Mouse LIF Quantikine ELISA Kit

(6 citations)
(2 Reviews)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
  • Sensitivity
    3.13 pg/mL
  • Assay Range
    21.9 - 1,400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse LIF
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse LIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIF levels in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse LIF and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse LIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse LIF.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4.1245051235


The recovery of mouse LIF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 98 81-108
EDTA Plasma (n=6) 97 87-110
Serum (n=5) 96 90-108
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse LIF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse LIF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LIF
Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.
    • Long Name
      Leukemia Inhibitory Factor
    • Entrez Gene IDs
      3976 (Human); 16878 (Mouse); 403449 (Canine);
    • Alternate Names
      CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; Melanoma-derived LPL inhibitor; MLPLI;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 6 of 6
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    Sample Type
    1. Improving embryonic stem cell expansion through the combination of perfusion and Bioprocess model design.
      Authors: Yeo D, Kiparissides A, Cha J, Aguilar-Gallardo C, Polak J, Tsiridis E, Pistikopoulos E, Mantalaris A
      PLoS ONE, 2013;8(12):e81728.
      Species: Mouse
      Sample Type: cell culture supernate
    2. Leukemia inhibitory factor signaling is required for lung protection during pneumonia.
      Authors: Quinton LJ, Mizgerd JP, Hilliard KL
      J. Immunol., 2012;188(12):6300-8.
      Species: Mouse
      Sample Type: BALF
    3. Blocking LIF action in the uterus by using a PEGylated antagonist prevents implantation: a nonhormonal contraceptive strategy.
      Authors: White CA, Zhang JG, Salamonsen LA, Baca M, Fairlie WD, Metcalf D, Nicola NA, Robb L, Dimitriadis E
      Proc. Natl. Acad. Sci. U.S.A., 2007;104(49):19357-62.
      Species: Mouse
      Sample Type: Serum
    4. Astrocytes promote myelination in response to electrical impulses.
      Authors: Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD
      Neuron, 2006;49(6):823-32.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    5. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
      Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
      Transplantation, 2005;79(6):726-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. Leukaemia inhibitory factor (LIF) is functionally linked to axotrophin and both LIF and axotrophin are linked to regulatory immune tolerance.
      Authors: Metcalfe SM, Muthukumarana PA, Thompson HL, Haendel MA, Lyons GE
      FEBS Lett., 2005;579(3):609-14.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
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    Average Rating: 5 (Based on 2 reviews)

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    Images Ratings Applications Species Reviewed By Date Details
     Mouse LIF Quantikine ELISA Kit MLF00
      Anonymous 10/06/2016
    Image Details
     Mouse LIF Quantikine ELISA Kit MLF00
    : Mouse LIF Quantikine ELISA Kit [MLF00]


    Sample TestedCell culture supernatant
      Anonymous 09/30/2016


    Sample TestedCell Culture Media

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