|Detection of Mouse Protein S/PROS1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4976) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein S/PROS1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Protein S (PROS1) is a 70-75 kDa plasma glycoprotein member of the vitamin K dependent family of proteins. It is synthesized by hepatocytes, Sertoli cells, macrophages, vascular smooth muscle cells and endothelium. Protein S is a non-enzymatic cofactor for activated protein C and participates in the degradation of coagulation factors FVa and FVIIIa. It also binds to Axl on smooth muscle, likely initiating mitosis. Protein S circulates free in plasma and, unlike Protein S in human and rat, does not complex with C4BP. Mouse Protein S precursor is 675 amino acids (aa) in length. It contains a signal sequence (aa 1-24), a cleavable prosegment (aa 25-41) and a 634 aa mature region. The mature molecule contains one Gla region (aa 42-87), four EGF-like domains (aa 117-283) and two laminin G-like regions (aa 299-665). Over aa 1-169 of the mature molecule, mouse Protein S shares 80% and 88% aa identity with human and rat Protein S, respectively.
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