Mouse Osteopontin/OPN Antibody

Catalog # Availability Size / Price Qty
AF808
AF808-SP
Osteopontin/OPN  in C2C12 Mouse Cell Line and Mouse Splenocytes.
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Product Details
Citations (66)
FAQs
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Reviews (2)

Mouse Osteopontin/OPN Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Osteopontin (OPN) in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human OPN is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R&D Systems, Catalog # 441-OP)
Leu17-Asn294 (Glu99Gly)
Accession # Q547B5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.30 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Mouse Osteopontin/OPN Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Osteopontin/OPN Biotinylated Antibody (Catalog # BAF808)

Standard: Recombinant Mouse Osteopontin (OPN) Protein (Catalog # 441-OP)

Neutralization
Measured by its ability to neutralize Osteopontin/OPN-mediated adhesion of the HEK293 human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 2 µg/mL Recombinant Mouse Osteopontin/OPN.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Osteopontin/OPN  in C2C12 Mouse Cell Line and Mouse Splenocytes. View Larger

Osteopontin/OPN in C2C12 Mouse Cell Line and Mouse Splenocytes. Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Osteopontin/OPN in Mouse Thymus. View Larger

Osteopontin/OPN in Mouse Thymus. Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Mouse Osteopontin/OPN Antibody. View Larger

Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Mouse Osteopontin/OPN Antibody. Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Osteopontin/OPN (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The ND50 is typically 1‑3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteopontin/OPN

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

References
  1. Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21.
  2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

Product Datasheets

Citations for Mouse Osteopontin/OPN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

