Mouse Periostin/OSF-2 Isoform 2 Antibody Summary
Asn24-Gln811
Accession # Q62009
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Cell Adhesion Mediated by Periostin/OSF‑2 and Neutralization by Mouse Periostin/
OSF‑2 Antibody.
Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955‑F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Periostin/OSF‑2 (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Periostin/
OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND50 is typically 1-5 µg/mL.

Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. Periostin/OSF‑2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Periostin/OSF-2
Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.
- Clout, N.J. and D. Tisi (2003) Structure 11:197.
- Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
- Gillan, L. et al. (2002) Cancer Res. 62:5358.
- Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
- Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
- Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
- Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
- Takeshita, S. et al. (1993) Biochem. J. 294:271.
- Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
- Swiss-Prot Accession # Q62009.
Product Datasheets
Citations for Mouse Periostin/OSF-2 Isoform 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
Filter your results:
Filter by:
-
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2018;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer
Authors: V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid
Cancer Res., 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neut -
Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
Skelet Muscle, 2017;7(1):3.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB -
Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis
Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid
Nat Cell Biol, 2016;0(0):.
-
A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
Authors: Pan T, Zhang R, Arita M, Bogdanovich S, Adams S, Gara S, Wagener R, Khurana T, Birk D, Chu M
J Biol Chem, 2014;289(15):10293-307.
-
Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu
PLoS ONE, 2013;8(11):e79753.
Species: Rat
Sample Types: Whole Cells
Applications: Neut -
Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
Lab Invest, 2012;92(11):1518-26.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB -
Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
Authors: Xu BJ, Yan W, Jovanovic B
Proteomics, 2010;10(13):2458-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB -
Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
Authors: Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J
J. Biol. Chem., 2008;283(26):17991-8001.
Species: Human
Sample Types: Cell Lysates
Applications: WB
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsCell and Tissue Staining Kits
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Mouse Periostin/OSF-2 Isoform 2 Antibody
There are currently no reviews for this product. Be the first to review Mouse Periostin/OSF-2 Isoform 2 Antibody and earn rewards!
Have you used Mouse Periostin/OSF-2 Isoform 2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image