Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 345404
View all Melanocortin-3 R/MC3R Primary Antibodies »

Product Specifications

Immunogen

Y3 rat myeloid cell line transfeced with mouse Melanocortin 3R/MC3R

Specificity

Detects Melanocortin 3R/MC3R in direct ELISAs and Western blots. Stains mouse Melanocortin 3R/MC3R-transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Melanocortin-3 R/MC3R Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse Melanocortin 3R/MC3R transfected HEK293 cells

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Melanocortin-3 R/MC3R

Mouse Melanocortin 3R (MC3R) is a member of the melanocortin receptor family. The 323 amino acid G-protein-coupled 7-transmembrane glycoprotein is expressed in the brain where, along with MC4R, it binds alpha, beta and gamma melanocyte stimulating hormones and is involved in regulating energy balance. MC3R is also expressed by macrophages and plays an anti-inflammatory role in the heart and other tissues. Of the 69 aa that are extracellular, mouse MC3R shares 91% and 78% sequence identity with rat and human MC3R, respectively.

Long Name

Melanocortin 3 Receptor

Alternate Names

MC3R, Melanocortin-3R, Melanocortin3R

Entrez Gene IDs

4159 (Human); 17201 (Mouse); 29310 (Rat)

Gene Symbol

MC3R

Additional Melanocortin-3 R/MC3R Products

Product Documents for Mouse Melanocortin-3 R/MC3R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Melanocortin-3 R/MC3R Antibody

For research use only

Citations for Mouse Melanocortin-3 R/MC3R Antibody

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