Mouse MMP-7 Antibody

Catalog # Availability Size / Price Qty
AF2967
AF2967-SP
MMP‑7 in Mouse Intestine.
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Product Details
Citations (6)
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Mouse MMP-7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human MMP‑7 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) MMP-2, rmMMP-3, rmMMP-8, and rmMMP-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP‑7
Leu18-Leu264
Accession # AAA99983
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry MMP‑7 in Mouse Intestine. View Larger

MMP‑7 in Mouse Intestine. MMP‑7 was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Mouse MMP-7 by Western Blot. View Larger

Detection of Mouse MMP-7 by Western Blot.
Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMP‑7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Mouse MMP‑7 by Simple WesternTM. View Larger

Detection of Mouse MMP‑7 by Simple WesternTM. Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP‑7 at approximately 25 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-7

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican (1). MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

References
  1. Woessner, J.F. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 532.
Long Name
Matrix Metalloproteinase 7
Entrez Gene IDs
4316 (Human); 17393 (Mouse)
Alternate Names
EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase

Product Datasheets

Citations for Mouse MMP-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche
    Authors: YM Nusse, AK Savage, P Marangoni, AKM Rosendahl-, TA Landman, FJ de Sauvage, RM Locksley, OD Klein
    Nature, 2018;559(7712):109-113.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Canonical Wnt Signaling Ameliorates Aging of Intestinal Stem Cells
    Authors: K Nalaparedd, KJ Nattamai, RS Kumar, R Karns, KA Wikenheise, LL Sampson, MM Mahe, N Sundaram, MB Yacyshyn, B Yacyshyn, MA Helmrath, Y Zheng, H Geiger
    Cell Rep, 2017;18(11):2608-2621.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Interleukin-17 promotes prostate cancer via MMP7-induced epithelial-to-mesenchymal transition
    Oncogene, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  4. Interleukin-17 promotes formation and growth of prostate adenocarcinoma in mouse models.
    Authors: Zhang Q, Liu S, Ge D, Zhang Q, Xue Y, Xiong Z, Abdel-Mageed A, Myers L, Hill S, Rowan B, Sartor O, Melamed J, Chen Z, You Z
    Cancer Res, 2012;72(10):2589-99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Matrix metalloproteinases contribute distinct roles in neuroendocrine prostate carcinogenesis, metastasis, and angiogenesis progression.
    Authors: Littlepage LE, Sternlicht MD, Rougier N
    Cancer Res., 2010;70(6):2224-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  6. Matrix metalloproteinase 7 mediates mammary epithelial cell tumorigenesis through the ErbB4 receptor.
    Authors: Lynch CC, Vargo-Gogola T, Martin MD, Fingleton B, Crawford HC, Matrisian LM
    Cancer Res., 2007;67(14):6760-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut

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