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Mouse Osteopontin/OPN Antibody

Catalog #: AF808
124 Citations 8 Reviews Datasheet / COA / SDS
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AF808
AF808-SP

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Osteopontin/OPN in C2C12 Mouse Cell Line and Mouse Splenocytes.
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Citations (124)
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Mouse Osteopontin/OPN Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Osteopontin (OPN) in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R&D Systems, Catalog # 441-OP)
Leu17-Asn294 (Glu99Gly)
Accession # Q547B5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.30 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Mouse Osteopontin/OPN Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Osteopontin/OPN Biotinylated Antibody (Catalog # BAF808)

Standard: Recombinant Mouse Osteopontin/OPN Protein (Catalog # 441-OP)

Neutralization
Measured by its ability to neutralize Osteopontin/OPN-mediated adhesion of the HEK293 human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 2 µg/mL Recombinant Mouse Osteopontin/OPN.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Osteopontin/OPN antibody in C2C12 Mouse Cell Line and Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Osteopontin/OPN in C2C12 Mouse Cell Line and Mouse Splenocytes. Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Osteopontin/OPN antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

Osteopontin/OPN in Mouse Thymus. Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Mouse Osteopontin/OPN Antibody. View Larger

Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Mouse Osteopontin/OPN Antibody. Recombinant Mouse Osteopontin/OPN (441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Osteopontin/OPN (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The ND50 is typically 1-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteopontin/OPN

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

References
  1. Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21.
  2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

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Citations for Mouse Osteopontin/OPN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

