Mouse Osteopontin/OPN Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF808
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Mouse Osteopontin/OPN Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Osteopontin/OPN in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant human Osteopontin/OPN is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R&D Systems, Catalog # 441-OP)
Leu17-Asn294 (Glu99Gly)
Accession # Q547B5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP)

Mouse Osteopontin/OPN Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Osteopontin/OPN Antibody (Catalog # AF808)

Standard: Recombinant Mouse Osteopontin/OPN Protein (Catalog # 441-OP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteopontin/OPN

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T-cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40% - 80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

References
  1. Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21.
  2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

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Citations for Mouse Osteopontin/OPN Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Secreted osteopontin from CD4+ T�cells limits acute graft-versus-host disease
    Authors: N Aggarwal, ME Deerhake, D DiPalma, SK Shahi, MR Gaggioli, AK Mangalam, ML Shinohara
    Cell Reports, 2021;37(13):110170.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  2. Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells
    Authors: N Guidi, M Sacma, L Ständker, K Soller, G Marka, K Eiwen, JM Weiss, F Kirchhoff, T Weil, JA Cancelas, MC Florian, H Geiger
    EMBO J, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  3. Mesenchymal stem cells from cortical bone demonstrate increased clonal incidence, potency, and developmental capacity compared to their bone marrow-derived counterparts
    J Tissue Eng, 2016;7(0):2041731416661.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Cutting edge: Role of osteopontin and integrin alphav in T cell-mediated anti-inflammatory responses in endotoxemia.
    Authors: Inoue M, Shinohara M
    J Immunol, 2015;194(12):5595-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  5. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.
    Authors: Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C
    Arthritis Rheum., 2004;50(8):2685-94.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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Reviews for Mouse Osteopontin/OPN Biotinylated Antibody

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Mouse Osteopontin/OPN Biotinylated Antibody
By Anonymous on 12/17/2018
Application: ICC/IF Sample Tested: osteoblast cells derived from mouse limb mesenchymal cells,osteoblasts derived from mouse limb mesenchymal cells Species: Mouse

Primary antibody used at 1:500 and was incubated overnight at 4°C.