beta -Catenin Response Induced by R-Spondin 1 and Neutralization by Mouse|
R‑Spondin 1 Antibody. Recombinant Mouse R‑Spondin 1 (Catalog # 7150-RS) co-stimulates beta -catenin response in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line). beta -Catenin Response elicited by Recombinant Mouse R‑Spondin 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse R‑Spondin 1 Monoclonal Antibody (Catalog # MAB3474) in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ND50 is typically 0.4‑2 μg/mL.
RSPO-1 (roof plate-specific spondin; also R-Spondin 1 and cristin3) is a 39 kDa, heparin-binding member of the Cristin/R-Spondin family of proteins. These molecules apparently are either retained intracellularly or secreted, where they activate the frizzled-LRP receptor pathway. Mature human RSPO-1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. A presumably glycosylated, 30 kDa, 193 aa C-terminally truncated form is secreted and interacts with heparin. The secreted form of human RSPO-1 is 93% aa identical to secreted mouse RSPO-1.
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