124 Citations: Showing 1 - 10
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  1. Signalling by senescent melanocytes hyperactivates hair growth
    Authors: Wang, X;Ramos, R;Phan, AQ;Yamaga, K;Flesher, JL;Jiang, S;Oh, JW;Jin, S;Jahid, S;Kuan, CH;Nguyen, TK;Liang, HY;Shettigar, NU;Hou, R;Tran, KH;Nguyen, A;Vu, KN;Phung, JL;Ingal, JP;Levitt, KM;Cao, X;Liu, Y;Deng, Z;Taguchi, N;Scarfone, VM;Wang, G;Paolilli, KN;Wang, X;Guerrero-Juarez, CF;Davis, RT;Greenberg, EN;Ruiz-Vega, R;Vasudeva, P;Murad, R;Widyastuti, LHP;Lee, HL;McElwee, KJ;Gadeau, AP;Lawson, DA;Andersen, B;Mortazavi, A;Yu, Z;Nie, Q;Kunisada, T;Karin, M;Tuckermann, J;Esko, JD;Ganesan, AK;Li, J;Plikus, MV;
    Nature
    Species: Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  2. Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs
    Authors: E Yildiz, G El Alam, A Perino, A Jalil, PD Denechaud, K Huber, L Fajas, J Auwerx, G Sorrentino, K Schoonjans
    Elife, 2023-03-06;12(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Kupffer-cell-derived IL-6 is repurposed for hepatocyte dedifferentiation via activating progenitor genes from injury-specific enhancers
    Authors: L Li, L Cui, P Lin, Z Liu, S Bao, X Ma, H Nan, W Zhu, J Cen, Y Mao, X Ma, L Jiang, Y Nie, F Ginhoux, Y Li, H Li, L Hui
    Cell Stem Cell, 2023-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Perivascular cells induce microglial phagocytic states and synaptic engulfment via SPP1 in mouse models of Alzheimer's disease
    Authors: S De Scheppe, JZ Ge, G Crowley, LSS Ferreira, D Garceau, CE Toomey, D Sokolova, J Rueda-Carr, SH Shin, JS Kim, T Childs, T Lashley, JJ Burden, M Sasner, C Sala Frige, S Jung, S Hong
    Nature Neuroscience, 2023-02-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease
    Authors: Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor
    Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120.
    Species: Mouse
    Sample Types: Recombinant Protein, Whole Tissue
    Applications: Bioassay, IHC
  6. Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial Cells
    Authors: YS Song, N Jamali, CM Sorenson, N Sheibani
    Cells, 2023-01-16;12(2):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling
    Authors: Q Bai, X Wang, H Yan, L Wen, Z Zhou, Y Ye, Y Jing, Y Niu, L Wang, Z Zhang, J Su, T Chang, G Dou, Y Wang, J Sun
    Journal of personalized medicine, 2023-01-11;13(1):.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  8. Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation
    Authors: D Piekna-Prz, D Na, J Zhang, C Baker, JM Ashton, PM White
    Frontiers in Cellular Neuroscience, 2023-01-06;16(0):1096872.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Secreted phosphoprotein 1 slows neurodegeneration and rescues visual function in mouse models of aging and glaucoma
    Authors: S Li, TC Jakobs
    Cell Reports, 2022-12-27;41(13):111880.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Human Recombinant Lactoferrin Promotes Differentiation and Calcification on MC3T3-E1 Cells
    Authors: D Nagashima, Y Ishibashi, S Kawaguchi, M Furukawa, M Toho, M Ohno, T Nitto, N Izumo
    Pharmaceutics, 2022-12-25;15(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  11. Discoidin domain receptor 2 regulates aberrant mesenchymal lineage cell fate and matrix organization
    Authors: CA Pagani, AC Bancroft, RJ Tower, N Livingston, Y Sun, JY Hong, RN Kent, AL Strong, JH Nunez, JMR Medrano, N Patel, BA Nanes, KM Dean, Z Li, C Ge, BM Baker, AW James, SJ Weiss, RT Franceschi, B Levi
    Science Advances, 2022-12-21;8(51):eabq6152.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  12. Anti-osteopontin therapy leads to improved edema and infarct size in a murine model of ischemic stroke
    Authors: D Spitzer, T Puetz, M Armbrust, M Dunst, J Macas, F Croll, KH Plate, Y Reiss, S Liebner, PN Harter, S Guérit, K Devraj
    Scientific Reports, 2022-12-03;12(1):20925.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Cell Culture, In Vivo
  13. cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T�cells
    Authors: Y Fukushima, K Sakamoto, M Matsuda, Y Yoshikai, H Yagita, D Kitamura, M Chihara, N Minato, M Hattori
    Cell Reports, 2022-09-20;40(12):111373.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro
    Authors: T Patel, J Hammelman, S Aziz, S Jang, M Closser, TL Michaels, JA Blum, DK Gifford, H Wichterle
    Nature Communications, 2022-09-15;13(1):5427.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  15. Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia
    Authors: H Shi, Z Yin, Y Koronyo, DT Fuchs, J Sheyn, MR Davis, JW Wilson, MA Margeta, KM Pitts, S Herron, S Ikezu, T Ikezu, SL Graham, VK Gupta, KL Black, M Mirzaei, O Butovsky, M Koronyo-Ha
    Acta neuropathologica communications, 2022-09-08;10(1):136.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Cilostazol Attenuates AngII-Induced Cardiac Fibrosis in apoE Deficient Mice
    Authors: Y Hada, HA Uchida, R Umebayashi, M Yoshida, J Wada
    International Journal of Molecular Sciences, 2022-08-13;23(16):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. TET enzymes regulate skeletal development through increasing chromatin accessibility of RUNX2 target genes
    Authors: L Wang, X You, D Ruan, R Shao, HQ Dai, W Shen, GL Xu, W Liu, W Zou
    Nature Communications, 2022-08-11;13(1):4709.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  18. Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation
    Authors: S Pantasis, J Friemel, SM Brütsch, Z Hu, S Krautbauer, G Liebisch, J Dengjel, A Weber, S Werner, MR Bordoli
