Mouse Melanocortin-3 R/MC3R Antibody

Catalog # Availability Size / Price Qty
MAB3737
MAB3737-SP
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Mouse Melanocortin-3 R/MC3R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects Melanocortin 3R/MC3R in direct ELISAs and Western blots. Stains mouse Melanocortin 3R/MC3R-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 345404
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfeced with mouse Melanocortin 3R/MC3R
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse Melanocortin 3R/MC3R transfected HEK293 cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melanocortin-3 R/MC3R

Mouse Melanocortin 3R (MC3R) is a member of the melanocortin receptor family. The 323 amino acid G-protein-coupled 7-transmembrane glycoprotein is expressed in the brain where, along with MC4R, it binds alpha, beta and gamma melanocyte stimulating hormones and is involved in regulating energy balance. MC3R is also expressed by macrophages and plays an anti-inflammatory role in the heart and other tissues. Of the 69 aa that are extracellular, mouse MC3R shares 91% and 78% sequence identity with rat and human MC3R, respectively.

Long Name
Melanocortin 3 Receptor
Entrez Gene IDs
4159 (Human); 17201 (Mouse); 29310 (Rat)
Alternate Names
BMIQ9; MC3; MC3R; MC3-R; melanocortin 3 receptor; melanocortin receptor 3; Melanocortin-3 R; Melanocortin3R; Melanocortin-3R; OB20; Obesity quantitative trait locus; OQTL

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