Mouse Periostin/OSF-2 Isoform 2 Antibody

Catalog # Availability Size / Price Qty
AF2955
AF2955-SP
Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral­ization by Mouse Periostin/OSF‑2 Antibody.
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Product Details
Citations (9)
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Mouse Periostin/OSF-2 Isoform 2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Periostin/OSF‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant rat Periostin/OSF-2 and recombinant human Periostin/OSF-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2
Asn24-Gln811
Accession # Q62009
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Periostin/OSF‑2-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Periostin/OSF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral­ization by Mouse Periostin/OSF‑2 Antibody. View Larger

Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral­ization by Mouse Periostin/
OSF‑2 Antibody.
Recombinant Mouse Periostin/OSF‑2 (Cata­log # 2955‑F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recom­binant Mouse Periostin/OSF‑2 (5 µg/mL) is neutralized (green line) by increasing concent­rations of Goat Anti-Mouse Periostin/
OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND50 is typically 1-5 µg/mL.

Immunocytochemistry Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. View Larger

Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. Periostin/OSF‑2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.

References
  1. Clout, N.J. and D. Tisi (2003) Structure 11:197.
  2. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  3. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  4. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  7. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
  8. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  9. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
  10. Swiss-Prot Accession # Q62009.
Long Name
Osteoblast Specific Factor 2
Entrez Gene IDs
10631 (Human); 50706 (Mouse); 361945 (Rat)
Alternate Names
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

Product Datasheets

Citations for Mouse Periostin/OSF-2 Isoform 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer
    Authors: V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid
    Cancer Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  3. Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
    Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
    Skelet Muscle, 2017;7(1):3.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  4. Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis
    Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid
    Nat Cell Biol, 2016;0(0):.
  5. A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
    Authors: Pan T, Zhang R, Arita M, Bogdanovich S, Adams S, Gara S, Wagener R, Khurana T, Birk D, Chu M
    J Biol Chem, 2014;289(15):10293-307.
  6. Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
    Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu
    PLoS ONE, 2013;8(11):e79753.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neut
  7. Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
    Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
    Lab Invest, 2012;92(11):1518-26.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  8. Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
    Authors: Xu BJ, Yan W, Jovanovic B
    Proteomics, 2010;10(13):2458-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  9. Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
    Authors: Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J
    J. Biol. Chem., 2008;283(26):17991-8001.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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