Mouse Periostin/OSF-2 Isoform 2 Antibody

Catalog # Availability Size / Price Qty
AF2955
AF2955-SP
Periostin/OSF‑2 in Rat Mesenchymal Stem Cells.
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (6)

Mouse Periostin/OSF-2 Isoform 2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Periostin/OSF‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant rat Periostin/OSF-2 and recombinant human Periostin/OSF-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2
Asn24-Gln811
Accession # Q62009
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Periostin/OSF‑2-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Periostin/OSF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Periostin/OSF-2 antibody in Rat Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. Periostin/OSF-2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Neutralization Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral-ization by Mouse Periostin/ OSF‑2 Antibody. View Larger

Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral-ization by Mouse Periostin/ OSF‑2 Antibody. Recombinant Mouse Periostin/OSF-2 (Catalog # 2955-F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recom-binant Mouse Periostin/OSF-2 (5 µg/mL) is neutralized (green line) by increasing concent-rations of Goat Anti-Mouse Periostin/ OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND50 is typically 1-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.

References
  1. Clout, N.J. and D. Tisi (2003) Structure 11:197.
  2. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  3. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  4. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  7. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
  8. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  9. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
  10. Swiss-Prot Accession # Q62009.
Long Name
Osteoblast Specific Factor 2
Entrez Gene IDs
10631 (Human); 50706 (Mouse); 361945 (Rat)
Alternate Names
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

Product Datasheets

Citations for Mouse Periostin/OSF-2 Isoform 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. FGFR3 in Periosteal Cells Drives Cartilage-to-Bone Transformation in Bone Repair
    Authors: A Julien, S Perrin, O Duchamp de, C Carvalho, M Bensidhoum, L Legeai-Mal, C Colnot
    Stem Cell Reports, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to ?-adrenergic stimulation-induced cardiac fibrosis
    Authors: S Takahara, SI Inoue, S Miyagawa-T, K Matsuura, Y Nakashima, T Niihori, Y Matsubara, Y Saiki, Y Aoki
    EBioMedicine, 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Authors: KM Pineault, JY Song, KM Kozloff, D Lucas, DM Wellik
    Nat Commun, 2019;10(1):3168.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer
    Authors: V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid
    Cancer Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Periostin deletion suppresses late-phase response in mouse experimental allergic conjunctivitis
    Authors: Y Asada, M Okano, W Ishida, S Iwamoto, K Fukuda, T Hirakata, N Tada, A Fukushima, N Ebihara, A Kudo, A Matsuda
    Allergol Int, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
    Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
    Skelet Muscle, 2017;7(1):3.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis
    Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid
    Nat Cell Biol, 2016;0(0):.
  9. A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
    Authors: Pan T, Zhang R, Arita M, Bogdanovich S, Adams S, Gara S, Wagener R, Khurana T, Birk D, Chu M
    J Biol Chem, 2014;289(15):10293-307.
  10. Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
    Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu
    PLoS ONE, 2013;8(11):e79753.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
    Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
    Lab Invest, 2012;92(11):1518-26.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
    Authors: Xu BJ, Yan W, Jovanovic B
    Proteomics, 2010;10(13):2458-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
    Authors: Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J
    J. Biol. Chem., 2008;283(26):17991-8001.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Mouse Periostin/OSF-2 Isoform 2 Antibody

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Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 07/19/2021
Application: WB Sample Tested: Heart tissue Species: Mouse

Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 06/29/2021
Application: IHC Sample Tested: Bladder tissue Species: Rat

Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 05/10/2020
Application: WB Sample Tested: Adult heart Species: Mouse

3 mice hearts extractions expressing periostin after MI. HSP60 used as loading marker.


Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 05/10/2020
Application: ICC/IF Sample Tested: primary cardiac fibroblasts Species: Rat

primary rat cardiac fiboblasts. periostin in green and DAPI in blue


Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 05/10/2020
Application: ICC/IF Sample Tested: Adult heart Species: Mouse

mouse heart post MI fixed in PFA stained to periostin in red, troponin I in green and DAPI in blue


Mouse Periostin/OSF-2 Isoform 2 Antibody
By Anonymous on 06/17/2019
Application: WB Sample Tested: Tissue Lysates Species: Mouse