Mouse Leptin/OB Antibody

Catalog #: AF498
16 Citations Datasheet / COA / SDS

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AF498
AF498-SP

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Citations (16)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Leptin/OB Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Leptin/OB in ELISAs and Western blots.

Source

Polyclonal Goat IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Leptin/OB (Catalog # 498-OB)

Immunohistochemistry

5-15 µg/mL

See below

Mouse Leptin/OB Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse Leptin/OB Biotinylated Antibody (Catalog # BAF498)

Standard: Recombinant Mouse Leptin Protein, CF (Catalog # 498-OB)

Neutralization

Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND₅₀) is typically 0.05-0.3 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse Leptin/OB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Leptin/OB in Mouse Brain. Leptin/OB was detected in perfusion fixed frozen sections of mouse brain using Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

References

Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
Tartaglia, L.A. et al. (1995) Cell 83:1263.
Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

Entrez Gene IDs

3952 (Human); 16846 (Mouse); 25608 (Rat)

Alternate Names

FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Leptin/OB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Deletion of endothelial leptin receptors in mice promotes diet-induced obesity
Authors: Gogiraju, R;Witzler, C;Shahneh, F;Hubert, A;Renner, L;Bochenek, ML;Zifkos, K;Becker, C;Thati, M;Sch�fer, K;
Scientific reports
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Obesity-associated leptin promotes chemoresistance in colorectal cancer through YAP-dependent AXL upregulation
Authors: YC Chen, CY Chien, CC Hsu, CH Lee, YT Chou, SG Shiah, SY Liu, CY Yen, AC Hsieh, M Wabitsch, YS Shieh
American journal of cancer research, 2021-09-15;11(9):4220-4240.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Ectopic Leptin Production by Intraocular Pancreatic Islet Organoids Ameliorates the Metabolic Phenotype of ob/ob Mice
Authors: B Leibiger, T Moede, I Valladolid, M Paschen, M Visa, IB Leibiger, PO Berggren
Metabolites, 2021-06-14;11(6):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Localized Antileptin Therapy Prevents Aortic Root Dilatation and Preserves Left Ventricular Systolic Function in a Murine Model of Marfan Syndrome
Authors: S Fisch, N Bachner-Hi, O Ertracht, L Guo, Y Arad, D Ben-Zvi, R Liao, J Schneiderm
J Am Heart Assoc, 2020-05-07;9(10):e014761.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells
Authors: V Frodermann, D Rohde, G Courties, N Severe, MJ Schloss, H Amatullah, CS McAlpine, S Cremer, FF Hoyer, F Ji, ID van Koever, F Herisson, L Honold, GS Masson, S Zhang, J Grune, Y Iwamoto, SP Schmidt, GR Wojtkiewic, IH Lee, K Gustafsson, G Pasterkamp, SCA de Jager, RI Sadreyev, J MacFadyen, P Libby, P Ridker, DT Scadden, K Naxerova, KL Jeffrey, FK Swirski, M Nahrendorf
Nat. Med., 2019-11-07;25(11):1761-1771.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
Oncogene, 2019-02-14;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Elevated leptin disrupts epithelial polarity and promotes premalignant alterations in the mammary gland
Authors: I Tenvooren, MZ Jenks, H Rashid, KL Cook, JK Muhlemann, C Sistrunk, J Holmes, K Wang, K Bonin, K Hodges, HW Lo, A Shaikh, IG Camarillo, SA Lelièvre, V Seewaldt, PA Vidi
Oncogene, 2019-01-22;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain
Authors: C Nozaki, E Nent, A Bilkei-Gor, A Zimmer
Sci Rep, 2018-07-17;8(1):10827.
Species: Mouse
Sample Types: In Vivo, Whole Tissue
Applications: IHC, Neutralization
Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
Authors: Q Zeng, X Luo, M Han, W Liu, H Li
EBioMedicine, 2018-06-07;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion
Authors: KT Chang, YL Lin, CT Lin, MJ Tsai, WC Huang, YH Shih, YY Lee, H Cheng, MC Huang
Data Brief, 2017-10-07;15(0):567-572.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Leptin sustains spontaneous remyelination in the adult central nervous system
Authors: K Matoba, R Muramatsu, T Yamashita
Sci Rep, 2017-01-16;7(0):40397.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.
Authors: Kim, Min Soo, Yan, Jingqi, Wu, Wenhe, Zhang, Guo, Zhang, Yalin, Cai, Dongshen
Nat Immunol, 2015-04-06;16(5):525-33.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Hypothalamic tanycytes are an ERK-gated conduit for leptin into the brain.
Authors: Balland E, Dam J, Langlet F, Caron E, Steculorum S, Messina A, Rasika S, Falluel-Morel A, Anouar Y, Dehouck B, Trinquet E, Jockers R, Bouret S, Prevot V
Cell Metab, 2014-02-04;19(2):293-301.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Leptin-dependent and leptin-independent paracrine effects of perivascular adipose tissue on neointima formation.
Authors: Schroeter M, Eschholz N, Herzberg S, Jerchel I, Leifheit-Nestler M, Czepluch F, Chalikias G, Konstantinides S, Schafer K
Arterioscler Thromb Vasc Biol, 2013-03-21;33(5):980-7.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Leptin derived from adipocytes in injured peripheral nerves facilitates development of neuropathic pain via macrophage stimulation.
Authors: Maeda T, Kiguchi N, Kobayashi Y, Ikuta T, Ozaki M, Kishioka S
Proc. Natl. Acad. Sci. U.S.A., 2009-07-20;106(31):13076-81.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
Eur. Cytokine Netw., 2002-04-01;13(2):225-9.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development