Mouse Leptin R Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF497
Mouse Leptin R Biotinylated Antibody in Data
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Product Details
Citations (13)
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Mouse Leptin R Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Leptin R in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
Ala20-Gly839
Accession # Q3US58
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR)
Flow Cytometry
0.25 µg/106 cells
HEK293 human cell line transfected with mouse Leptin R and EGFP

Mouse Leptin R Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Leptin R Antibody (Catalog # MAB497)

Standard: Recombinant Mouse Leptin R Fc Chimera Protein (Catalog # 497-LR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of Leptin R in HEK293 Human Cell Line transfected with Mouse Leptin R and EGFP by Flow Cytometry. HEK293 human cell line transfected with Mouse Leptin R and EGFP was stained with (A) Goat Anti-Mouse Leptin R Biotinylated Polyclonal Antibody (Catalog # BAF497) or (B) control antibody (BAF108), followed by Allophycocyanin-conjugated Streptavidin (F0050). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

References
  1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
  4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
  5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.
Long Name
Leptin Receptor
Entrez Gene IDs
3953 (Human); 16847 (Mouse)
Alternate Names
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295

Product Datasheets

Citations for Mouse Leptin R Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Leptin receptor-expressing cells represent a distinct subpopulation of notochord-derived cells and are essential for disc homoeostasis
    Authors: B Gao, J Yin, X Xu, J Fan, D Wang, C Zheng, W Lu, P Cheng, J Sun, D Wang, L Li, BO Zhou, L Yang, Z Luo
    J Orthop Translat, 2020;21(0):91-99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors
    Authors: DD He, XT Tang, W Dong, G Cui, G Peng, X Yin, Y Chen, N Jing, BO Zhou
    Stem Cell Reports, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells
    Authors: M Zhao, F Tao, A Venkatrama, Z Li, SE Smith, J Unruh, S Chen, C Ward, P Qian, JM Perry, H Marshall, J Wang, XC He, L Li
    Cell Rep, 2019;26(3):652-669.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche
    Authors: M Balzano, M De Grandis, TP Vu Manh, L Chasson, F Bardin, A Farina, A Sergé, G Bidaut, P Charbord, L Hérault, AL Bailly, A Cartier-Mi, A Boned, M Dalod, E Duprez, P Genever, M Coles, M Bajenoff, L Xerri, M Aurrand-Li, C Schiff, SJC Mancini
    Cell Rep, 2019;26(12):3257-3271.e8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Nicotinamide mononucleotide promotes osteogenesis and reduces adipogenesis by regulating mesenchymal stromal cells via the SIRT1 pathway in aged bone marrow
    Authors: J Song, J Li, F Yang, G Ning, L Zhen, L Wu, Y Zheng, Q Zhang, D Lin, C Xie, L Peng
    Cell Death Dis, 2019;10(5):336.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Macrophage-lineage TRAP+ cells recruit periosteum-derived cells for periosteal osteogenesis and regeneration
    Authors: B Gao, R Deng, Y Chai, H Chen, B Hu, X Wang, S Zhu, Y Cao, S Ni, M Wan, L Yang, Z Luo, X Cao
    J. Clin. Invest., 2019;129(6):2578-2594.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Flow Cytometry
  7. Circulating healing (CH) cells expressing BST2 are functionally activated by the injury-regulated systemic factor HGFA
    Authors: C Lo Sicco, D Reverberi, F Villa, U Pfeffer, R Quarto, R Cancedda, R Tasso
    Stem Cell Res Ther, 2018;9(1):300.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice
    Authors: B Gao, X Lin, H Jing, J Fan, C Ji, Q Jie, C Zheng, D Wang, X Xu, Y Hu, W Lu, Z Luo, L Yang
    Aging Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Identification of Senescent Cells in the Bone Microenvironment
    Authors: Joshua N Farr
    J Bone Miner Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection
  11. High efficiency cell-specific targeting of cytokine activity.
    Authors: Garcin G, Paul F, Staufenbiel M, Bordat Y, Van der Heyden J, Wilmes S, Cartron G, Apparailly F, De Koker S, Piehler J, Tavernier J, Uze G
    Nat Commun, 2014;5(0):3016.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Leptin protects host cells from Entamoeba histolytica cytotoxicity by a STAT3-dependent mechanism.
    Authors: Marie CS, Verkerke HP, Paul SN
    Infect. Immun., 2012;80(5):1934-43.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution.
    Authors: Gruver AL, Ventevogel MS, Sempowski GD
    J. Endocrinol., 2009;203(1):75-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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