Mouse Limitin/IFN-zeta Antibody

Catalog # Availability Size / Price Qty
MAB5971
MAB5971-SP
Product Details
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Mouse Limitin/IFN-zeta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Limitin/IFN‑ zeta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma is observed.
Source
Monoclonal Rat IgG2A Clone # 183707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Limitin/IFN‑ zeta
Leu22-Arg182
Accession # Q9R1T0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample

Mouse Limitin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Limitin/IFN-zeta Biotinylated Antibody (Catalog # BAF597)

Standard: Recombinant Mouse Limitin/IFN-zeta Protein (Catalog # 1535-LM)

Neutralization
Measured by its ability to neutralize Limitin inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Limitin/IFN-zeta

Limitin, also known as IFN-zeta, is a 20 kDa glycoprotein that is secreted by T cells and bronchial and salivary duct epithelial cells. It inhibits B-lineage cell development but has little effect on myelopoiesis or erythropoiesis. Limitin binds to IFN-alpha / beta receptors and has potent antiviral activity. Mouse Limitin shares 32%, 26%, and 30% amino acid sequence identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.

Long Name
Interferon zeta
Entrez Gene IDs
319146 (Mouse)
Alternate Names
6030405N23Rik; BGIF; Ifnz; IFN-z; IFNzeta; interferon zeta; Limitin; Lmtn

Product Datasheets

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