Mouse Netrin-1 Antibody Summary
Val22-Ala603
Accession # AAC52971
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Netrin-1
Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma -chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin‑1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis,
Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1-7).
- Puschel, A. (1999) Mech. Dev. 83:65.
- Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
- Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
- Madison, R. et al. (2000) Exp. Neurology 161:563.
- Srinivasan, K. et al. (2003) Dev. Cell 4:371.
- Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
- Corset, V. et al. (2000) Nature 407:747.
Product Datasheets
Citations for Mouse Netrin-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Allosteric potentiation of GABAA receptor single-channel conductance by netrin-1 during neuronal-excitation-induced inhibitory synaptic homeostasis
Authors: ES Chan, Y Ge, YW So, YF Bai, L Liu, YT Wang
Cell Reports, 2022-11-01;41(5):111584.
Species: Mouse
Sample Types: Whole Cells
Applications: Immunodepletion -
Implication of Netrin-1 Gain of Expression in Canine Nodal Lymphoma
Authors: A Tortereau, N Milhau, E Rhumy, M Castets, F Ponce, P Mehlen, T Marchal
Veterinary sciences, 2022-09-10;9(9):.
Species: Canine
Sample Types: Whole Cells, Whole Tissue
Applications: FLOW, Flow Cytometry, IHC -
Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury
Authors: JT Chen, L Schmidt, C Schürger, MK Hankir, SM Krug, HL Rittner
International Journal of Molecular Sciences, 2021-09-18;22(18):.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity
Authors: P Mehrpouya-, K Miranda, NP Singh, EE Zumbrun, M Nagarkatti, PS Nagarkatti
J. Biol. Chem., 2019-03-25;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Mutations in the netrin-1 gene cause congenital mirror movements
Authors: A Méneret, EA Franz, O Trouillard, TC Oliver, Y Zagar, SP Robertson, Q Welniarz, RJM Gardner, C Gallea, M Srour, C Depienne, CL Jasoni, C Dubacq, F Riant, JC Lamy, MP Morel, R Guérois, J Andreani, C Fouquet, M Doulazmi, M Vidailhet, GA Rouleau, A Brice, A Chédotal, I Dusart, E Roze, D Markie
J. Clin. Invest., 2017-09-25;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Floor-plate-derived netrin-1 is dispensable for commissural axon guidance
Authors: C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal
Nature, 2017-04-26;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response
Authors: T Lahlali, ML Plissonnie, C Romero-Lóp, M Michelet, B Ducarouge, A Berzal-Her, F Zoulim, P Mehlen, R Parent
Cell Mol Gastroenterol Hepatol, 2016-01-09;2(3):281-301.e9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
Nat Med, 2014-03-02;20(4):377-84.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neuronal guidance protein Netrin-1 induces differentiation in human embryonal carcinoma cells.
Authors: Mancino M, Esposito C, Watanabe K, Nagaoka T, Gonzales M, Bianco C, Normanno N, Salomon DS, Strizzi L
Cancer Res., 2009-02-17;69(5):1717-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Netrin-1 interacts with amyloid precursor protein and regulates amyloid-beta production.
Authors: Lourenco FC, Galvan V, Fombonne J, Corset V, Llambi F, Muller U, Bredesen DE, Mehlen P
Cell Death Differ., 2009-01-16;16(5):655-63.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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Reviews for Mouse Netrin-1 Antibody
Average Rating: 5 (Based on 1 Review)
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Immunohistochemistry worked nicely with this antibody. I performed staining in transverse sections of C57BL/6 mouse DRGs, Spinal cord sections and longitudinal sections of sciatic nerve. I would recommend doing antigen retrieval using sodium citrate buffer at pH 6.0 at a temperature of 80-85C for 30 min in a hot water bath. For all mentioned tissues I PFA fixed it overnight then cut small sections of the spinal cord and cut the sciatic nerve into two (longitudinally), antigen retrieved them in a 12-well plate with wells filled with sodium citrate buffer and kept in a pre-heated water bath. Following this I left the samples in 30% sucrose for two nights at 4C and then embedded it in OCT followed by cryosectioning and normal IHC protocol. Worked beautifully for my samples following this protocol.