    Journal of Cell Science, 2022-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration
    Authors: S Lin, S Yin, J Shi, G Yang, X Wen, W Zhang, M Zhou, X Jiang
    Bioactive materials, 2022-03-24;18(0):116-127.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  20. Transcriptional and Distributional Profiling of Microglia in Retinal Angiomatous Proliferation
    Authors: A Schlecht, J Wolf, S Boneva, G Prinz, BM Braunger, P Wieghofer, H Agostini, G Schlunck, C Lange
    International Journal of Molecular Sciences, 2022-03-22;23(7):.
    Species: Mouse
    Sample Types: In Vivo
  21. Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure
    Authors: FZ Khamissi, L Ning, E Kefaloyian, H Dun, A Arthanaris, A Keller, JJ Atkinson, W Li, B Wong, S Dietmann, K Lavine, D Kreisel, A Herrlich
    Science Advances, 2022-02-25;8(8):eabm5900.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Induction of osteogenesis by bone-targeted Notch activation
    Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
    Elife, 2022-02-04;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Secreted osteopontin from CD4+ T�cells limits acute graft-versus-host disease
    Authors: N Aggarwal, ME Deerhake, D DiPalma, SK Shahi, MR Gaggioli, AK Mangalam, ML Shinohara
    Cell Reports, 2021-12-28;37(13):110170.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  24. STAT3 is critical for skeletal development and bone homeostasis by regulating osteogenesis
    Authors: S Zhou, Q Dai, X Huang, A Jin, Y Yang, X Gong, H Xu, X Gao, L Jiang
    Nature Communications, 2021-11-25;12(1):6891.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Osteopontin Deficiency Ameliorates Prostatic Fibrosis and Inflammation
    Authors: P Popovics, A Jain, KO Skalitzky, E Schroeder, H Ruetten, M Cadena, KS Uchtmann, CM Vezina, WA Ricke
    International Journal of Molecular Sciences, 2021-11-18;22(22):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells
    Authors: S Nonoyama, T Karakida, R Chiba-Ohku, R Yamamoto, Y Ujiie, T Nagano, Y Yamakoshi, K Gomi
    Cells, 2021-11-04;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Serotonin transporter-mediated molecular axis regulates regional retinal ganglion cell vulnerability and axon regeneration after nerve injury
    Authors: R Kingston, D Amin, S Misra, JM Gross, T Kuwajima
    PloS Genetics, 2021-11-04;17(11):e1009885.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Creation of X-linked Alport syndrome rat model with Col4a5 deficiency
    Authors: M Namba, T Kobayashi, M Kohno, T Koyano, T Hirose, M Fukushima, M Matsuyama
    Scientific Reports, 2021-10-21;11(1):20836.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  29. IER2-induced senescence drives melanoma invasion through osteopontin
    Authors: L Kyjacova, R Saup, K Rönsch, S Wallbaum, S Dukowic-Sc, A Foss, SD Scherer, M Rothley, A Neeb, N Grau, W Thiele, S Thaler, N Cremers, C Sticht, N Gretz, BK Garvalov, J Utikal, JP Sleeman
    Oncogene, 2021-10-05;0(0):.
    Species: Mouse
    Sample Types: CellLysates, Whole Tissue
    Applications: IHC, Western Blot
  30. Differential Response of M�ller Cells and Microglia in a Mouse Retinal Detachment Model and Its Implications in Detached and Non-Detached Regions
    Authors: SH Lee, YS Park, SS Paik, IB Kim
    Cells, 2021-08-03;10(8):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation
    Authors: L Cordero-Es, AM Dowbaj, TN Kohler, B Strauss, O Sarlidou, G Belenguer, C Pacini, NP Martins, R Dobie, JR Wilson-Kan, R Butler, N Prior, P Serup, F Jug, NC Henderson, F Hollfelder, M Huch
    Cell Stem Cell, 2021-08-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts
    Authors: J Sun, DY Shin, M Eiseman, AR Yallowitz, N Li, S Lalani, Z Li, M Cung, S Bok, S Debnath, SJ Marquez, TE White, AG Khan, IC Lorenz, JH Shim, FS Lee, R Xu, MB Greenblatt
    Nature Communications, 2021-07-29;12(1):4611.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  33. Regional specialization and fate specification of bone stromal cells in skeletal development
    Authors: KK Sivaraj, HW Jeong, B Dharmaling, D Zeuschner, S Adams, M Potente, RH Adams
    Cell Reports, 2021-07-13;36(2):109352.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics
    Authors: TA Jan, Y Eltawil, AH Ling, L Chen, DC Ellwanger, S Heller, AG Cheng
    Cell Reports, 2021-07-13;36(2):109358.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC, RNAScope
  35. EMX2-GPR156-G&alphai reverses hair cell orientation in mechanosensory epithelia
    Authors: KS Kindt, A Akturk, A Jarysta, M Day, A Beirl, M Flonard, B Tarchini
    Nature Communications, 2021-05-17;12(1):2861.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. MyD88 in myofibroblasts enhances colitis-associated tumorigenesis via promoting macrophage M2 polarization
    Authors: Q Yuan, J Gu, J Zhang, S Liu, Q Wang, T Tian, Z Chen, J Zhang
    Cell Reports, 2021-02-02;34(5):108724.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  37. Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility
    Authors: L Yu, Y Zheng, BJ Liu, MH Kang, JC Millar, DJ Rhee
    PLoS ONE, 2020-11-04;15(11):e0241294.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  38. Microglia-organized scar-free spinal cord repair in neonatal mice
    Authors: Y Li, X He, R Kawaguchi, Y Zhang, Q Wang, A Monavarfes, Z Yang, B Chen, Z Shi, H Meng, S Zhou, J Zhu, A Jacobi, V Swarup, PG Popovich, DH Geschwind, Z He
