TGFbeta bp4; Latent TGF-beta bp4; latent transforming growth factor beta binding protein 4; LTBP4; LTBP-4; LTBP4L; LTBP4S

Product Datasheets

Citations for Mouse Latent TGF-beta  bp4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Fibulin-4 deposition requires EMILIN-1 in the extracellular matrix of osteoblasts
    Authors: A Schiavinat, DR Keene, T Imhof, R Doliana, T Sasaki, G Sengle
    Sci Rep, 2017;7(1):5526.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Latent TGF-? binding protein 2 and 4 have essential overlapping functions in microfibril development
    Authors: Y Fujikawa, H Yoshida, T Inoue, T Ohbayashi, K Noda, H von Melchn, T Iwasaka, I Shiojima, TO Akama, T Nakamura
    Sci Rep, 2017;7(0):43714.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Latent TGF-beta binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5.
    Authors: Noda K, Dabovic B, Takagi K, Inoue T, Horiguchi M, Hirai M, Fujikawa Y, Akama T, Kusumoto K, Zilberberg L, Sakai L, Koli K, Naitoh M, von Melchner H, Suzuki S, Rifkin D, Nakamura T
    Proc Natl Acad Sci U S A, 2013;110(8):2852-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation.
    Authors: Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L
    J. Exp. Med., 2009;206(4):819-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. Modeling autosomal recessive cutis laxa type 1C in mice reveals distinct functions for Ltbp-4 isoforms.
    Authors: Bultmann-Mellin I, Conradi A, Maul A, Dinger K, Wempe F, Wohl A, Imhof T, Wunderlich F, Bunck A, Nakamura T, Koli K, Bloch W, Ghanem A, Heinz A, von Melchner H, Sengle G, Sterner-Kock A
    Dis Model Mech, 0;8(4):403-15.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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