Mouse MBL-1 Antibody

Catalog # Availability Size / Price Qty
AF2077
AF2077-SP

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Product Details
Citations (3)
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Mouse MBL-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MBL-1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human MBL and recombinant mouse MBL-2 is observed under reducing conditions.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MBL-1 (R&D Systems, Catalog # 2077-MB)
Ser18-Ala239
Accession # P39039
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse MBL‑1 (Catalog # 2077-MB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBL-1

Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which binds to an array of carbohydrate patterns on pathogen surfaces (1, 2). Collectin family members share common structural features: a cysteine rich amino-terminal domain, a collagen-like region, an alpha -helical coiled-coil neck domain and a carboxy terminal C-type (Ca++-dependent) lectin or carbohydrate recognition domain (CRD). MBL homotrimerizes to form a structural unit joined by N-terminal disulfide bridges. These homotrimers further associate into oligomeric structures of up to 6 units. Whereas two forms of MBL proteins (MBL-1, also known as S-MBP or MBL-A and MBL-2, also known as L-MBP or MBL-C) exist in rodents and other animals, only one functional MBL protein is present in humans. Mouse MBL-1 shares about 52% and 60% amino acid sequence identity with mouse MBL-2 and human MBL, respectively.

In mouse, MBL-1 and MBL-2 are the only collectins that can activate complement via the lectin complement pathway (1, 2). Serum oligomeric MBL associates with MBL-associated serine protease (MASP) proenzymes. The MBL-MASP proenzyme complex preferentially interacts with sugar patterns containing mannose, glucose,
L‑fucose, or N-acetyl-glucosamine present at a terminal nonreducing postion on the cell surface of various pathogens and certain tumor cells. This interaction induces pro-enzyme activation and the triggering of the complement cascade, resulting in opsonization and pathogen removal via humoral and cellular immune responses. MBL does not recognize self-components or glycoproteins from other higher animals due to the presence of terminal sialic acid or galactose that interrupts the repeating carbohydrate structures (3). A number of membrane receptors for MBL, including C1q phagocytic receptor (C1qRp), calreticulin (also known as C1qR), and CR1 (CD35), have been described. Interactions with these receptors may also be important in stimulating phagocytosis (1, 2).

Mouse MBL-1 and MBL-2 are produced primarily in the liver and are secreted into the blood stream. In addition, mouse MBL-1 is also expressed in lung, kidney and testis while MBL-2 is expressed in kidney, thymus, and small intestine (1, 4, 5).

References
  1. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547. 
  2. Fujita, R. et al. (2004) Immunol. Rev. 198:185.
  3. Saevarsdottir, S. et al. (2004) Scand. J. Immnunol. 60:23.
  4. Uemura, K. et al. (2002) J. Immunol. 169:6945.
  5. Wagner, S. et al. (2003) J. Immunol. 170:1462.
Long Name
Mannose Binding Lectin
Entrez Gene IDs
17194 (Mouse)
Alternate Names
MBL1; MBL-1; MBL-A; MBP-A; p28b; RaRF p28B; S-MBP

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Citations for Mouse MBL-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Murine T Cell Maturation Entails Protection from MBL2, but Complement Proteins Do Not Drive Clearance of Cells That Fail Maturation in the Absence of NKAP
    Authors: B Dash, PJ Belmonte, SR Fine, MJ Shapiro, JY Chung, AD Schwab, SA McCue, MJ Rajcula, VS Shapiro
    J. Immunol., 2019-06-07;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  2. Transglutaminase is essential for IgA nephropathy development acting through IgA receptors.
    Authors: Berthelot L, Papista C, Maciel TT, Biarnes-Pelicot M, Tissandie E, Wang PH, Tamouza H, Jamin A, Bex-Coudrat J, Gestin A, Boumediene A, Arcos-Fajardo M, England P, Pillebout E, Walker F, Daugas E, Vrtosvnik F, Flamant M, Benhamou M, Cogne M, Moura IC, Monteiro RC
    J. Exp. Med., 2012-03-26;209(4):793-806.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Mannose binding lectin is required for alphavirus-induced arthritis/myositis.
    Authors: Gunn BM, Morrison TE, Whitmore AC
    PLoS Pathog., 2012-03-22;8(3):e1002586.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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