Mouse NKG2D/CD314 Antibody

Name: Mouse NKG2D/CD314 Antibody
Brand: R&D Systems
SKU: MAB1547-100
Rating: 5 (1 reviews)

Catalog #: MAB1547
13 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1547-SP
MAB1547-500
MAB1547-100

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Product Details

Citations (13)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NKG2D/CD314 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NKG2D in direct ELISAs and Western blots. Does not cross-react with recombinant human NKG2D.

Source

Monoclonal Rat IgG_2B Clone # 191004

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NKG2D
Phe94-Val232
Accession # O54709

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse NKG2D/CD314 Fc Chimera (Catalog # 139-NK)
under non-reducing conditions only

Flow Cytometry

2.5 µg/10⁶ cells

NK1.1⁺ mouse splenic NK cells

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.05-0.15 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated Recombinant Mouse Rae-1 gamma Fc Chimera to immobilized Recombinant Mouse NKG2D Fc Chimera (Catalog # 139‑NK) coated at 4 µg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2D/CD314

NKG2D, also known as CD314, is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

References

Bauer, S. et al. (1999) Science 285:727.
Wu, J. et al. (1999) Science 285:730.
Diefenbach, A. et al. (2001) Nature 413:165.
Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
NKG2D and its Ligands; www.RnDSystems.com.

Long Name

Natural Killer G2D

Entrez Gene IDs

22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)

Alternate Names

CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse NKG2D/CD314 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Combination of anti-CD25 antibody and poly I:C treatment in pregnant NOD mice may be used as "pregnancy-related" type 1 diabetes model
Authors: A Shimada, K Toda, I Inoue, T Yamada, Y Oikawa
Journal of diabetes investigation, 2022-05-26;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice
Authors: L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian
J. Cell. Mol. Med, 2017-03-09;0(0):.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization
HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing.
Authors: Ham H, Huynh W, Schoon R, Vale R, Billadeau D
J Immunol, 2015-03-11;194(8):3984-96.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
J Invest Dermatol, 2015-01-29;135(5):1311-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
NKG2D triggers cytotoxicity in murine epidermal gammadelta T cells via PI3K-dependent, Syk/ZAP70-independent signaling pathway.
Authors: Ibusuki A, Kawai K, Yoshida S, Uchida Y, Nitahara-Takeuchi A, Kuroki K, Kajikawa M, Ose T, Maenaka K, Kasahara M, Kanekura T
J Invest Dermatol, 2013-08-20;134(2):396-404.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling.
Authors: Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, Zeissig S, Wang J, Mandelboim O, Beauchemin N, Lanier L, Blumberg R
Eur J Immunol, 2013-06-18;43(9):2473-83.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
Mucosal Immunol, 2012-06-13;5(0):702.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection.
Authors: Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z
Infect. Immun., 2011-12-05;80(2):565-74.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization
Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties.
Authors: Slavuljica I, Busche A, Babic M, Mitrovic M, Gasparovic I, Cekinovic D, Markova Car E, Pernjak Pugel E, Cikovic A, Lisnic VJ, Britt WJ, Koszinowski U, Messerle M, Krmpotic A, Jonjic S
J. Clin. Invest., 2010-11-22;120(12):4532-45.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
Blood, 2009-02-06;113(24):6120-7.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D.
Authors: Takada A, Yoshida S, Kajikawa M, Miyatake Y, Tomaru U, Sakai M, Chiba H, Maenaka K, Kohda D, Fugo K, Kasahara M
J. Immunol., 2008-02-01;180(3):1678-85.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

Activation of immature hepatic NK cells as immunotherapy for liver metastatic disease.
Authors: Bahjat KS, Prell RA, Allen HE, Liu W, Lemmens EE, Leong ML, Portnoy DA, Dubensky TW, Brockstedt DG, Giedlin MA
J. Immunol., 2007-12-01;179(11):7376-84.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

T regulatory cells control numbers of NK cells and CD8alpha+ immature dendritic cells in the lymph node paracortex.
Authors: Giroux M, Yurchenko E, St-Pierre J, Piccirillo CA, Perreault C
J. Immunol., 2007-10-01;179(7):4492-502.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr