Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry-Frozen, Western Blot, Neutralization, Activation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 131614
View all M-CSF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse M-CSF
Lys33-Glu262 (predicted)
Accession # P07141

Specificity

Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human M-CSF or recombinant mouse SCF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse M-CSF Antibody (131614)

Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody.

Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody.

Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse M-CSF Monoclonal Antibody (Catalog # MAB4161). The ND50 is typically 3-12 µg/mL.
Detection of Mouse M-CSF by Immunocytochemistry/Immunofluorescence

Detection of Mouse M-CSF by Immunocytochemistry/Immunofluorescence

Injury-induced SSEA-1 positive cells were regulated by topical application of M-CSF or M-CSF neutralizing antibody.Skin wounds in mice were treated by either PBS control (top panels) or topical application of recombinant M-CSF protein (middle panels) or topical application of M-CSF neutralizing antibody for 6 days. This figure showed a representative skin section from 6 wounds in each group, stained with SSEA-1 (red color). DAPI (blue) was used for cell nucleus staining. Scale bars, 100 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28979), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse M-CSF by Immunocytochemistry/Immunofluorescence

Detection of Mouse M-CSF by Immunocytochemistry/Immunofluorescence

SSEA-1 positive cells are also SSEA-3 positive and express M-CSF receptor.(A) Injured skin sections were stained with SSEA-1 (red color) and SSEA-3 (green color) antibodies. IgG was used for a negative staining control. DAPI (blue) was used for cell nucleus staining. Scale bars, 50 μm. (B) Injured skin sections were stained with SSEA-1 (green color) and M-CSF receptor (red color) antibodies. DAPI (blue) was used for cell nucleus staining. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28979), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse M-CSF Antibody (131614)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse M-CSF (Catalog # 416-ML)

Neutralization

Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous luekemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 10 ng/mL Recombinant Mouse M‑CSF.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

References

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

UniProt

P07141

Additional M-CSF Products

Product Documents for Mouse M-CSF Antibody (131614)

Certificate of Analysis

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Product Specific Notices for Mouse M-CSF Antibody (131614)

For research use only

Citations for Mouse M-CSF Antibody (131614)

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