Mouse NKG2D/CD314 Antibody

Detection of NKG2D/CD314 by Flow Cytometry NK Require NKG2D to Control Early Virus Dissemination & for Optimal Cytotoxicity but Not Activation(A) B6 mice infected with 3,000 pfu ECTV. On day 5 PI, mice pulsed with BrdU for 3 h & their spleens analyzed by flow cytometry. Plots are gated on CD3ɛ− NK1.1+. Data correspond to pools of three mice from three individual experiments.(B) Increased viral titers of infected mice with NKG2D blockade in vivo. Intact B6 mice, B6 mice with NKG2D blockade, B6 mice depleted of NK, B6 mice depleted of T (treated with anti-CD4 + anti-CD8 mAbs), or depleted of T & with NKG2D blockade infected with 3,000 pfu ECTV & the viral titers in spleen determined on day 3 PI. Data are the average ± SD of three individual mice & are representative of two similar experiments.(C) The indicated mice infected with 3,000 pfu ECTV, & the NK responses in the D-LN determined at 2 d PI. Upper panel: Dot plot showing the proportion of NK (DX5+, CD3e−) in the D-LN of infected & control uninfected mice. Lower panel: GzB & IFN-gamma production by NK. correspond to the DX5+, CD3ɛ− gate of the upper panels. Data correspond to pools of three mice & are representative of three similar experiments.(D) NK purified from spleens (5 d PI) of ECTV-infected intact or NKG2D-blocked mice & stained as indicated. Filled histogram: isotype-matched control Ig; gray line: NKG2D-blocked mice; black line: intact mice.(E) NK purified from spleens of intact or NKG2D-blocked mice as indicated & used as effectors in a 4-h 51Cr release assay against the indicated targets. Data correspond to pools of three mice & are representative of three experiments.(F) As in (D), but the NK purified from untreated mice & a neutralizing anti-NKG2D mAb was added to the in vitro cytotoxicity assays, as indicated. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/18266471), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of NKG2D/CD314 by Flow Cytometry NK Require NKG2D to Control Early Virus Dissemination & for Optimal Cytotoxicity but Not Activation(A) B6 mice infected with 3,000 pfu ECTV. On day 5 PI, mice pulsed with BrdU for 3 h & their spleens analyzed by flow cytometry. Plots are gated on CD3ɛ− NK1.1+. Data correspond to pools of three mice from three individual experiments.(B) Increased viral titers of infected mice with NKG2D blockade in vivo. Intact B6 mice, B6 mice with NKG2D blockade, B6 mice depleted of NK, B6 mice depleted of T (treated with anti-CD4 + anti-CD8 mAbs), or depleted of T & with NKG2D blockade infected with 3,000 pfu ECTV & the viral titers in spleen determined on day 3 PI. Data are the average ± SD of three individual mice & are representative of two similar experiments.(C) The indicated mice infected with 3,000 pfu ECTV, & the NK responses in the D-LN determined at 2 d PI. Upper panel: Dot plot showing the proportion of NK (DX5+, CD3e−) in the D-LN of infected & control uninfected mice. Lower panel: GzB & IFN-gamma production by NK. correspond to the DX5+, CD3ɛ− gate of the upper panels. Data correspond to pools of three mice & are representative of three similar experiments.(D) NK purified from spleens (5 d PI) of ECTV-infected intact or NKG2D-blocked mice & stained as indicated. Filled histogram: isotype-matched control Ig; gray line: NKG2D-blocked mice; black line: intact mice.(E) NK purified from spleens of intact or NKG2D-blocked mice as indicated & used as effectors in a 4-h 51Cr release assay against the indicated targets. Data correspond to pools of three mice & are representative of three experiments.(F) As in (D), but the NK purified from untreated mice & a neutralizing anti-NKG2D mAb was added to the in vitro cytotoxicity assays, as indicated. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/18266471), licensed under a CC-BY license. Not internally tested by R&D Systems.