MIXL1 in D3 Mouse Embryonic Stem Cells. MIXL1 was detected in immersion fixed D3 mouse embryonic stem cells differentiated by LIF withdrawal for 3 days using Rat Anti-Mouse MIXL1 Monoclonal Antibody (Catalog # MAB8099) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei in a subset of differentiating cells. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MIXL1 (Mix-like 1) is a 36 kDa member of the paired-like homeodomain protein family. It is expressed in the primitive streak of the gastrulating embryo, and marks cells destined to form mesoderm and endoderm. It is also expressed in differentiating embryonic stem cells, immature B and T lymphocytes during normal hematopoiesis, and several B, T or myeloid lineage acute leukemias. The 231 amino acid (aa) human MIXL1 contains a homeobox domain at aa 86-145. The mouse MIXL1 immunogen (aa 147-231) shares 72% and 91% aa sequence identity with human and rat MIXL1, respectively.
Mix1 Homeobox-like 1
Entrez Gene IDs:
83881 (Human); 27217 (Mouse)
hMix; homeobox protein MIXL1; Homeodomain protein MIX; MGC138179; MILD1; Mix.1 homeobox-like protein; Mix1 homeobox (Xenopus laevis)-like 1; Mix1 homeobox-like 1 (Xenopus laevis); MIX1 homeobox-like protein 1; Mix-like homeobox protein 1; MIXLMIX
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