Mouse Osteopontin/OPN Antibody AF808: R&D Systems

Mouse Osteopontin/OPN Antibody

(44 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Osteopontin (OPN) in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human OPN is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R&D Systems, Catalog # 441-OP)
    Leu17-Asn294 (Glu99Gly)
    Accession # Q547B5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.30 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse Osteopontin/OPN Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Osteopontin/OPN Antibody (Catalog # AF808)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Osteopontin/OPN Biotinylated Antibody (Catalog # BAF808)
  • ELISA Standard
     
    Recombinant Mouse Osteopontin (OPN) Protein (Catalog # 441-OP)
  • Neutralization
    Measured by its ability to neutralize Osteopontin/OPN-mediated adhesion of the HEK293 human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 2 µg/mL Recombinant Mouse Osteopontin/OPN.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Mouse Osteopontin/OPN Antibody. Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Osteopontin/OPN (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The ND50 is typically 1‑3 µg/mL.
Immunohistochemistry
Osteopontin/OPN in Mouse Thymus. Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteopontin/OPN

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

  • References:
    1. Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21.
    2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
    3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.
  • Long Name:
    Secreted Phosphoprotein 1 [BNSP]
  • Entrez Gene IDs:
    6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
  • Alternate Names:
    BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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Applications
Sample Type
  1. Dentin Sialoprotein is a Novel Substrate of Matrix Metalloproteinase 9 in vitro and in vivo
    Authors: G Yuan, L Chen, J Feng, G Yang, Q Ni, X Xu, C Wan, M Lindsey, KJ Donly, M MacDougall, Z Chen, S Chen
    Sci Rep, 2017;7(0):42449.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway
    Authors: CH Chen, YM Sue, CY Cheng, YC Chen, CT Liu, YH Hsu, PA Hwang, NJ Huang, TH Chen
    Sci Rep, 2017;7(0):40183.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  3. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017;19(3):189-201.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin, TGF-?1, IL-13 and oxidative stress
    Authors: VCA de Souza, TA Pereira, VW Teixeira, H Carvalho, MCAB de Castro, CG D'assunção, AF de Barros, CL Carvalho, VMB de Lorena, VMA Costa, ÁAC Teixeira, RCBQ Figueiredo, SA de Oliveir
    World J. Gastroenterol., 2017;23(28):5146-5157.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  5. Four alpha ganglion cell types in mouse retina: Function, structure, and molecular signatures
    Authors: B Krieger, M Qiao, DL Rousso, JR Sanes, M Meister
    PLoS ONE, 2017;12(7):e0180091.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  6. The P2X4 receptor is required for neuroprotection via ischemic preconditioning
    Sci Rep, 2016;6(0):25893.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9
    Authors: Yuta Morisaki
    Sci Rep, 2016;6(0):27354.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  8. Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response
    Authors: K Zhao, M Zhang, L Zhang, P Wang, G Song, B Liu, H Wu, Z Yin, C Gao
    Sci Rep, 2016;6(0):23771.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: Neut
  9. Disease-modifying effects of orally bioavailable NF-?B inhibitors in dystrophin-deficient muscle
    JCI Insight, 2016;1(21):e90341.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. Alternatively activated macrophages determine repair of the infarcted adult murine heart
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  11. Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis
    Authors: F Wirsdörfer, S de Leve, F Cappuccini, T Eldh, AV Meyer, E Gau, LF Thompson, NY Chen, H Karmouty-Q, U Fischer, M Kasper, D Klein, JW Ritchey, MR Blackburn, AM Westendorf, M Stuschke, V Jendrossek
    Cancer Res., 2016;76(10):3045-56.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Osteopontin depletion decreases inflammation and gastric epithelial proliferation during Helicobacter pylori infection in mice.
    Authors: Park J, Lee S, Go D, Kim H, Kwon H, Kim D
    Lab Invest, 2015;95(6):660-71.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. Identification of therapeutic targets for glioblastoma by network analysis.
    Authors: Friedmann-Morvinski D, Bhargava V, Gupta S, Verma I, Subramaniam S
    Oncogene, 2015;35(5):608-20.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  14. Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.
    Authors: Mu X, Espanol-Suner R, Mederacke I, Affo S, Manco R, Sempoux C, Lemaigre F, Adili A, Yuan D, Weber A, Unger K, Heikenwalder M, Leclercq I, Schwabe R
    J Clin Invest, 2015;125(10):3891-903.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. Loss of JUNB/AP-1 promotes invasive prostate cancer.
    Authors: Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E
    Cell Death Differ, 2015;22(4):574-82.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Cutting edge: Role of osteopontin and integrin alphav in T cell-mediated anti-inflammatory responses in endotoxemia.
    Authors: Inoue M, Shinohara M
    J Immunol, 2015;194(12):5595-8.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  17. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2015;18(1):145-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  18. Colony-stimulating factor-1 signaling suppresses renal crystal formation.
    Authors: Taguchi K, Okada A, Kitamura H, Yasui T, Naiki T, Hamamoto S, Ando R, Mizuno K, Kawai N, Tozawa K, Asano K, Tanaka M, Miyoshi I, Kohri K
    J Am Soc Nephrol, 2014;25(8):1680-97.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  19. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  20. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
