Mouse PD-L2/B7-DC Antibody MAB1022: R&D Systems

Mouse PD-L2/B7-DC Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) PD-L2 is observed and no cross-reactivity with rhB7-1, recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H2, rmB7‑H3, or rmB7-H4 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 168633
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse PD-L2/B7-DC
    Met1-Arg219
    Accession # Q9WUL5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse PD-L2/B7-DC Fc Chimera (Catalog # 1022-PL)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PD‑L2/B7‑DC in HEK293 Human Cell Line Transfected with Mouse PD-L2/B7-DC by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either mouse PD-L2/B7-DC (filled histogram) or irrelevant transfectants (open histogram) was stained with Rat Anti-Mouse PD‑L2/B7‑DC Monoclonal Antibody (Catalog # MAB1022), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-L2/B7-DC

Mouse Programmed Death Ligand 2 (PD-L2), also named B7DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and B7-H3. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse PD-L2 cDNA encodes a 247 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 199 aa extracellular region containing one V-like and one C-like Ig domain, a 23 aa transmembrane region, and only a 5 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 72% aa sequence identity. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. Mouse PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. PD-L2 is constitutively expressed in lymphoid and non-lymphoid organs (1-4). The expression of PD-L2 is detected on dendritic cells, thymic epithelial cells and IFN-gamma treated monocytes. PD-L2 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co-stimulatory function for the PD-1 ligands on resting T cells has also been reported.

  • References:
    1. Latchman, Y. et al. (2001) Nature Immun. 2:261.
    2. Tseng, B.S-Y. et al. (2001) J. Exp. Med. 193:839.
    3. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
    4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  • Long Name:
    Programmed Death-1 Ligand-2
  • Entrez Gene IDs:
    80380 (Human); 58205 (Mouse); 102146227 (Cynomolgus Monkey)
  • Alternate Names:
    B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PD-L2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2
Related Research Areas
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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