Limitin, also known as IFN-zeta, is a 20 kDa glycoprotein that is secreted by T cells and bronchial and salivary duct epithelial cells. It inhibits B-lineage cell development but has little effect on myelopoiesis or erythropoiesis. Limitin binds to IFN-alpha / beta receptors and has potent antiviral activity. Mouse Limitin shares 32%, 26%, and 30% amino acid sequence identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.
Mouse Limitin/IFN-zeta Antibody (183707)
R&D Systems | Catalog # MAB5971
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
ELISA Capture (Matched Antibody Pair), Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 183707
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Limitin/IFN‑ zeta
Leu22-Arg182
Accession # Q9R1T0
Leu22-Arg182
Accession # Q9R1T0
Specificity
Detects mouse Limitin/IFN‑ zeta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse Limitin/IFN-zeta Antibody (183707)
Mouse Limitin / IFN-zeta ELISA Standard Curve
Recombinant Mouse Limitin/IFN‑ zeta (Catalog # 1535-LM) was serially diluted and captured by Rat Anti-Mouse Limitin/IFN‑ zeta Monoclonal Antibody (Catalog # MAB5971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Limitin/IFN‑ zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF597) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse Limitin/IFN-zeta Antibody (183707)
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Limitin inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin/IFN‑ zeta (Catalog # 1535-LM).
Mouse Limitin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Limitin/IFN-zeta
Long Name
Interferon zeta
Alternate Names
IFN-z, IFNzeta
Entrez Gene IDs
319146 (Mouse)
Gene Symbol
IFNZ
UniProt
Additional Limitin/IFN-zeta Products
Product Documents for Mouse Limitin/IFN-zeta Antibody (183707)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Limitin/IFN-zeta Antibody (183707)
For research use only
Related Research Areas
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Associated Pathways
