Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 809220
View all NCAM-1/CD56 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NCAM‑1/CD56
Leu20-Thr711
Accession # P13595

Specificity

Detects mouse NCAM‑1/CD56 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse NCAM‑1/CD56 APC‑conjugated Antibody

Detection of NCAM-1/CD56 antibody in Neuro-2A Mouse Cell Line antibody by Flow Cytometry.

Detection of NCAM‑1/CD56 in Neuro‑2A Mouse Cell Line by Flow Cytometry.

Neuro-2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB7820A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse NCAM‑1/CD56 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Neuro‑2A mouse neuroblastoma cell line

Reviewed Applications

Read 1 review rated 4 using FAB7820A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NCAM-1/CD56

NCAM-1 (Neural adhesion molecule-1; also CD56) is a 120-190 kDa glycoprotein member of the Ig Superfamily. It is expressed on multiple cell types, both in the embryo and adult. Here, it serves as both an adhesion molecule and a receptor for multiple ligands, including as FGFR, PDGF, GDNF and agrin. On the cell surface, it is a cis-oriented homodimer that can form homodimers in-trans with other cis-homodimers. In the embryo, NCAM-1 is polysialylated (PolySia), and shows a MW of 200-220 kDa in SDS-PAGE. This polysialylation reduces the ability of NCAM-1 to dimerize. Mature mouse NCAM-1 is a 1096 amino acid (aa) type I transmembrane (TM) protein (aa 20-1115). It possesses a 692 aa extracellular region (aa 20-711) and a 386 aa cytoplasmic domain. The extracellular region contains five consecutive C2-type Ig-like domains (aa 20-492) followed by two FN type-III domains (aa 497-692). Multiple splice variants exist. There is a 140 kDa TM variant that shows a deletion of aa 810-1076, and a 120 kDa variant that is GPI-linked and shows a 24 aa substitution for aa 702-1115. A third potential variant contains a five aa substitution for aa 601-1115. Over aa 20-711, mouse NCAM-1 shares 99% and 95% aa identity with rat and human NCAM-1, respectively.

Long Name

Neural Cell Adhesion Molecule

Alternate Names

CD56, NCAM1

Entrez Gene IDs

4684 (Human); 17967 (Mouse); 24586 (Rat)

Gene Symbol

NCAM1

UniProt

P13595

Additional NCAM-1/CD56 Products

Product Documents for Mouse NCAM‑1/CD56 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse NCAM‑1/CD56 APC‑conjugated Antibody

For research use only

Citations for Mouse NCAM‑1/CD56 APC‑conjugated Antibody

Customer Reviews for Mouse NCAM‑1/CD56 APC‑conjugated Antibody (1)

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  • Mouse NCAM-1/CD56 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Murine Splenocytes (C57B6)
    Species: Mouse
    Verified Customer | Posted 02/21/2019
    Splenocytes from EAE mice stained for CD56.
    Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and splenocytes were isolated when the animals were at the peak of the disease. Cells were stained for CD56, CD11c, CD11b, CD4, CD45 and CD95, plus for viability to exclude dead cells. CD56 staining is showed in viable esplenocytes.
    Mouse NCAM‑1/CD56 APC‑conjugated Antibody FAB7820A

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