Mouse MMP-7 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human MMP‑7 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) MMP-2, rmMMP-3, rmMMP-8, and rmMMP-9 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MMP‑7
    Leu18-Leu264
    Accession # AAA99983
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    50 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

MMP‑7 in Mouse Intestine. MMP‑7 was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse MMP-7 by Western Blot.
Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMP‑7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Mouse MMP‑7 by Simple WesternTM. Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP‑7 at approximately 25 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican (1). MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

  • References:
    1. Woessner, J.F. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 532.
  • Long Name:
    Matrix Metalloproteinase 7
  • Entrez Gene IDs:
    4316 (Human); 17393 (Mouse)
  • Alternate Names:
    EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Canonical Wnt Signaling Ameliorates Aging of Intestinal Stem Cells
    Authors: K Nalaparedd, KJ Nattamai, RS Kumar, R Karns, KA Wikenheise, LL Sampson, MM Mahe, N Sundaram, MB Yacyshyn, B Yacyshyn, MA Helmrath, Y Zheng, H Geiger
    Cell Rep, 2017;18(11):2608-2621.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Interleukin-17 promotes prostate cancer via MMP7-induced epithelial-to-mesenchymal transition
    Oncogene, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Interleukin-17 promotes formation and growth of prostate adenocarcinoma in mouse models.
    Authors: Zhang Q, Liu S, Ge D, Zhang Q, Xue Y, Xiong Z, Abdel-Mageed A, Myers L, Hill S, Rowan B, Sartor O, Melamed J, Chen Z, You Z
    Cancer Res, 2012;72(10):2589-99.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Matrix metalloproteinases contribute distinct roles in neuroendocrine prostate carcinogenesis, metastasis, and angiogenesis progression.
    Authors: Littlepage LE, Sternlicht MD, Rougier N
    Cancer Res., 2010;70(6):2224-34.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Matrix metalloproteinase 7 mediates mammary epithelial cell tumorigenesis through the ErbB4 receptor.
    Authors: Lynch CC, Vargo-Gogola T, Martin MD, Fingleton B, Crawford HC, Matrisian LM
    Cancer Res., 2007;67(14):6760-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Cell and Tissue Staining Kits
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Chicken Anti-Goat IgG Biotinylated Antibody

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