Mouse Netrin-4 Biotinylated Antibody

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Mouse Netrin-4 Biotinylated Antibody Summary

Species Reactivity
Detects mouse Netrin‑4 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human Netrin-4 is observed and less than 1% cross-reactivity with recombinant mouse Netrin-1, recombinant chicken Netrin-2, and recombinant mouse Netrin‑G1a is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Netrin-4
Accession # Q9JI33
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Netrin‑4 (Catalog # 1132-N4)
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-4

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins binds to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-4, also known as beta -Netrin, is synthesized as a 629 amino acid (aa) precursor that contains a 19 aa signal sequence, a 428 aalaminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 182 aa C domain rich in basic aa residues that serves as a heparin binding site. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Mouse Netrin-4 shares 31%, 29%, and 25% aa sequence identity with mouse Netrin-1, -3, and -G1a, respectively. It also shares 89% aa sequence identity with human Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1-7).

Entrez Gene IDs
59277 (Human); 57764 (Mouse)
Alternate Names
beta-Netrin; hepar-derived netrin-like protein; netrin 4; Netrin4; Netrin-4; NTN4; PRO3091

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