Mouse NCAM-1/CD56 APC-conjugated Antibody

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Detection of NCAM‑1/CD56 in Neuro‑2A Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (7)
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Mouse NCAM-1/CD56 APC-conjugated Antibody Summary

Species Reactivity
Detects mouse NCAM‑1/CD56 in direct ELISAs.
Monoclonal Rat IgG2A Clone # 809220
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse NCAM‑1/CD56
Accession # P13595
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NCAM-1/CD56 antibody in Neuro-2A Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of NCAM‑1/CD56 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB7820A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NCAM-1/CD56

NCAM-1 (Neural adhesion molecule-1; also CD56) is a 120-190 kDa glycoprotein member of the Ig Superfamily. It is expressed on multiple cell types, both in the embryo and adult. Here, it serves as both an adhesion molecule and a receptor for multiple ligands, including as FGFR, PDGF, GDNF and agrin. On the cell surface, it is a cis-oriented homodimer that can form homodimers in-trans with other cis-homodimers. In the embryo, NCAM-1 is polysialylated (PolySia), and shows a MW of 200-220 kDa in SDS-PAGE. This polysialylation reduces the ability of NCAM-1 to dimerize. Mature mouse NCAM-1 is a 1096 amino acid (aa) type I transmembrane (TM) protein (aa 20-1115). It possesses a 692 aa extracellular region (aa 20-711) and a 386 aa cytoplasmic domain. The extracellular region contains five consecutive C2-type Ig-like domains (aa 20-492) followed by two FN type-III domains (aa 497-692). Multiple splice variants exist. There is a 140 kDa TM variant that shows a deletion of aa 810-1076, and a 120 kDa variant that is GPI-linked and shows a 24 aa substitution for aa 702-1115. A third potential variant contains a five aa substitution for aa 601-1115. Over aa 20-711, mouse NCAM-1 shares 99% and 95% aa identity with rat and human NCAM-1, respectively.

Long Name
Neural Cell Adhesion Molecule
Entrez Gene IDs
4684 (Human); 17967 (Mouse); 24586 (Rat)
Alternate Names
CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM

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Citations for Mouse NCAM-1/CD56 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. In vivo study of the immune response to bioengineered spider silk spheres
    Authors: T Deptuch, K Penderecka, M Kaczmarek, S Molenda, H Dams-Kozlo
    Scientific Reports, 2022-08-05;12(1):13480.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Authors: YT Shue, AP Drainas, NY Li, SM Pearsall, D Morgan, N Sinnott-Ar, SQ Hipkins, GL Coles, JS Lim, AE Oro, KL Simpson, C Dive, J Sage
    Nature Communications, 2022-05-16;13(1):2690.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Flow Cytometry
  3. STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells
    Authors: L Jin, Y Chen, DK Crossman, A Datta, T Vu, JA Mobley, MK Basu, M Scarduzio, H Wang, C Chang, PK Datta
    Nat Commun, 2020-11-23;11(1):5941.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Oncolytic virotherapy for small-cell lung cancer induces immune infiltration and prolongs survival
    Authors: P Kellish, D Shabashvil, MM Rahman, A Nawab, MV Guijarro, M Zhang, C Cao, N Moussatche, T Boyle, S Antonia, M Reinhard, C Hartzell, M Jantz, HJ Mehta, G McFadden, FJ Kaye, M Zajac-Kaye
    J. Clin. Invest., 2019-04-29;130(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CNS-derived extracellular vesicles from superoxide dismutase 1 (SOD1)G93A ALS mice originate from astrocytes and neurons and carry misfolded SOD1
    Authors: JM Silverman, D Christy, CC Shyu, KM Moon, S Fernando, Z Gidden, CM Cowan, Y Ban, RG Stacey, LI Grad, L McAlary, IR Mackenzie, LJ Foster, NR Cashman
    J. Biol. Chem., 2019-01-11;0(0):.
    Species: Mouse
    Sample Types: Microvesicles
    Applications: Flow Cytometry
  6. GDF10 is a signal for axonal sprouting and functional recovery after stroke.
    Authors: Li S, Nie E, Yin Y, Benowitz L, Tung S, Vinters H, Bahjat F, Stenzel-Poore M, Kawaguchi R, Coppola G, Carmichael S
    Nat Neurosci, 2015-10-26;18(12):1737-45.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  7. Neuronal transgene expression in dominant-negative SNARE mice.
    Authors: Fujita T, Chen M, Li B, Smith N, Peng W, Sun W, Toner M, Kress B, Wang L, Benraiss A, Takano T, Wang S, Nedergaard M
    J Neurosci, 2014-12-10;34(50):16594-604.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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