    Nature, 2020-10-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. Ectopic bone formation and systemic bone loss in a transmembrane TNF-driven model of human spondyloarthritis
    Authors: E Christodou, C Geka, L Ntari, K Kranidioti, E Argyropoul, F Meier, M Armaka, I Mourouzis, C Pantos, M Rouchota, G Loudos, MC Denis, N Karagianni, G Kollias
    Arthritis Res Ther, 2020-10-06;22(1):232.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  40. Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection
    Authors: FJ Mahmud, Y Du, E Greif, T Boucher, RF Dannals, WB Mathews, MG Pomper, P Sysa-Shah, KA Metcalf Pa, C Lyons, B Carlson, M Chacona, AM Brown
    Journal of Neuroinflammation, 2020-09-17;17(1):273.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  41. Loss of Osteopontin Expression Reduces HSV-1-Induced Corneal Opacity
    Authors: A Filiberti, GB Gmyrek, ML Montgomery, R Sallack, DJJ Carr
    Invest. Ophthalmol. Vis. Sci., 2020-08-03;61(10):24.
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  42. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
    Authors: J Falero-Per, N Sheibani, CM Sorenson
    PLoS ONE, 2020-05-04;15(5):e0232779.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  43. Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-?v&beta6 integrin in liver fibrosis
    Authors: T Xu, Z Lu, Z Xiao, F Liu, Y Chen, Z Wang, S Zhu, Y Song
    Cell Death Dis, 2020-03-23;11(3):199.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  44. Hox genes maintain critical roles in the adult skeleton
    Authors: JY Song, KM Pineault, JM Dones, RT Raines, DM Wellik
    Proc. Natl. Acad. Sci. U.S.A., 2020-03-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  45. Activating Transcription Factor 3 (ATF3) Protects Retinal Ganglion Cells and Promotes Functional Preservation After Optic Nerve Crush
    Authors: C Kole, B Brommer, N Nakaya, M Sengupta, L Bonet-Ponc, T Zhao, C Wang, W Li, Z He, S Tomarev
    Invest. Ophthalmol. Vis. Sci., 2020-02-07;61(2):31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  46. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells
    Authors: DJ Fu, L Wang, FK Chouairi, IM Rose, DA Abetov, AD Miller, RJ Yamulla, JC Schimenti, A Flesken-Ni, AY Nikitin
    Nat Commun, 2020-01-03;11(1):84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  47. Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury
    Authors: D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi
    Sci Rep, 2019-11-13;9(1):16736.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  48. In Vivo Activation and Pro-Fibrotic Function of NF-kappaB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon Nanotubes
    Authors: J Dong, Q Ma
    Front Pharmacol, 2019-10-02;10(0):1140.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  49. ATRQ β-001 Vaccine Prevents Experimental Abdominal Aortic Aneurysms
    Authors: H Zhang, M Liao, M Cao, Z Qiu, X Yan, Y Zhou, H Wu, Y Wang, J Zheng, J Ding, M Wang, Y Liao, X Chen
    J Am Heart Assoc, 2019-09-12;8(18):e012341.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  50. Survival of Alpha and Intrinsically Photosensitive Retinal Ganglion Cells in NMDA-Induced Neurotoxicity and a Mouse Model of Normal Tension Glaucoma
    Authors: S Honda, K Namekata, A Kimura, X Guo, C Harada, A Murakami, A Matsuda, T Harada
    Invest. Ophthalmol. Vis. Sci., 2019-09-03;60(12):3696-3707.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  51. Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis
    Authors: D van de Vle, J Demmers, XX Nguyen, Y Campos, E Machado, I Annunziata, H Hu, E Gomero, X Qiu, A Bongiovann, CA Feghali-Bo, A d'Azzo
    Sci Adv, 2019-07-17;5(7):eaav3270.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  52. Atoh1 Directs Regeneration and Functional Recovery of the Mature Mouse Vestibular System
    Authors: ZN Sayyid, T Wang, L Chen, SM Jones, AG Cheng
    Cell Rep, 2019-07-09;28(2):312-324.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  53. Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis
    Authors: E Quilichini, M Fabre, T Dirami, A Stedman, M De Vas, O Ozguc, RC Pasek, S Cereghini, L Morillon, C Guerra, A Couvelard, M Gannon, C Haumaitre
    Cell Mol Gastroenterol Hepatol, 2019-06-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  54. Single-Cell Analysis of the Liver Epithelium Reveals Dynamic Heterogeneity and an Essential Role for YAP in Homeostasis and Regeneration
    Authors: BJ Pepe-Moone, MT Dill, A Alemany, J Ordovas-Mo, Y Matsushita, A Rao, A Sen, M Miyazaki, S Anakk, PA Dawson, N Ono, AK Shalek, A van Oudena, FD Camargo
    Cell Stem Cell, 2019-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  55. Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation
    Authors: SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy
    Nat. Cell Biol., 2019-04-01;21(4):430-441.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  56. Pulmonary arterial remodelling by deficiency of peroxisome proliferator-activated receptor-? in murine vascular smooth muscle cells occurs independently of obesity-related pulmonary hypertension
    Authors: E Caglayan, M Trappiel, A Behringer, EM Berghausen, M Odenthal, E Wellnhofer, K Kappert
    Respir. Res., 2019-02-27;20(1):42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  57. Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells
    Authors: DK Wasgewatte, EJ Mackie, CN Pagel
    Skelet Muscle, 2019-02-26;9(1):6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  58. Osteopontin in the host response to Leishmania amazonensis
