Mouse LIF Antibody

Catalog # Availability Size / Price Qty
AB-449-NA
IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)

Mouse LIF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human LIF is observed. Neutralizes the biological activity of recombinant mouse LIF and will also neutralize the biological activity of recombinant human LIF at a 10-fold higher IgG concentration.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant mouse LIF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse LIF
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize LIF-induced IL-6 secretion in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 80-480 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. View Larger

IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA). The ND50 is typically 80-480 ng/mL.

Immunohistochemistry LIF antibody in Mouse Trigeminal Ganglion by Immunohistochemistry (IHC-Fr). View Larger

LIF in Mouse Trigeminal Ganglion. LIF was detected in perfusion fixed frozen sections of mouse trigeminal ganglion using Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to sensory neurons in trigeminal ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIF

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Long Name
Leukemia Inhibitory Factor
Entrez Gene IDs
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

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Citations for Mouse LIF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production
    Authors: Kakae, M;Nakajima, H;Tobori, S;Kawashita, A;Miyanohara, J;Morishima, M;Nagayasu, K;Nakagawa, T;Shigetomi, E;Koizumi, S;Mori, Y;Kaneko, S;Shirakawa, H;
    Science advances
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: In Vivo, Neutralization
  2. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis
    Authors: S Xu, X Yang, Q Chen, Z Liu, Y Chen, X Yao, A Xiao, J Tian, L Xie, M Zhou, Z Hu, F Zhu, X Xu, F Hou, J Nie
    EBioMedicine, 2022-11-04;86(0):104312.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, In Vivo
  3. KAI1(CD82) is a key molecule to control angiogenesis and switch angiogenic milieu to quiescent state
    Authors: JW Lee, J Hur, YW Kwon, CW Chae, JI Choi, I Hwang, JY Yun, JA Kang, YE Choi, YH Kim, SE Lee, C Lee, DH Jo, H Seok, BS Cho, SH Baek, HS Kim
    Journal of hematology & oncology, 2021-09-16;14(1):148.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Sclerostin expression in trabecular bone is downregulated by osteoclasts
    Authors: M Koide, T Yamashita, K Murakami, S Uehara, K Nakamura, M Nakamura, M Matsushita, T Ara, H Yasuda, JM Penninger, N Takahashi, N Udagawa, Y Kobayashi
    Sci Rep, 2020-08-13;10(1):13751.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Mapping the Effects of Genetic Variation on Chromatin State and Gene Expression Reveals Loci That Control Ground State Pluripotency
    Authors: DA Skelly, A Czechanski, C Byers, S Aydin, C Spruce, C Olivier, K Choi, DM Gatti, N Raghupathy, GR Keele, A Stanton, M Vincent, S Dion, I Greenstein, M Pankratz, DK Porter, W Martin, C O'Connor, W Qin, AH Harrill, T Choi, GA Churchill, SC Munger, CL Baker, LG Reinholdt
    Cell Stem Cell, 2020-08-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization, Reference Standard
  6. Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy
    Authors: SS Welc, I Flores, M Wehling-He, J Ramos, Y Wang, C Bertoni, JG Tidball
    Nat Commun, 2019-06-26;10(1):2788.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin
    Authors: GK Arora, A Gupta, S Narayanan, T Guo, P Iyengar, RE Infante
    JCI Insight, 2018-07-26;3(14):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate
    Authors: Emma L Rawlins
    Development, 2016-08-30;143(20):3686-3699.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-signaling cytokines in rodents.
    Authors: Kanazawa H, Ieda M, Kimura K, Arai T, Kawaguchi-Manabe H, Matsuhashi T, Endo J, Sano M, Kawakami T, Kimura T, Monkawa T, Hayashi M, Iwanami A, Okano H, Okada Y, Ishibashi-Ueda H, Ogawa S, Fukuda K
    J. Clin. Invest., 2010-01-04;120(2):408-21.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Mouse LIF Antibody
By Anonymous on 05/17/2017
Application: B/N Sample Tested: Mouse iPS Species: Mouse