    Authors: Kusumbe A, Ramasamy S, Adams R
    Nature, 2014;507(7492):323-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  21. Hydrogen peroxide regulates osteopontin expression through activation of transcriptional and translational pathways.
    Authors: Lyle A, Remus E, Fan A, Lassegue B, Walter G, Kiyosue A, Griendling K, Taylor W
    J Biol Chem, 2014;289(1):275-85.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  22. Osteopontin expression by CD103- dendritic cells drives intestinal inflammation.
    Authors: Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes D, Panoutsakopoulou V
    Proc Natl Acad Sci U S A, 2014;111(9):E856-65.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  23. An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells.
    Authors: Chen Q, Shou P, Zhang L, Xu C, Zheng C, Han Y, Li W, Huang Y, Zhang X, Shao C, Roberts A, Rabson A, Ren G, Zhang Y, Wang Y, Denhardt D, Shi Y
    Stem Cells, 2014;32(2):327-37.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  24. PGC-1alpha induces SPP1 to activate macrophages and orchestrate functional angiogenesis in skeletal muscle.
    Authors: Rowe G, Raghuram S, Jang C, Nagy J, Patten I, Goyal A, Chan M, Liu L, Jiang A, Spokes K, Beeler D, Dvorak H, Aird W, Arany Z
    Circ Res, 2014;115(5):504-17.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  25. Impaired hepatocyte maturation, abnormal expression of biliary transcription factors and liver fibrosis in C/EBPalpha(Cebpa)-knockout mice.
    Authors: Akai Y, Oitate T, Koike T, Shiojiri N
    Histol Histopathol, 2014;29(1):107-25.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  26. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-beta signaling pathways.
    Authors: Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N
    J Cell Sci, 2013;126(0):1392-405.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  27. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments.
    Authors: Park S, Wolfram P, Canty K, Harley B, Nombela-Arrieta C, Pivarnik G, Manis J, Beggs H, Silberstein L
    J Immunol, 2013;190(3):1094-102.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  28. Identification of a clonally expanding haematopoietic compartment in bone marrow.
    Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R
    EMBO J, 2013;32(2):219-30.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fluorescence
  29. Repression of osteocyte Wnt/β-catenin signaling is an early event in the progression of renal osteodystrophy.
    Authors: Sabbagh Y, Graciolli FG, O'Brien S, Tang W, Dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC
    J. Bone Miner. Res., 2012;27(8):1757-72.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  30. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012;82(12):1261-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  31. Cellular basis of tissue regeneration by omentum.
    Authors: Shah S, Lowery E, Braun RK
    PLoS ONE, 2012;7(6):e38368.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  32. The critical role of agrin in the hematopoietic stem cell niche.
    Authors: Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A
    Blood, 2011;118(10):2733-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  33. Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
    Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
    FASEB J., 2010;24(1):70-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  34. Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance.
    Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM
    Diabetes, 2010;59(4):935-46.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  35. Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse.
    Authors: Takanashi M, Oikawa K, Sudo K, Tanaka M, Fujita K, Ishikawa A, Nakae S, Kaspar RL, Matsuzaki M, Kudo M, Kuroda M
    Gene Ther., 2009;16(8):982-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  36. Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin.
    Authors: El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM
    Kidney Int., 2009;75(12):1297-307.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  37. Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis.
    Authors: Murugaiyan G, Mittal A, Weiner HL
    J. Immunol., 2008;181(11):7480-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  38. Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells.
    Authors: Terashima Y, Shimabukuro Y, Terashima H, Ozasa M, Terakura M, Ikezawa K, Hashikawa T, Takedachi M, Oohara H, Yamada S, Murakami S
    J. Cell. Physiol., 2008;216(3):640-50.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  39. In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression.
    Authors: Marcondes MC, Poling M, Watry DD, Hall D, Fox HS
    Cell. Immunol., 2008;254(1):56-62.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: WB
  40. Polyomavirus middle T antigen induces the transcription of osteopontin, a gene important for the migration of transformed cells.
    Authors: Whalen KA, Weber GF, Benjamin TL, Schaffhausen BS
    J. Virol., 2008;82(10):4946-54.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  41. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets.
    Authors: Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V
    Nat. Med., 2007;13(5):570-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  42. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
    Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
    Eur. Respir. J., 2006;28(2):275-85.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  43. Aggregation of embryonic stem cells induces Nanog repression and primitive endoderm differentiation.
    Authors: Hamazaki T, Oka M, Yamanaka S, Terada N
    J. Cell. Sci., 2004;117(0):5681-6.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  44. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.
    Authors: Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C
    Arthritis Rheum., 2004;50(8):2685-94.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
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