Mouse LIF Quantikine ELISA Kit

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MLF00
Mouse LIF ELISA Standard Curve
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Product Details
Procedure
Citations (12)
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Reviews (4)

Mouse LIF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
Sensitivity
3.13 pg/mL
Assay Range
21.9 - 1,400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse LIF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse LIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIF levels in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse LIF and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse LIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse LIF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 80 278 651 87 278 656
Standard Deviation 4.1 24 50 5 12 35
CV% 5.1 8.6 7.7 5.7 4.3 5.3

Recovery

The recovery of mouse LIF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 98 81-108
EDTA Plasma (n=6) 97 87-110
Serum (n=5) 96 90-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse LIF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse LIF ELISA Linearity

Data Examples

Mouse LIF ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LIF

Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.

Long Name:
Leukemia Inhibitory Factor
Entrez Gene IDs:
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names:
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse LIF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy
    Authors: P Li, Q Li, N Biswas, H Xin, T Diemer, L Liu, L Perez Guti, G Paternostr, C Piermarocc, S Domanskyi, RK Wang, N Ferrara
    Embo Molecular Medicine, 2021;0(0):e14511.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  2. KAI1(CD82) is a key molecule to control angiogenesis and switch angiogenic milieu to quiescent state
    Authors: JW Lee, J Hur, YW Kwon, CW Chae, JI Choi, I Hwang, JY Yun, JA Kang, YE Choi, YH Kim, SE Lee, C Lee, DH Jo, H Seok, BS Cho, SH Baek, HS Kim
    Journal of hematology & oncology, 2021;14(1):148.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Astrocyte-Derived Estrogen Regulates Reactive Astrogliosis and is Neuroprotective Following Ischemic Brain Injury
    Authors: J Wang, GR Sareddy, Y Lu, UP Pratap, F Tang, KM Greene, PL Meyre, RR Tekmal, RK Vadlamudi, DW Brann
    J Neurosci, 2020;0(0):.
    Species: Mouse
    Sample Types: Tissue Lysates
  4. Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy
    Authors: SS Welc, I Flores, M Wehling-He, J Ramos, Y Wang, C Bertoni, JG Tidball
    Nat Commun, 2019;10(1):2788.
    Species: Mouse
    Sample Types: Serum
  5. Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells
    Authors: M Shoji, H Minato, S Ogaki, M Seki, Y Suzuki, S Kume, T Kuzuhara
    PLoS ONE, 2018;13(7):e0201239.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction
    PLoS ONE, 2016;11(5):e0156562.
    Species: Mouse
    Sample Types: Plasma
  7. Improving embryonic stem cell expansion through the combination of perfusion and Bioprocess model design.
    Authors: Yeo D, Kiparissides A, Cha J, Aguilar-Gallardo C, Polak J, Tsiridis E, Pistikopoulos E, Mantalaris A
    PLoS ONE, 2013;8(12):e81728.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Leukemia inhibitory factor signaling is required for lung protection during pneumonia.
    Authors: Quinton LJ, Mizgerd JP, Hilliard KL
    J. Immunol., 2012;188(12):6300-8.
    Species: Mouse
    Sample Types: BALF
  9. Blocking LIF action in the uterus by using a PEGylated antagonist prevents implantation: a nonhormonal contraceptive strategy.
    Authors: White CA, Zhang JG, Salamonsen LA, Baca M, Fairlie WD, Metcalf D, Nicola NA, Robb L, Dimitriadis E
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(49):19357-62.
    Species: Mouse
    Sample Types: Serum
  10. Astrocytes promote myelination in response to electrical impulses.
    Authors: Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD
    Neuron, 2006;49(6):823-32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Leukaemia inhibitory factor (LIF) is functionally linked to axotrophin and both LIF and axotrophin are linked to regulatory immune tolerance.
    Authors: Metcalfe SM, Muthukumarana PA, Thompson HL, Haendel MA, Lyons GE
    FEBS Lett., 2005;579(3):609-14.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse LIF Quantikine ELISA Kit

Average Rating: 4.8 (Based on 4 Reviews)

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Mouse LIF Quantikine ELISA Kit
By Anonymous on 07/23/2021
Sample Tested: moues serum

Mouse LIF Quantikine ELISA Kit
By Anonymous on 04/08/2019
Sample Tested: Serum

Mouse LIF Quantikine ELISA Kit
By Anonymous on 10/06/2016
Sample Tested: Cell culture supernatant

Mouse LIF Quantikine ELISA Kit
By Anonymous on 09/30/2016
Sample Tested: Cell Culture Media