Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 868132
View all MIXL1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse MIXL1
Lys147-Phe231
Accession # Q9WUI0

Specificity

Detects mouse MIXL1 in ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse MIXL1 Antibody

MIXL1 antibody in D3 Mouse Embryonic Stem Cells by Immunocytochemistry (ICC).

MIXL1 in D3 Mouse Embryonic Stem Cells.

MIXL1 was detected in immersion fixed D3 mouse embryonic stem cells differentiated by LIF withdrawal for 3 days using Rat Anti-Mouse MIXL1 Monoclonal Antibody (Catalog # MAB8099) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei in a subset of differentiating cells. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Mouse MIXL1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cells differentiated by LIF withdrawal for 3 days

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIXL1

MIXL1 (Mix-like 1) is a 36 kDa member of the paired-like homeodomain protein family. It is expressed in the primitive streak of the gastrulating embryo, and marks cells destined to form mesoderm and endoderm. It is also expressed in differentiating embryonic stem cells, immature B and T lymphocytes during normal hematopoiesis, and several B, T or myeloid lineage acute leukemias. The 231 amino acid (aa) human MIXL1 contains a homeobox domain at aa 86-145.  The mouse MIXL1 immunogen (aa 147-231) shares 72% and 91% aa sequence identity with human and rat MIXL1, respectively.

Long Name

Mix1 Homeobox-like 1

Alternate Names

MILD1

Entrez Gene IDs

83881 (Human); 27217 (Mouse)

Gene Symbol

MIXL1

UniProt

Q9WUI0 (Rat)

Additional MIXL1 Products

Product Documents for Mouse MIXL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse MIXL1 Antibody

For research use only

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