Mouse Leptin/OB Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Leptin/OB in ELISAs and Western blots. In sandwich ELISAs, approximately 40% cross-reactivity with recombinant rat Leptin/OB is observed and less than 0.2% cross-reactivity with recombinant human Leptin/OB is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Leptin/OB
    Val22-Cys167
    Accession # Q544U0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Leptin/OB (Catalog # 498-OB)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse Leptin/OB Sandwich Immunoassay
      Reagent
  • ELISA Capture
    0.2-0.8 µg/mL 
    Mouse Leptin/OB Antibody (Catalog # AF498)
  • ELISA Detection
    0.1-0.4 µg/mL 
    Mouse Leptin/OB Biotinylated Antibody (Catalog # BAF498)
  • ELISA Standard
     
    Recombinant Mouse Leptin Protein, CF (Catalog # 498-OB)
  • Neutralization
    Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND50) is typically 0.05-0.3 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse Leptin/OB.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Leptin/OB in Mouse Brain. Leptin/OB was detected in perfusion fixed frozen sections of mouse brain using Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

  • References:
    1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
    2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
    3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  • Entrez Gene IDs:
    3952 (Human); 16846 (Mouse); 25608 (Rat)
  • Alternate Names:
    FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Applications
Sample Type
  1. Leptin derived from adipocytes in injured peripheral nerves facilitates development of neuropathic pain via macrophage stimulation.
    Authors: Maeda T, Kiguchi N, Kobayashi Y, Ikuta T, Ozaki M, Kishioka S
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(31):13076-81.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo,
  2. Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
    Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
    Eur. Cytokine Netw., 2002;13(2):225-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development,
  3. Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.
    Authors: Kim, Min Soo, Yan, Jingqi, Wu, Wenhe, Zhang, Guo, Zhang, Yalin, Cai, Dongshen
    Nat Immunol, 2015;16(5):525-33.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neutralization,
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 11
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Anti-Goat HRP-AEC Cell & Tissue Staining Kit

CTS009 7
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 6
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Antigen Retrieval Reagent-Basic

CTS013 2
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Mounting Medium

CTS011 1
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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DAB Enhancer

CTS010 1
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NorthernLights Guard Mounting Media

NL996
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 127
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF109 12
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF017 11
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 3
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 2
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 2
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 3
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 4
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Donkey Anti-Goat IgG Antibody

WB AF109 4
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2
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Goat IgG VisUCyte HRP Polymer Antibody

IHC VC004
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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