    Authors: E Giraud, E Rouault, L Fiette, JH Colle, D Smirlis, E Melanitou
    BMC Microbiol., 2019-02-08;19(1):32.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  59. N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells
    Authors: M Zhao, F Tao, A Venkatrama, Z Li, SE Smith, J Unruh, S Chen, C Ward, P Qian, JM Perry, H Marshall, J Wang, XC He, L Li
    Cell Rep, 2019-01-15;26(3):652-669.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  60. Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients
    Authors: H Hu, Z Liu, C Liu
    Oncol Lett, 2019-01-07;17(3):2788-2794.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  61. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
    Authors: KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill
    Elife, 2018-12-27;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  62. H3K36 trimethylation mediated by SETD2 regulates the fate of bone marrow mesenchymal stem cells
    Authors: L Wang, N Niu, L Li, R Shao, H Ouyang, W Zou
    PLoS Biol., 2018-11-13;16(11):e2006522.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  63. Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression
    Authors: MN Lee, HS Hwang, SH Oh, A Roshanzade, JW Kim, JH Song, ES Kim, JT Koh
    Exp. Mol. Med., 2018-11-05;50(11):142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  64. Molecular fingerprinting of On-Off direction selective retinal ganglion cells across species and relevance to primate visual circuits
    Authors: OS Dhande, BK Stafford, K Franke, R El-Danaf, KA Percival, AH Phan, P Li, BJ Hansen, PL Nguyen, P Berens, WR Taylor, E Callaway, T Euler, AD Huberman
    J. Neurosci., 2018-10-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  65. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
    Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
    PLoS ONE, 2018-10-29;13(10):e0206756.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  66. Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration
    Authors: HA Himburg, CM Termini, L Schlussel, J Kan, M Li, L Zhao, T Fang, JP Sasine, VY Chang, JP Chute
    Cell Stem Cell, 2018-08-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  67. Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
    Authors: Q Zeng, X Luo, M Han, W Liu, H Li
    EBioMedicine, 2018-06-07;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  68. Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation
    Authors: AM D'Souza, Y Jiang, A Cast, L Valanejad, M Wright, K Lewis, M Kumbaji, S Shah, D Smithrud, R Karns, S Shin, N Timchenko
    Cell Mol Gastroenterol Hepatol, 2018-05-24;6(3):239-255.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  69. Metabolic modulation of acetaminophen-induced hepatotoxicity by osteopontin
    Authors: Y Wen, C Wang, J Gu, C Yu, K Wang, X Sun, Y Sun, H Wu, Y Tong, Q Xia, X Kong
    Cell. Mol. Immunol., 2018-05-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  70. HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis
    Authors: C Hernandez, P Huebener, JP Pradere, DJ Antoine, RA Friedman, RF Schwabe
    J. Clin. Invest., 2018-05-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  71. Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Authors: KH Kim, SH Kim, DH Han, YS Jo, YH Lee, MS Lee
    Sci Rep, 2018-05-01;8(1):6789.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  72. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
    Authors: J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes
    Nat. Neurosci., 2018-04-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  73. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits
    Authors: W Ding, K Yousefi, S Goncalves, BJ Goldstein, AL Sabater, A Kloosterbo, P Ritter, G Lambert, AJ Mendez, LA Shehadeh
    JCI Insight, 2018-03-22;3(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  74. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
    Authors: T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi
    Front Immunol, 2018-03-06;9(0):334.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  75. Cardiac macrophages promote diastolic dysfunction
    Authors: M Hulsmans, HB Sager, JD Roh, M Valero-Muñ, NE Houstis, Y Iwamoto, Y Sun, RM Wilson, G Wojtkiewic, B Tricot, MT Osborne, J Hung, C Vinegoni, K Naxerova, DE Sosnovik, MR Zile, AD Bradshaw, R Liao, A Tawakol, R Weissleder, A Rosenzweig, FK Swirski, F Sam, M Nahrendorf
    J. Exp. Med., 2018-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  76. Type 2 diabetes impairs the ability of skeletal muscle pericytes to augment postischemic neovascularization in db/db mice
    Authors: KL Hayes, LM Messina, LM Schwartz, J Yan, AS Burnside, S Witkowski
    Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  77. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  78. Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin, TGF-?1, IL-13 and oxidative stress
    Authors: VCA de Souza, TA Pereira, VW Teixeira, H Carvalho, MCAB de Castro, CG D'assunção, AF de Barros, CL Carvalho, VMB de Lorena, VMA Costa, ÁAC Teixeira, RCBQ Figueiredo, SA de Oliveir
    World J. Gastroenterol., 2017-07-28;23(28):5146-5157.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  79. Four alpha ganglion cell types in mouse retina: Function, structure, and molecular signatures
    Authors: B Krieger, M Qiao, DL Rousso, JR Sanes, M Meister
    PLoS ONE, 2017-07-28;12(7):e0180091.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  80. Sox11 Expression Promotes Regeneration of Some Retinal Ganglion Cell Types but Kills Others
