Mouse LIF Antibody

R&D Systems | Catalog # AB-449-NA

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization, In vivo assay, Reference Standard

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LIF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse LIF

Specificity

Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human LIF is observed. Neutralizes the biological activity of recombinant mouse LIF and will also neutralize the biological activity of recombinant human LIF at a 10-fold higher IgG concentration.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse LIF Antibody

IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody.

IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody.

Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA). The ND50 is typically 80-480 ng/mL.

LIF antibody in Mouse Trigeminal Ganglion by Immunohistochemistry (IHC-Fr).

LIF in Mouse Trigeminal Ganglion.

LIF was detected in perfusion fixed frozen sections of mouse trigeminal ganglion using Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to sensory neurons in trigeminal ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of LIF by Immunohistochemistry

Detection of LIF by Immunohistochemistry

Micro-CT analysis of Tail-suspension mice (A) Quantitative analysis of BV/TV, Tb.N, Tb.Th, BS/BV and Tb.Sp. (B) Micro-CT 3D reconstruction pictures. (C) Osteoclasts in the sub-growth-plate bone of tibia were stained using tartrate-resistant acid phosphatase. (D) The expression of LIF in tibias was detected by immunohistochemical staining. (E) Osteoclast numbers in Figure 1C were counted. (F) LIF positive cells in Figure 1D were counted. (F) Expression level of LIF in the serum was detected by ELISA. Black arrow: LIF positive osteocyte. All data are shown as means ± standard deviations (n = 10), *p < 0.05, **p < 0.01, ***p < 0.005 and ****p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33324677), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse LIF Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat spinal cord (dorsal roots) and mouse trigeminal ganglion

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse LIF

Neutralization

Measured by its ability to neutralize LIF-induced IL-6 secretion in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 80-480 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.

Reviewed Applications

Read 1 review rated 5 using AB-449-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIF

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Gene Symbol

LIF

Additional LIF Products

Product Documents for Mouse LIF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse LIF Antibody

For research use only

Citations for Mouse LIF Antibody

Customer Reviews for Mouse LIF Antibody (1)

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  • Mouse LIF Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Mouse iPS
    Species: Mouse
    Verified Customer | Posted 05/17/2017
    Mouse LIF Antibody AB-449-NA

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Protocols

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