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Mouse Protein S/PROS1 Antibody

Catalog #: MAB4976
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4976
MAB4976-SP

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Detection of Mouse Protein S/PROS1 by Western Blot.
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Citations (2)
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Mouse Protein S/PROS1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Protein S/PROS1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protein S is observed.
Source
Monoclonal Rat IgG2A Clone # 818002
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Protein S/PROS1
Met1-Arg669
Accession # Q08761
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Protein S/PROS1 antibody by Western Blot. View Larger

Detection of Mouse Protein S/PROS1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4976) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein S/PROS1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein S/PROS1

Protein S (PROS1) is a 70-75 kDa plasma glycoprotein member of the vitamin K dependent family of proteins.  It is synthesized by hepatocytes, Sertoli cells, macrophages, vascular smooth muscle cells and endothelium.  Protein S is a non-enzymatic cofactor for activated protein C and participates in the degradation of coagulation factors FVa and FVIIIa.  It also binds to Axl on smooth muscle, likely initiating mitosis.  Protein S circulates free in plasma and, unlike Protein S in human and rat, does not complex with C4BP.  Mouse Protein S precursor is 675 amino acids (aa) in length.  It contains a signal sequence (aa 1-24), a cleavable prosegment (aa 25-41) and a 634 aa mature region.  The mature molecule contains one Gla region (aa 42-87), four EGF-like domains (aa 117-283) and two laminin G-like regions (aa 299-665).  Over aa 1-169 of the mature molecule, mouse Protein S shares 80% and 88% aa identity with human and rat Protein S, respectively.

Entrez Gene IDs
5627 (Human); 19128 (Mouse); 81750 (Rat)
Alternate Names
EC 3.4.21; PROS; PROS1; protein S (alpha); Protein S; protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; vitamin K-dependent plasma protein S; vitamin K-dependent protein S

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Citations for Mouse Protein S/PROS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Stromal changes in the aged lung induce an emergence from melanoma dormancy
    Authors: ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna
    Nature, 2022-06-01;606(7913):396-405.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Anticoagulant Protein S Targets the Factor IXa Heparin-Binding Exosite to Prevent Thrombosis
    Authors: WE Plautz, VS Sekhar Pil, BC Cooley, R Chattopadh, PR Westmark, T Getz, D Paul, W Bergmeier, JP Sheehan, R Majumder
    Arterioscler. Thromb. Vasc. Biol., 2018-02-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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