Mouse Periostin/OSF-2 Isoform 2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2955
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Mouse Periostin/OSF-2 Isoform 2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Periostin/OSF‑2 Isoform 2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human OSF-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2 Isoform 2
Asn24-Gln811
Accession # Q62009
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)

Mouse Periostin/OSF-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Periostin/OSF‑2 Antibody (Catalog # MAB2955)

Standard: Recombinant Mouse Periostin/OSF-2 Protein, CF (Catalog # 2955-F2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.

References
  1. Clout, N.J. and D. Tisi (2003) Structure 11:197.
  2. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  3. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  4. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  7. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
  8. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  9. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
  10. Swiss-Prot Accession # Q62009.
Long Name
Osteoblast Specific Factor 2
Entrez Gene IDs
10631 (Human); 50706 (Mouse); 361945 (Rat)
Alternate Names
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

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