Mouse Netrin-1 Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Product Details
Citations (10)
Supplemental Products
Reviews (1)

Mouse Netrin-1 Antibody Summary

Species Reactivity
Detects mouse Netrin-1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant chicken (rch) Netrin‑1 and no cross-reactivity with rchNetrin-2, recombinant human Netrin-4, recombinant mouse (rm) Netrin-4, or rmNetrin‑G1a is observed.
Monoclonal Rat IgG2A Clone # 158936
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Netrin-1
Accession # AAC52971
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse Netrin‑1 (Catalog # 1109-N1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-1

Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma -chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin‑1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis,
Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1-7).

  1. Puschel, A. (1999) Mech. Dev. 83:65.
  2. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. Madison, R. et al. (2000) Exp. Neurology 161:563.
  5. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  6. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  7. Corset, V. et al. (2000) Nature 407:747.
Entrez Gene IDs
9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of

Product Datasheets

You must select a language.


Citations for Mouse Netrin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Allosteric potentiation of GABAA receptor single-channel conductance by netrin-1 during neuronal-excitation-induced inhibitory synaptic homeostasis
    Authors: ES Chan, Y Ge, YW So, YF Bai, L Liu, YT Wang
    Cell Reports, 2022-11-01;41(5):111584.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunodepletion
  2. Implication of Netrin-1 Gain of Expression in Canine Nodal Lymphoma
    Authors: A Tortereau, N Milhau, E Rhumy, M Castets, F Ponce, P Mehlen, T Marchal
    Veterinary sciences, 2022-09-10;9(9):.
    Species: Canine
    Sample Types: Whole Cells, Whole Tissue
    Applications: FLOW, Flow Cytometry, IHC
  3. Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury
    Authors: JT Chen, L Schmidt, C Schürger, MK Hankir, SM Krug, HL Rittner
    International Journal of Molecular Sciences, 2021-09-18;22(18):.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  4. Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity
    Authors: P Mehrpouya-, K Miranda, NP Singh, EE Zumbrun, M Nagarkatti, PS Nagarkatti
    J. Biol. Chem., 2019-03-25;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Mutations in the netrin-1 gene cause congenital mirror movements
    Authors: A Méneret, EA Franz, O Trouillard, TC Oliver, Y Zagar, SP Robertson, Q Welniarz, RJM Gardner, C Gallea, M Srour, C Depienne, CL Jasoni, C Dubacq, F Riant, JC Lamy, MP Morel, R Guérois, J Andreani, C Fouquet, M Doulazmi, M Vidailhet, GA Rouleau, A Brice, A Chédotal, I Dusart, E Roze, D Markie
    J. Clin. Invest., 2017-09-25;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance
    Authors: C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal
    Nature, 2017-04-26;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  7. Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response
    Authors: T Lahlali, ML Plissonnie, C Romero-Lóp, M Michelet, B Ducarouge, A Berzal-Her, F Zoulim, P Mehlen, R Parent
    Cell Mol Gastroenterol Hepatol, 2016-01-09;2(3):281-301.e9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014-03-02;20(4):377-84.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Neuronal guidance protein Netrin-1 induces differentiation in human embryonal carcinoma cells.
    Authors: Mancino M, Esposito C, Watanabe K, Nagaoka T, Gonzales M, Bianco C, Normanno N, Salomon DS, Strizzi L
    Cancer Res., 2009-02-17;69(5):1717-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Netrin-1 interacts with amyloid precursor protein and regulates amyloid-beta production.
    Authors: Lourenco FC, Galvan V, Fombonne J, Corset V, Llambi F, Muller U, Bredesen DE, Mehlen P
    Cell Death Differ., 2009-01-16;16(5):655-63.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Netrin-1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Mouse Netrin-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Mouse Netrin-1 Antibody
By Lolita Singh on 11/19/2020
Application: IHC Sample Tested: Dorsal root ganglion,Peripheral nerve,Spinal cord tissue Species: Mouse

Immunohistochemistry worked nicely with this antibody. I performed staining in transverse sections of C57BL/6 mouse DRGs, Spinal cord sections and longitudinal sections of sciatic nerve. I would recommend doing antigen retrieval using sodium citrate buffer at pH 6.0 at a temperature of 80-85C for 30 min in a hot water bath. For all mentioned tissues I PFA fixed it overnight then cut small sections of the spinal cord and cut the sciatic nerve into two (longitudinally), antigen retrieved them in a 12-well plate with wells filled with sodium citrate buffer and kept in a pre-heated water bath. Following this I left the samples in 30% sucrose for two nights at 4C and then embedded it in OCT followed by cryosectioning and normal IHC protocol. Worked beautifully for my samples following this protocol.