    Authors: MW Norsworthy, F Bei, R Kawaguchi, Q Wang, NM Tran, Y Li, B Brommer, Y Zhang, C Wang, JR Sanes, G Coppola, Z He
    Neuron, 2017-06-21;94(6):1112-1120.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  81. Monocyte Chemotactic Protein-1-Interleukin-6-Osteopontin Pathway of Intra-Aneurysmal Tissue Healing
    Authors: K Hosaka, K Rojas, HZ Fazal, MB Schneider, J Shores, V Federico, M McCord, L Lin, B Hoh
    Stroke, 2017-03-14;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  82. Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells
    Authors: N Guidi, M Sacma, L Ständker, K Soller, G Marka, K Eiwen, JM Weiss, F Kirchhoff, T Weil, JA Cancelas, MC Florian, H Geiger
    EMBO J, 2017-03-02;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  83. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017-02-20;19(3):189-201.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  84. Dentin Sialoprotein is a Novel Substrate of Matrix Metalloproteinase 9 in vitro and in vivo
    Authors: G Yuan, L Chen, J Feng, G Yang, Q Ni, X Xu, C Wan, M Lindsey, KJ Donly, M MacDougall, Z Chen, S Chen
    Sci Rep, 2017-02-14;7(0):42449.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  85. Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway
    Authors: CH Chen, YM Sue, CY Cheng, YC Chen, CT Liu, YH Hsu, PA Hwang, NJ Huang, TH Chen
    Sci Rep, 2017-01-18;7(0):40183.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  86. Disease-modifying effects of orally bioavailable NF-?B inhibitors in dystrophin-deficient muscle
    JCI Insight, 2016-12-22;1(21):e90341.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  87. Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9
    Authors: Yuta Morisaki
    Sci Rep, 2016-06-06;6(0):27354.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  88. The P2X4 receptor is required for neuroprotection via ischemic preconditioning
    Sci Rep, 2016-05-13;6(0):25893.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  89. Alternatively activated macrophages determine repair of the infarcted adult murine heart
    J Clin Invest, 2016-05-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  90. Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response
    Authors: K Zhao, M Zhang, L Zhang, P Wang, G Song, B Liu, H Wu, Z Yin, C Gao
    Sci Rep, 2016-03-30;6(0):23771.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Neutralization
  91. Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis
    Authors: F Wirsdörfer, S de Leve, F Cappuccini, T Eldh, AV Meyer, E Gau, LF Thompson, NY Chen, H Karmouty-Q, U Fischer, M Kasper, D Klein, JW Ritchey, MR Blackburn, AM Westendorf, M Stuschke, V Jendrossek
    Cancer Res., 2016-02-26;76(10):3045-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  92. Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.
    Authors: Mu X, Espanol-Suner R, Mederacke I, Affo S, Manco R, Sempoux C, Lemaigre F, Adili A, Yuan D, Weber A, Unger K, Heikenwalder M, Leclercq I, Schwabe R
    J Clin Invest, 2015-09-08;125(10):3891-903.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  93. Cutting edge: Role of osteopontin and integrin alphav in T cell-mediated anti-inflammatory responses in endotoxemia.
    Authors: Inoue M, Shinohara M
    J Immunol, 2015-05-13;194(12):5595-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates, In Vivo
    Applications: ELISA Development (Capture), Neutralization
  94. Identification of therapeutic targets for glioblastoma by network analysis.
    Authors: Friedmann-Morvinski D, Bhargava V, Gupta S, Verma I, Subramaniam S
    Oncogene, 2015-05-11;35(5):608-20.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  95. Osteopontin depletion decreases inflammation and gastric epithelial proliferation during Helicobacter pylori infection in mice.
    Authors: Park J, Lee S, Go D, Kim H, Kwon H, Kim D
    Lab Invest, 2015-04-13;95(6):660-71.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  96. Loss of JUNB/AP-1 promotes invasive prostate cancer.
    Authors: Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E
    Cell Death Differ, 2014-12-19;22(4):574-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  97. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2014-11-24;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  98. PGC-1alpha induces SPP1 to activate macrophages and orchestrate functional angiogenesis in skeletal muscle.
    Authors: Rowe G, Raghuram S, Jang C, Nagy J, Patten I, Goyal A, Chan M, Liu L, Jiang A, Spokes K, Beeler D, Dvorak H, Aird W, Arany Z
    Circ Res, 2014-07-09;115(5):504-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  99. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014-03-12;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  100. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
    Authors: Kusumbe A, Ramasamy S, Adams R
    Nature, 2014-03-12;507(7492):323-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  101. Colony-stimulating factor-1 signaling suppresses renal crystal formation.
    Authors: Taguchi K, Okada A, Kitamura H, Yasui T, Naiki T, Hamamoto S, Ando R, Mizuno K, Kawai N, Tozawa K, Asano K, Tanaka M, Miyoshi I, Kohri K
    J Am Soc Nephrol, 2014-02-27;25(8):1680-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  102. Osteopontin expression by CD103- dendritic cells drives intestinal inflammation.
    Authors: Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes D, Panoutsakopoulou V
