Mouse Periostin/OSF-2 Isoform 2 Antibody

Name: Mouse Periostin/OSF-2 Isoform 2 Antibody
Brand: R&D Systems
SKU: AF2955-SP
Rating: 4.7 (6 reviews)

AF2955 13 Citations 6 Reviews Datasheet / COA / SDS

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AF2955
AF2955-SP

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Periostin/OSF‑2 in Rat Mesenchymal Stem Cells.

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Citations (13)

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Reviews (6)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Periostin/OSF-2 Isoform 2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Periostin/OSF‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant rat Periostin/OSF-2 and recombinant human Periostin/OSF-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2
Asn24-Gln811
Accession # Q62009

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize Periostin/OSF‑2-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Periostin/OSF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry

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Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. Periostin/OSF-2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Neutralization

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Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral-ization by Mouse Periostin/ OSF‑2 Antibody. Recombinant Mouse Periostin/OSF-2 (Catalog # 2955-F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recom-binant Mouse Periostin/OSF-2 (5 µg/mL) is neutralized (green line) by increasing concent-rations of Goat Anti-Mouse Periostin/ OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND₅₀ is typically 1-5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha _v beta ₃ and alpha _v beta ₅ integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.

References

Clout, N.J. and D. Tisi (2003) Structure 11:197.
Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
Gillan, L. et al. (2002) Cancer Res. 62:5358.
Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
Takeshita, S. et al. (1993) Biochem. J. 294:271.
Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
Swiss-Prot Accession # Q62009.

Long Name

Osteoblast Specific Factor 2

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Alternate Names

Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse Periostin/OSF-2 Isoform 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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FGFR3 in Periosteal Cells Drives Cartilage-to-Bone Transformation in Bone Repair
Authors: A Julien, S Perrin, O Duchamp de, C Carvalho, M Bensidhoum, L Legeai-Mal, C Colnot
Stem Cell Reports, 2020;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to ?-adrenergic stimulation-induced cardiac fibrosis
Authors: S Takahara, SI Inoue, S Miyagawa-T, K Matsuura, Y Nakashima, T Niihori, Y Matsubara, Y Saiki, Y Aoki
EBioMedicine, 2019;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
Authors: KM Pineault, JY Song, KM Kozloff, D Lucas, DM Wellik
Nat Commun, 2019;10(1):3168.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2018;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer
Authors: V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid
Cancer Res., 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Periostin deletion suppresses late-phase response in mouse experimental allergic conjunctivitis
Authors: Y Asada, M Okano, W Ishida, S Iwamoto, K Fukuda, T Hirakata, N Tada, A Fukushima, N Ebihara, A Kudo, A Matsuda
Allergol Int, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
Skelet Muscle, 2017;7(1):3.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis
Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid
Nat Cell Biol, 2016;0(0):.
A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
Authors: Pan T, Zhang R, Arita M, Bogdanovich S, Adams S, Gara S, Wagener R, Khurana T, Birk D, Chu M
J Biol Chem, 2014;289(15):10293-307.
Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu
PLoS ONE, 2013;8(11):e79753.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
Lab Invest, 2012;92(11):1518-26.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
Authors: Xu BJ, Yan W, Jovanovic B
Proteomics, 2010;10(13):2458-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
Authors: Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J
J. Biol. Chem., 2008;283(26):17991-8001.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot