Mouse Limitin/IFN-zeta Biotinylated Antibody

Catalog #: BAF597 Datasheet / COA / SDS

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Mouse Limitin/IFN-zeta Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects Limitin/IFN‑ zeta in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) IFN-gamma and rmIFN-alpha / beta R1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Limitin/IFN‑ zeta
Leu22-Arg182
Accession # Q9R1T0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Limitin (Catalog # 1535-LM)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse thymus

Mouse Limitin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Limitin/IFN-zeta Antibody (Catalog # MAB5971)

Standard: Recombinant Mouse Limitin/IFN-zeta Protein (Catalog # 1535-LM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Limitin/IFN-zeta

Limitin, also known as IFN-zeta, is a 20 kDa glycoprotein that is secreted by T cells and bronchial and salivary duct epithelial cells. It inhibits B-lineage cell development but has little effect on myelopoiesis or erythropoiesis. Limitin binds to IFN-alpha / beta receptors and has potent antiviral activity. Mouse Limitin shares 32%, 26%, and 30% amino acid sequence identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.

Long Name

Interferon zeta

Entrez Gene IDs

319146 (Mouse)

Alternate Names

6030405N23Rik; BGIF; Ifnz; IFN-z; IFNzeta; interferon zeta; Limitin; Lmtn