    Proc Natl Acad Sci U S A, 2014-02-18;111(9):E856-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  103. An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells.
    Authors: Chen Q, Shou P, Zhang L, Xu C, Zheng C, Han Y, Li W, Huang Y, Zhang X, Shao C, Roberts A, Rabson A, Ren G, Zhang Y, Wang Y, Denhardt D, Shi Y
    Stem Cells, 2014-02-01;32(2):327-37.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  104. Hydrogen peroxide regulates osteopontin expression through activation of transcriptional and translational pathways.
    Authors: Lyle A, Remus E, Fan A, Lassegue B, Walter G, Kiyosue A, Griendling K, Taylor W
    J Biol Chem, 2013-11-18;289(1):275-85.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  105. Impaired hepatocyte maturation, abnormal expression of biliary transcription factors and liver fibrosis in C/EBPalpha(Cebpa)-knockout mice.
    Authors: Akai Y, Oitate T, Koike T, Shiojiri N
    Histol Histopathol, 2013-07-18;29(1):107-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  106. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-beta signaling pathways.
    Authors: Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N
    J Cell Sci, 2013-02-15;126(0):1392-405.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  107. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments.
    Authors: Park S, Wolfram P, Canty K, Harley B, Nombela-Arrieta C, Pivarnik G, Manis J, Beggs H, Silberstein L
    J Immunol, 2012-12-21;190(3):1094-102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  108. Identification of a clonally expanding haematopoietic compartment in bone marrow.
    Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R
    EMBO J, 2012-11-27;32(2):219-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  109. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012-08-29;82(12):1261-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  110. Repression of osteocyte Wnt/β-catenin signaling is an early event in the progression of renal osteodystrophy.
    Authors: Sabbagh Y, Graciolli FG, O'Brien S, Tang W, Dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC
    J. Bone Miner. Res., 2012-08-01;27(8):1757-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  111. Cellular basis of tissue regeneration by omentum.
    Authors: Shah S, Lowery E, Braun RK
    PLoS ONE, 2012-06-06;7(6):e38368.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  112. The critical role of agrin in the hematopoietic stem cell niche.
    Authors: Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A
    Blood, 2011-06-07;118(10):2733-42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  113. Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance.
    Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM
    Diabetes, 2010-01-27;59(4):935-46.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  114. Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
    Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
    FASEB J., 2009-08-31;24(1):70-80.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  115. Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse.
    Authors: Takanashi M, Oikawa K, Sudo K, Tanaka M, Fujita K, Ishikawa A, Nakae S, Kaspar RL, Matsuzaki M, Kudo M, Kuroda M
    Gene Ther., 2009-05-28;16(8):982-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  116. Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin.
    Authors: El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM
    Kidney Int., 2009-03-25;75(12):1297-307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  117. Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis.
    Authors: Murugaiyan G, Mittal A, Weiner HL
    J. Immunol., 2008-12-01;181(11):7480-8.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo
    Applications: Neutralization, Western Blot
  118. Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells.
    Authors: Terashima Y, Shimabukuro Y, Terashima H, Ozasa M, Terakura M, Ikezawa K, Hashikawa T, Takedachi M, Oohara H, Yamada S, Murakami S
    J. Cell. Physiol., 2008-09-01;216(3):640-50.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  119. In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression.
    Authors: Marcondes MC, Poling M, Watry DD, Hall D, Fox HS
    Cell. Immunol., 2008-08-03;254(1):56-62.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  120. Polyomavirus middle T antigen induces the transcription of osteopontin, a gene important for the migration of transformed cells.
    Authors: Whalen KA, Weber GF, Benjamin TL, Schaffhausen BS
    J. Virol., 2008-03-12;82(10):4946-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  121. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets.
    Authors: Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V
    Nat. Med., 2007-04-15;13(5):570-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  122. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
    Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
    Eur. Respir. J., 2006-04-26;28(2):275-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  123. Aggregation of embryonic stem cells induces Nanog repression and primitive endoderm differentiation.
    Authors: Hamazaki T, Oka M, Yamanaka S, Terada N
    J. Cell. Sci., 2004-10-19;117(0):5681-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  124. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.
    Authors: Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C
    Arthritis Rheum., 2004-08-01;50(8):2685-94.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

