Detects recombinant mouse (rm) R‑Spondin 1 in ELISA. In ELISA, no cross-reactivity with rmR-Spondin 3 and rhR-Spondin 1 is observed.
Monoclonal Rat IgG2B Clone # 852010
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse R‑Spondin 1 Met1-Gln265 Accession # Q9Z132
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Measured by its ability to neutralize R‑Spondin 1-induced beta -catenin response in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 0.4-2 μg/mL in the presence of 500 ng/mL Recombinant Mouse R‑Spondin 1 and 5 ng/mL Recombinant Mouse Wnt-3a.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
beta -Catenin Response Induced by R-Spondin 1 and Neutralization by Mouse R‑Spondin 1 Antibody. Recombinant Mouse R‑Spondin 1 (Catalog # 7150-RS) co-stimulates beta -catenin response in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line). beta -Catenin Response elicited by Recombinant Mouse R‑Spondin 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse R‑Spondin 1 Monoclonal Antibody (Catalog # MAB3474) in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ND50 is typically 0.4‑2 μg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: R-Spondin 1
RSPO-1 (roof plate-specific spondin; also R-Spondin 1 and cristin3) is a 39 kDa, heparin-binding member of the Cristin/R-Spondin family of proteins. These molecules apparently are either retained intracellularly or secreted, where they activate the frizzled-LRP receptor pathway. Mature human RSPO-1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. A presumably glycosylated, 30 kDa, 193 aa C-terminally truncated form is secreted and interacts with heparin. The secreted form of human RSPO-1 is 93% aa identical to secreted mouse RSPO-1.
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