66 Citations: Showing 1 - 10
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  1. Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells
    Authors: DK Wasgewatte, EJ Mackie, CN Pagel
    Skelet Muscle, 2019;9(1):6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Pulmonary arterial remodelling by deficiency of peroxisome proliferator-activated receptor-? in murine vascular smooth muscle cells occurs independently of obesity-related pulmonary hypertension
    Authors: E Caglayan, M Trappiel, A Behringer, EM Berghausen, M Odenthal, E Wellnhofer, K Kappert
    Respir. Res., 2019;20(1):42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells
    Authors: M Zhao, F Tao, A Venkatrama, Z Li, SE Smith, J Unruh, S Chen, C Ward, P Qian, JM Perry, H Marshall, J Wang, XC He, L Li
    Cell Rep, 2019;26(3):652-669.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Osteopontin in the host response to Leishmania amazonensis
    Authors: E Giraud, E Rouault, L Fiette, JH Colle, D Smirlis, E Melanitou
    BMC Microbiol., 2019;19(1):32.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients
    Authors: H Hu, Z Liu, C Liu
    Oncol Lett, 2019;17(3):2788-2794.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
    Authors: KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Metabolic modulation of acetaminophen-induced hepatotoxicity by osteopontin
    Authors: Y Wen, C Wang, J Gu, C Yu, K Wang, X Sun, Y Sun, H Wu, Y Tong, Q Xia, X Kong
    Cell. Mol. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
    Authors: Q Zeng, X Luo, M Han, W Liu, H Li
    EBioMedicine, 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  9. H3K36 trimethylation mediated by SETD2 regulates the fate of bone marrow mesenchymal stem cells
    Authors: L Wang, N Niu, L Li, R Shao, H Ouyang, W Zou
    PLoS Biol., 2018;16(11):e2006522.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
    Authors: J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes
    Nat. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Molecular fingerprinting of On-Off direction selective retinal ganglion cells across species and relevance to primate visual circuits
    Authors: OS Dhande, BK Stafford, K Franke, R El-Danaf, KA Percival, AH Phan, P Li, BJ Hansen, PL Nguyen, P Berens, WR Taylor, E Callaway, T Euler, AD Huberman
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Section
    Applications: IHC
  12. Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression
    Authors: MN Lee, HS Hwang, SH Oh, A Roshanzade, JW Kim, JH Song, ES Kim, JT Koh
    Exp. Mol. Med., 2018;50(11):142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
    Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
    PLoS ONE, 2018;13(10):e0206756.
    Species: Mouse
    Sample Types: Cell Lysate
    Applications: Western Blot
  14. Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration
    Authors: HA Himburg, CM Termini, L Schlussel, J Kan, M Li, L Zhao, T Fang, JP Sasine, VY Chang, JP Chute
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Authors: KH Kim, SH Kim, DH Han, YS Jo, YH Lee, MS Lee
    Sci Rep, 2018;8(1):6789.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation
    Authors: AM D'Souza, Y Jiang, A Cast, L Valanejad, M Wright, K Lewis, M Kumbaji, S Shah, D Smithrud, R Karns, S Shin, N Timchenko
    Cell Mol Gastroenterol Hepatol, 2018;6(3):239-255.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis
    Authors: C Hernandez, P Huebener, JP Pradere, DJ Antoine, RA Friedman, RF Schwabe
    J. Clin. Invest., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits
    Authors: W Ding, K Yousefi, S Goncalves, BJ Goldstein, AL Sabater, A Kloosterbo, P Ritter, G Lambert, AJ Mendez, LA Shehadeh
    JCI Insight, 2018;3(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. Dentin Sialoprotein is a Novel Substrate of Matrix Metalloproteinase 9 in vitro and in vivo
    Authors: G Yuan, L Chen, J Feng, G Yang, Q Ni, X Xu, C Wan, M Lindsey, KJ Donly, M MacDougall, Z Chen, S Chen
    Sci Rep, 2017;7(0):42449.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells
    Authors: N Guidi, M Sacma, L Ständker, K Soller, G Marka, K Eiwen, JM Weiss, F Kirchhoff, T Weil, JA Cancelas, MC Florian, H Geiger
    EMBO J, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  22. Four alpha ganglion cell types in mouse retina: Function, structure, and molecular signatures
    Authors: B Krieger, M Qiao, DL Rousso, JR Sanes, M Meister
    PLoS ONE, 2017;12(7):e0180091.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  23. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017;19(3):189-201.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway
    Authors: CH Chen, YM Sue, CY Cheng, YC Chen, CT Liu, YH Hsu, PA Hwang, NJ Huang, TH Chen
    Sci Rep, 2017;7(0):40183.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin, TGF-?1, IL-13 and oxidative stress
    Authors: VCA de Souza, TA Pereira, VW Teixeira, H Carvalho, MCAB de Castro, CG D'assunção, AF de Barros, CL Carvalho, VMB de Lorena, VMA Costa, ÁAC Teixeira, RCBQ Figueiredo, SA de Oliveir
    World J. Gastroenterol., 2017;23(28):5146-5157.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC paraffin embedded
  26. Sox11 Expression Promotes Regeneration of Some Retinal Ganglion Cell Types but Kills Others
    Authors: MW Norsworthy, F Bei, R Kawaguchi, Q Wang, NM Tran, Y Li, B Brommer, Y Zhang, C Wang, JR Sanes, G Coppola, Z He
    Neuron, 2017;94(6):1112-1120.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Monocyte Chemotactic Protein-1-Interleukin-6-Osteopontin Pathway of Intra-Aneurysmal Tissue Healing
    Authors: K Hosaka, K Rojas, HZ Fazal, MB Schneider, J Shores, V Federico, M McCord, L Lin, B Hoh
    Stroke, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  28. Alternatively activated macrophages determine repair of the infarcted adult murine heart
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response
    Authors: K Zhao, M Zhang, L Zhang, P Wang, G Song, B Liu, H Wu, Z Yin, C Gao
    Sci Rep, 2016;6(0):23771.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Neutralization
  30. Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis
    Authors: F Wirsdörfer, S de Leve, F Cappuccini, T Eldh, AV Meyer, E Gau, LF Thompson, NY Chen, H Karmouty-Q, U Fischer, M Kasper, D Klein, JW Ritchey, MR Blackburn, AM Westendorf, M Stuschke, V Jendrossek
    Cancer Res., 2016;76(10):3045-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Disease-modifying effects of orally bioavailable NF-?B inhibitors in dystrophin-deficient muscle
    JCI Insight, 2016;1(21):e90341.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9
    Authors: Yuta Morisaki
    Sci Rep, 2016;6(0):27354.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - PFA fixed
  33. The P2X4 receptor is required for neuroprotection via ischemic preconditioning
    Sci Rep, 2016;6(0):25893.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  34. Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.
    Authors: Mu X, Espanol-Suner R, Mederacke I, Affo S, Manco R, Sempoux C, Lemaigre F, Adili A, Yuan D, Weber A, Unger K, Heikenwalder M, Leclercq I, Schwabe R
    J Clin Invest, 2015;125(10):3891-903.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  35. Loss of JUNB/AP-1 promotes invasive prostate cancer.
    Authors: Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E
    Cell Death Differ, 2015;22(4):574-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  36. Identification of therapeutic targets for glioblastoma by network analysis.
