Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 377313
View all MMP-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP‑7
Leu18-Leu264
Accession # AAA99983

Specificity

Detects the pro form of mouse MMP-7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse MMP‑2, -3, -8, -9, -12, or -24 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Pro‑MMP‑7 Antibody

Detection of Mouse Pro-MMP-7 antibody by Western Blot.

Detection of Mouse Pro‑MMP‑7 by Western Blot.

Western blot shows lysates of NS0 mouse myeloma cell line transfected with with mouse MMP-7. PVDF Membrane was probed with 2 µg/mL of Mouse Pro-MMP-7 Monoclonal Antibody (Catalog # MAB29671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, active rmMMP-7 was derived from the proteolytic activation of Recombinant Mouse MMP-7 (Catalog # 2967-MP) (20 ng/lane) and probed with 1 µg/mL of Mouse MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967). Specific bands were detected for Pro-MMP-7 at approximately 35 kDa and active MMP-7 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Pro‑MMP‑7 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: NS0 mouse myeloma cell line transfected with with mouse MMP-7

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP‑7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin, and versican (1). MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

References

  1. Woessner, J.F. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 532.

Long Name

Matrix Metalloproteinase 7

Alternate Names

Matrilysin, MMP7

Entrez Gene IDs

4316 (Human); 17393 (Mouse)

Gene Symbol

MMP7

UniProt

AAA99983

Additional MMP-7 Products

Product Documents for Mouse Pro‑MMP‑7 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Pro‑MMP‑7 Antibody

For research use only

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