Mouse Mer Antibody

Catalog # Availability Size / Price Qty
MAB591-SP
MAB591-500
MAB591-100
Mer in J774A.1 Mouse Cell Line.
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Product Details
Citations (3)
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Mouse Mer Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Mer in direct ELISAs and Western blots. Does not cross-react with recombinant human Mer, recombinant mouse (rm) Axl, or rmDtk.
Source
Monoclonal Rat IgG1 Clone # 108921
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Mer Fc Chimera (Catalog # 591-MR)
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Mer antibody in J774A by Immunocytochemistry (ICC).1 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Mer in J774A.1 Mouse Cell Line. Mer was detected in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line using Rat Anti-Mouse Mer Monoclonal Antibody (Catalog # MAB591) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1-2).

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

Product Datasheets

Citations for Mouse Mer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4(+)CD25(+) Regulatory T Cells Mainly through Axl Receptor
    Authors: GJ Zhao, JY Zheng, JL Bian, LW Chen, N Dong, Y Yu, GL Hong, A Chandoo, YM Yao, ZQ Lu
    Mediators Inflamm, 2017;2017(0):6848430.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
    Authors: Deng T, Zhang Y, Chen Q, Yan K, Han D
    Immunology, 2012;135(1):40-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Toll-like receptor-initiated testicular innate immune responses in mouse Leydig cells.
    Authors: Shang T, Zhang X, Wang T, Sun B, Deng T, Han D
    Endocrinology, 2011;152(7):2827-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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