Mouse Progranulin/PGRN Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Progranulin/PGRN in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human Progranulin is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 333731
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Progranulin/PGRN
    Thr18-Leu589
    Accession # P28798
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse Progranulin (Catalog # 2557-PG) under non-reducing conditions only. We recommend (Catalog # AF2557) for Western blot applications.
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Progranulin in Mouse Kidney. Progranulin was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Progranulin Monoclonal Antibody (Catalog # MAB25571) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Progranulin/PGRN

Progranulin, also known as acrogranin, PC cell-derived growth factor (PCDGF) and epithelin/granulin precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it belongs to none of the well-established growth factor families (4). Mouse Progranulin is 589 amino acids (aa) in length and contains a 17 aa signal sequence and a 572 aa mature region that has four potential sites for N-linked glycosylation. It has a highly repetitive organization, containing seven tandem copies of a 55‑57 aa consensus motif that contains 12 conserved cysteine residues: VXCX5-6CX5CCX8CCX6CCXDX2HCCPX4CX5-6CX2 (1). Progranulin is secreted in an intact form (2, 4) or undergoes proteolysis leading to the release of multiple peptides made from the seven tandem repeats, the granulins (5‑7). Mouse Progranulin shares 87% and 75% aa sequence identity with rat and human Progranulin, respectively. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), development synovium of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation plus of wound response and tissue repair (4, 12‑13). High levels of Progranulin expression have been found to be associated with several human cancers, and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma (4, 5, 12, 14‑20).

  • References:
    1. Plowman, G.D. et al. (1992) J. Biol. Chem. 267:13073. 
    2. Zhou, J. et al. (1993) J. Biol. Chem. 268:10863. 
    3. Liu, Y. et al. (2007) BMC Cancer 7:22. 
    4. Xu, K. et al. (2007) J. Biol. Chem. 282:11347. 
    5. Davidson, B. et al. (2004) Cancer 100:2139. 
    6. Zanocco-Marani, T. et al. (1999) Cancer Res. 59:5331. 
    7. Lu, R. and G. Serrero (2000) Proc. Natl. Acad. Sci. U.S.A. 97:3993. 
    8. Sun, X. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:510. 
    9. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:727 
    10. Justen, H.P. et al. (2000) Mol. Cell Biol. Res. Commun. 3:165.
    11. Suzuki, M. and M. Nishiahara (2002) Mol. Genet. Metab. 75:31.
    12. He, Z. et al. (2003) Nat. Med. 9:225.
    13. Zhu, J. et al. (2002) Cell 111:867.
    14. He, Z. and A. Bateman (2003) J. Mol. Med. 81:600.
    15. Bateman, A. et al. (1990) Biochem. Biophys. Res. Commun. 173:1161.
    16. Gonzalez, E.M. et al. (2003) J. Biol. Chem. 278:38113.
    17. Jones, M.B. et al. (2003) Gynecol. Oncol. 88:S136.
    18. Wang, W. et al. (2003) Clin. Cancer Res. 9:2221.
    19. Zhang, H. and G. Serrero (1998) Proc. Natl. Acad. Sci. U.S.A. 95:14202.
  • Entrez Gene IDs:
    2896 (Human); 14824 (Mouse)
  • Alternate Names:
    Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
    Biochem Biophys Res Commun, 2012;430(1):419-24.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Human tumors instigate granulin-expressing hematopoietic cells that promote malignancy by activating stromal fibroblasts in mice.
    Authors: Elkabets M, Gifford AM, Scheel C, Nilsson B, Reinhardt F, Bray MA, Carpenter AE, Jirstrom K, Magnusson K, Ebert BL, Ponten F, Weinberg RA, McAllister SS
    J. Clin. Invest., 2011;121(2):784-99.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
Cell and Tissue Staining Kits
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Normal Rat IgG Control

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Rat IgG2A Isotype Control

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Secondary Antibodies
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rat IgG Biotinylated Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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