    Authors: Friedmann-Morvinski D, Bhargava V, Gupta S, Verma I, Subramaniam S
    Oncogene, 2015;35(5):608-20.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Osteopontin depletion decreases inflammation and gastric epithelial proliferation during Helicobacter pylori infection in mice.
    Authors: Park J, Lee S, Go D, Kim H, Kwon H, Kim D
    Lab Invest, 2015;95(6):660-71.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2015;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. Cutting edge: Role of osteopontin and integrin alphav in T cell-mediated anti-inflammatory responses in endotoxemia.
    Authors: Inoue M, Shinohara M
    J Immunol, 2015;194(12):5595-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  40. An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells.
    Authors: Chen Q, Shou P, Zhang L, Xu C, Zheng C, Han Y, Li W, Huang Y, Zhang X, Shao C, Roberts A, Rabson A, Ren G, Zhang Y, Wang Y, Denhardt D, Shi Y
    Stem Cells, 2014;32(2):327-37.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Impaired hepatocyte maturation, abnormal expression of biliary transcription factors and liver fibrosis in C/EBPalpha(Cebpa)-knockout mice.
    Authors: Akai Y, Oitate T, Koike T, Shiojiri N
    Histol Histopathol, 2014;29(1):107-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  42. Osteopontin expression by CD103- dendritic cells drives intestinal inflammation.
    Authors: Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes D, Panoutsakopoulou V
    Proc Natl Acad Sci U S A, 2014;111(9):E856-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  43. PGC-1alpha induces SPP1 to activate macrophages and orchestrate functional angiogenesis in skeletal muscle.
    Authors: Rowe G, Raghuram S, Jang C, Nagy J, Patten I, Goyal A, Chan M, Liu L, Jiang A, Spokes K, Beeler D, Dvorak H, Aird W, Arany Z
    Circ Res, 2014;115(5):504-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  44. Hydrogen peroxide regulates osteopontin expression through activation of transcriptional and translational pathways.
    Authors: Lyle A, Remus E, Fan A, Lassegue B, Walter G, Kiyosue A, Griendling K, Taylor W
    J Biol Chem, 2014;289(1):275-85.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  45. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
    Authors: Kusumbe A, Ramasamy S, Adams R
    Nature, 2014;507(7492):323-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  46. Colony-stimulating factor-1 signaling suppresses renal crystal formation.
    Authors: Taguchi K, Okada A, Kitamura H, Yasui T, Naiki T, Hamamoto S, Ando R, Mizuno K, Kawai N, Tozawa K, Asano K, Tanaka M, Miyoshi I, Kohri K
    J Am Soc Nephrol, 2014;25(8):1680-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  47. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  48. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-beta signaling pathways.
    Authors: Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N
    J Cell Sci, 2013;126(0):1392-405.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  49. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments.
    Authors: Park S, Wolfram P, Canty K, Harley B, Nombela-Arrieta C, Pivarnik G, Manis J, Beggs H, Silberstein L
    J Immunol, 2013;190(3):1094-102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-OCT embedded
  50. Identification of a clonally expanding haematopoietic compartment in bone marrow.
    Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R
    EMBO J, 2013;32(2):219-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fluorescence
  51. Cellular basis of tissue regeneration by omentum.
    Authors: Shah S, Lowery E, Braun RK
    PLoS ONE, 2012;7(6):e38368.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  52. Repression of osteocyte Wnt/β-catenin signaling is an early event in the progression of renal osteodystrophy.
    Authors: Sabbagh Y, Graciolli FG, O'Brien S, Tang W, Dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC
    J. Bone Miner. Res., 2012;27(8):1757-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  53. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012;82(12):1261-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  54. The critical role of agrin in the hematopoietic stem cell niche.
    Authors: Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A
    Blood, 2011;118(10):2733-42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  55. Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance.
    Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM
    Diabetes, 2010;59(4):935-46.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  56. Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
    Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
    FASEB J., 2010;24(1):70-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  57. Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse.
    Authors: Takanashi M, Oikawa K, Sudo K, Tanaka M, Fujita K, Ishikawa A, Nakae S, Kaspar RL, Matsuzaki M, Kudo M, Kuroda M
    Gene Ther., 2009;16(8):982-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  58. Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin.
    Authors: El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM
    Kidney Int., 2009;75(12):1297-307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  59. Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells.
    Authors: Terashima Y, Shimabukuro Y, Terashima H, Ozasa M, Terakura M, Ikezawa K, Hashikawa T, Takedachi M, Oohara H, Yamada S, Murakami S
    J. Cell. Physiol., 2008;216(3):640-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  60. Polyomavirus middle T antigen induces the transcription of osteopontin, a gene important for the migration of transformed cells.
    Authors: Whalen KA, Weber GF, Benjamin TL, Schaffhausen BS
    J. Virol., 2008;82(10):4946-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  61. Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis.
    Authors: Murugaiyan G, Mittal A, Weiner HL
    J. Immunol., 2008;181(11):7480-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  62. In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression.
    Authors: Marcondes MC, Poling M, Watry DD, Hall D, Fox HS
    Cell. Immunol., 2008;254(1):56-62.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  63. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets.
    Authors: Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V
    Nat. Med., 2007;13(5):570-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  64. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
    Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
    Eur. Respir. J., 2006;28(2):275-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  65. Aggregation of embryonic stem cells induces Nanog repression and primitive endoderm differentiation.
    Authors: Hamazaki T, Oka M, Yamanaka S, Terada N
    J. Cell. Sci., 2004;117(0):5681-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  66. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.
    Authors: Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C
    Arthritis Rheum., 2004;50(8):2685-94.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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Mouse Osteopontin/OPN Antibody
By Anonymous on 08/31/2018
Application: ICC/IF Sample Tested: Bone marrow Species: Mouse

Mouse Osteopontin/OPN Antibody
By Anonymous on 04/26/2017
Application: ICC/IF Sample Tested: Cell Culture Samples Species: Rat