Mouse Nogo-B Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Nogo-B in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) Nogo-B and recombinant rat Nogo-A and no cross-reactivity with rhNogo-A is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 523808
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Nogo-B.
    Met1-Ile183 (predicted)
    Accession # Q99P72
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Nogo-B in Mouse Heart. Nogo-B was detected in immersion fixed frozen sections of adult mouse heart using Mouse Nogo-B Monoclonal Antibody (Catalog # MAB6596) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cardiac outflow tract. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo-B

Nogo-B, also known as ASY, is a 55 kDa splice form of Nogo-A/Reticulon-4 and is primarily expressed in vascular endothelial cells. Nogo-A contains a large N‑terminal cytoplasmic domain and a reticulon region that consists of two transmembrane segments with an intervening noncytoplasmic domain. Nogo-B lacks most of the N-terminal domain but retains the reticulon domain. It inhibits neointimal hyperplasia in the vasculature and is downregulated in atherosclerotic tissue and thoracic arterial aneurisms. Interaction of Nogo-B with NgBR promotes endothelial cell proliferation and migration. Within aa 1‑183, mouse Nogo-B shares 76% and 94% aa sequence identity with human and rat Nogo-B, respectively.

  • Long Name:
    Reticulon 4B
  • Entrez Gene IDs:
    57142 (Human); 68585 (Mouse); 83765 (Rat)
  • Alternate Names:
    NogoB; Nogo-B; RTN4B
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VisUCyte Antigen Retrieval Reagent-Basic

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Isotype Controls
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rat IgG Biotinylated Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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