FAQs

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Isotype Controls

Normal Goat IgG Control

AB-108-C
377 Citations
20 Reviews
1 Image
Ctrl

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
3 Reviews

Secondary Antibodies

Chicken Anti-Goat IgG Biotinylated Antibody

BAF019
WB

Donkey Anti-Goat IgG Antibody

AF109
8 Citations
WB

Donkey Anti-Goat IgG Biotinylated Antibody

BAF109
3 Citations
1 Review
WB

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

NL003
8 Citations
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

NL001
28 Citations
2 Reviews
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

NL002
5 Citations
Flow,IHC,ICC

Goat IgG (H+L) APC-conjugated Antibody

F0108
14 Citations
1 Image
Flow

Goat IgG (H+L) Antibody

R-401-C-ABS
WB,ELISA

Goat IgG (H+L) Fluorescein-conjugated Antibody

F0109
3 Citations
1 Image
Flow

Goat IgG (H+L) PE-conjugated Antibody

F0107
11 Citations
2 Reviews
1 Image
Flow

Goat IgG (H+L) PerCP-conjugated Antibody

F0124
Flow

Goat IgG HRP-conjugated Antibody

HAF109
51 Citations
4 Reviews
2 Images
WB,Simple Western

Goat IgG HRP-conjugated Antibody

HAF017
58 Citations
10 Reviews
1 Image
WB

Goat IgG Horseradish Peroxidase-conjugated Antibody

HAF019
21 Citations
2 Images
WB,IHC

Goat IgG VisUCyte HRP Polymer

VC004
8 Citations
1 Image
IHC

Rabbit Anti-Goat IgG Biotinylated Antibody

BAF017
2 Citations
1 Review
WB

Reviews for Mouse Osteopontin/OPN Antibody

Average Rating: 4.3 (Based on 8 Reviews)

5 Star
62.5%
4 Star
25%
3 Star
0%
2 Star
0%
1 Star
12.5%

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Mouse Osteopontin/OPN Antibody
By Anonymous on 09/18/2022
Application: Flow Sample Tested: Lung cancer tissue Species: Mouse

Try it for flow cytometry

Flow Cytometry Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 07/14/2022
Application: ICC/IF Sample Tested: Adult pancreas Species: Mouse

Osteopontin (in white) was stained 1:200 on cryostat sections together with CD324-AF488 (in green). Ducts are clearly stained as expected.

Immunocytochemistry/Immunofluorescence Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Loïc Joannes on 07/02/2021
Application: WB Sample Tested: Cancer cell lysates,Cell Culture Media Species: Mouse

Antibody reacts with BSA and some of the ladder proteins from Biorad. Unusable for WB since BSA size is too close from the OSTP size.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.*** Update from Tech Support Team: Possible customer error, no response from customer.***

Western Blot Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 11/22/2020
Application: Func Sample Tested: Plasma Species: Mouse
Functional Assay Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 06/17/2020
Application: IHC Sample Tested: Liver tissue Species: Mouse
Immunohistochemistry Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Mingxia Zhou on 02/16/2020
Application: WB Sample Tested: A20 mouse B cell lymphoma cell line Species: Mouse
Western Blot Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Christian Schuerch on 08/31/2018
Application: ICC/IF Sample Tested: bone marrow Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 04/26/2017
Application: ICC/IF Sample Tested: Cell Culture Samples Species: Rat