Mouse MyD88 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MyD88 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 316902
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse MyD88
    Met1-Pro296
    Accession # Q3U7M4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
MyD88 in RAW 264.7 Mouse Cell Line. MyD88 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse MyD88 Monoclonal Antibody (Catalog # MAB3109) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MyD88
MyD88 was first identified as a myeloid differentiation primary response protein. It contains a cytoplasmic Toll/interleukin-1 receptor (TIR) domain and is classified as a member of the TIR superfamily. MyD88 is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.
  • Long Name:
    Myeloid Differentiation Primary Response Gene 88
  • Entrez Gene IDs:
    4615 (Human); 17874 (Mouse)
  • Alternate Names:
    MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cells.
    Authors: Jiang G, Sun D, Yang H, Lu Q, Kaplan H, Shao H
    J Leukoc Biol, 2014;95(4):599-607.
  2. Bacterial recognition by TLR7 in the lysosomes of conventional dendritic cells.
    Authors: Mancuso G, Gambuzza M, Midiri A, Biondo C, Papasergi S, Akira S, Teti G, Beninati C
    Nat. Immunol., 2009;10(6):587-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Cell and Tissue Staining Kits
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CTS017 5
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Rat IgG2A Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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