Mouse M-CSF Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human M-CSF or recombinant mouse SCF is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 131614
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse M-CSF
    Lys33-Glu262 (predicted)
    Accession # P07141
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse M-CSF (Catalog # 416-ML)
  • Neutralization
    Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous luekemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 10 ng/mL Recombinant Mouse M‑CSF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody. Recombinant Mouse M‑CSF (Catalog # 416-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M‑CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse M‑CSF Monoclonal Antibody (Catalog # MAB4161). The ND50 is typically 3-12 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

  • References:
    1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
    2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
    3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
    4. Ryan, G.R. et al. (2001) Blood 98:74.
    5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
    6. Nandi, S. et al. (2006) Blood 107:786.
    7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
    8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
    9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
    10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
    11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
    12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
    13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.
  • Long Name:
    Macrophage Colony Stimulating Factor
  • Entrez Gene IDs:
    1435 (Human); 12977 (Mouse)
  • Alternate Names:
    colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. An inflammatory gene signature distinguishes neurofibroma Schwann cells and macrophages from cells in the normal peripheral nervous system
    Authors: K Choi, K Komurov, JS Fletcher, E Jousma, JA Cancelas, J Wu, N Ratner
    Sci Rep, 2017;7(0):43315.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.
    Authors: Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley E, Kanayama N, Mabbott N, Magari M, Ohmori H
    J Leukoc Biol, 2014;95(1):19-31.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival.
    Authors: Luo, Jian, Elwood, Fiona, Britschgi, Markus, Villeda, Saul, Zhang, Hui, Ding, Zhaoqing, Zhu, Liyin, Alabsi, Haitham, Getachew, Ruth, Narasimhan, Ramya, Wabl, Rafael, Fainberg, Nina, James, Michelle, Wong, Gordon, Relton, Jane, Gambhir, Sanjiv S, Pollard, Jeffrey, Wyss-Coray, Tony
    J Exp Med, 2013;210(1):157-72.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  4. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007;109(9):3706-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Rat IgG2B Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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