Mouse Periostin/OSF-2 Isoform 2 Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Periostin/OSF‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat Periostin/OSF-2 and less than 20% cross-reactivity with recombinant human Periostin/OSF-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2
    Asn24-Gln811
    Accession # Q62009
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Periostin/OSF‑2-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Periostin/OSF‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral­ization by Mouse Periostin/
OSF‑2 Antibody.
Recombinant Mouse Periostin/OSF‑2 (Cata­log # 2955‑F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recom­binant Mouse Periostin/OSF‑2 (5 µg/mL) is neutralized (green line) by increasing concent­rations of Goat Anti-Mouse Periostin/
OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND50 is typically 1-5 µg/mL.
Immunocytochemistry
Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. Periostin/OSF‑2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Periostin/OSF-2

Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.

  • References:
    1. Clout, N.J. and D. Tisi (2003) Structure 11:197.
    2. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
    3. Gillan, L. et al. (2002) Cancer Res. 62:5358.
    4. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
    5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
    6. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
    7. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
    8. Takeshita, S. et al. (1993) Biochem. J. 294:271.
    9. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
    10. Swiss-Prot Accession # Q62009.
  • Long Name:
    Osteoblast Specific Factor 2
  • Entrez Gene IDs:
    10631 (Human); 50706 (Mouse); 361945 (Rat)
  • Alternate Names:
    Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
    Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
    Skelet Muscle, 2017;7(1):3.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis
    Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid
    Nat Cell Biol, 2016;0(0):.
  3. A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
    Authors: Pan T, Zhang R, Arita M, Bogdanovich S, Adams S, Gara S, Wagener R, Khurana T, Birk D, Chu M
    J Biol Chem, 2014;289(15):10293-307.
  4. Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
    Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu
    PLoS ONE, 2013;8(11):e79753.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  5. Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
    Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
    Lab Invest, 2012;92(11):1518-26.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
    Authors: Xu BJ, Yan W, Jovanovic B
    Proteomics, 2010;10(13):2458-70.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
    Authors: Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J
    J. Biol. Chem., 2008;283(26):17991-8001.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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