Detection of Mouse NAC1 by Western Blot.
Western blot shows lysates of D3 mouse embryonic stem cell line, F9 mouse teratocarcinoma stem cells, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NAC1 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NAC1 in Mouse Embryo.
NAC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375) at a 1:80 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to developing primary sensory neurons in dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse NAC1 by Simple WesternTM.
Simple Western lane view shows lysate of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for NAC1 at approximately 69 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
NAC1 (Nucleus Accumbens-associated Protein 1) also known as NACC1, is a 527 amino acid (aa) BTB/POZ domain containing protein. Human NAC1 shares 86% and 85% aa sequence identity with mouse and rat NAC1, respectively. It has been shown to have a role in transcriptional regulation in both pluripotent stem cells and in the brain. Additionally, NAC1 expression is upregulated in many cancers and this expression has been suggested to correlate with increased aggressiveness in some cancers.
Nucleus Accumbens Associated 1
Entrez Gene IDs:
112939 (Human); 66830 (Mouse); 171454 (Rat)
BEND8; BTB (POZ) domain containing 14B; BTB/POZ domain-containing protein 14B; BTBD14B; BTBD30; NAC1; NAC1BEN domain containing 8; NAC-1FLJ37383; NACC1; nucleus accumbens associated 1, BEN and BTB (POZ) domain containing; nucleus accumbens-associated protein 1; transcriptional repressor NAC1
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