hh Mouse LIF Antibody AF449: R&D Systems

Mouse LIF Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than
    5% cross‑reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross‑reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.  
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse LIF
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL

    Recombinant Mouse LIF

  • Neutralization
    Measured by its ability to neutralize LIF-induced IL-6 secretion in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 10-60 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND50 is typically 10-60 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIF

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

  • Long Name:
    Leukemia Inhibitory Factor
  • Entrez Gene IDs:
    3976 (Human); 16878 (Mouse); 403449 (Canine)
  • Alternate Names:
    CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCs
    Authors: H Won, D Moreira, C Gao, P Duttagupta, X Zhao, E Manuel, D Diamond, YC Yuan, Z Liu, J Jones, M D'Apuzzo, S Pal, M Kortylewsk
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Leukemia inhibitory factor signaling is required for lung protection during pneumonia.
    Authors: Quinton LJ, Mizgerd JP, Hilliard KL
    J. Immunol., 2012;188(12):6300-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity.
    Authors: Moidunny S, Vinet J
    J Neuroinflammation, 2012;9(1):198.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  4. Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune disease.
    Authors: Cao W, Yang Y, Wang Z, Liu A, Fang L, Wu F, Hong J, Shi Y, Leung S, Dong C, Zhang JZ
    Immunity, 2011;35(2):273-84.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. Astrocytes promote myelination in response to electrical impulses.
    Authors: Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD
    Neuron, 2006;49(6):823-32.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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