MyD88 in RAW 264.7 Mouse Cell Line.
MyD88 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse MyD88 Monoclonal Antibody (Catalog # MAB3109) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MyD88 was first identified as a myeloid differentiation primary
response protein. It contains a cytoplasmic Toll/interleukin-1 receptor
(TIR) domain and is classified as a member of the TIR superfamily.
MyD88 is expressed in many adult tissues including heart, kidney and
liver tissue and in monocyte, T, B, NK and dendritic cells.
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