Mouse LIF Antibody

IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody.

Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND₅₀ is typically 10-60 ng/mL.

Detection of LIF by Western Blot

Kinetic expression pattern of OSM, LIF and their corresponding receptors OSMR and LIFR in cardiac tissue after the onset of myocardial infarction in mice. (A) Schematic illustration of kinetic expression profiling. Cardiac tissue was harvested at indicated time points post-MI and fractionated into a non-infarcted remote zone (RZ) and infarction zone (IZ). Expression of OSMR, OSM, LIFR and LIF in RZ and IZ was analyzed by immunoblotting (n = 6 at Day 1; n = 5 at Day 2; n = 7 at Day 4; n = 5 at Day 7; n = 5 at Day 14 post-MI). (B) Two representative immunoblots of OSMR, OSM, LIFR and LIF in RZ and IZ at indicated time points post-MI. (C–F) Semi-quantitative analysis of immunoblots shown in (B) based on the adjusted mean volume pixel density of bands. Statistical analysis was performed by two-way ANOVA. Asterisks indicate Bonferroni post-hoc test significances between RZ and IZ at individual time points as well as between RZ and IZ throughout the period of observation with * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Data are presented as mean ± sem. Immunofluorescence analysis of (G) OSMR and (H) LIFR in infarcted hearts of mice 7 days after MI. F-actin: grey. OSMR: red. LIFR: blue. IZ: infarction zone. RZ: remote zone. RV: right ventricle. LV: left ventricle. Scale bars, 500 µm and 100 µm in magnified sections. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35008777), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of LIF by Western Blot

Collagenase-1 treatment increases NK cell infiltration and expression of the cytokine LIF in spontaneous pregnancy model implantation sites.(A) A representative flow cytometry image showing the gating strategy used to identify NK cells (CD45+CD3 epsilon −NK1.1+NKp46+). (B) Quantification of flow cytometry results showing the percent of NK cells of live cells at E4.5 (n ≥ 12). (C) Representative Western blot (left) and quantification analysis (right) showing expression of LIF and NKp46 in E4.5 implantation sites. Western blot quantification performed using ImageJ analysis tool (n = 3). NKs, natural killer cells. (D) Real-time PCR analysis of cytokines’ transcripts at E4.5 (n = 8). Data were analyzed by an unpaired, two-tailed t test. Results are presented as mean ± SEM with significance: *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39151945), licensed under a CC-BY license. Not internally tested by R&D Systems.