Mouse LIF Antibody

Catalog # Availability Size / Price Qty
AF449
AF449-SP

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IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody.
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Citations (11)
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Mouse LIF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than
5% cross‑reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross‑reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.  
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse LIF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL

Recombinant Mouse LIF

Neutralization
Measured by its ability to neutralize LIF-induced IL-6 secretion in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 10-60 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. View Larger

IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND50 is typically 10-60 ng/mL.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIF

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Long Name
Leukemia Inhibitory Factor
Entrez Gene IDs
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

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Citations for Mouse LIF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice
    Authors: Wei Cai, Ligen Shi, Jingyan Zhao, Fei Xu, Connor Dufort, Qing Ye et al.
    Journal of Clinical Investigation
  2. Concomitant Activation of OSM and LIF Receptor by a Dual-Specific hlOSM Variant Confers Cardioprotection after Myocardial Infarction in Mice
    Authors: H Lörchner, JM Adrian-Seg, C Waechter, R Wagner, ME Góes, N Brachmann, K Sreenivasa, A Wietelmann, S Günther, N Doll, T Braun, J Pöling
    International Journal of Molecular Sciences, 2021-12-29;23(1):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. IL-1-induced JAK/STAT signaling is antagonized by TGF-beta to shape CAF heterogeneity in pancreatic ductal adenocarcinoma.
    Authors: Giulia Biffi, Tobiloba E. Oni, Benjamin Spielman, Yuan Hao, Ela Elyada, Youngkyu Park et al.
    Cancer Discovery
  4. Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210
    Authors: Y Tang, S Xiong, P Yu, F Liu, L Cheng
    Cell Rep, 2018-07-31;24(5):1355-1362.e3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCs
    Authors: H Won, D Moreira, C Gao, P Duttagupta, X Zhao, E Manuel, D Diamond, YC Yuan, Z Liu, J Jones, M D'Apuzzo, S Pal, M Kortylewsk
    J. Leukoc. Biol., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Protein Kinase Inhibitor gamma Reciprocally Regulates Osteoblast and Adipocyte Differentiation by Downregulating Leukemia Inhibitory Factor
    Authors: Xin Chen, Bryan S. Hausman, Guangbin Luo, Guang Zhou, Shunichi Murakami, Janet Rubin et al.
    Stem Cells
  7. Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity.
    Authors: Moidunny S, Vinet J
    J Neuroinflammation, 2012-08-16;9(1):198.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  8. Leukemia inhibitory factor signaling is required for lung protection during pneumonia.
    Authors: Quinton LJ, Mizgerd JP, Hilliard KL
    J. Immunol., 2012-05-11;188(12):6300-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune disease.
    Authors: Cao W, Yang Y, Wang Z, Liu A, Fang L, Wu F, Hong J, Shi Y, Leung S, Dong C, Zhang JZ
    Immunity, 2011-08-11;35(2):273-84.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Astrocytes promote myelination in response to electrical impulses.
    Authors: Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD
    Neuron, 2006-03-16;49(6):823-32.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  11. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001-11-01;293(10):508-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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