Mouse/Rat CD25/IL-2 R alpha PE-conjugated Antibody

  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse CD25/IL-2 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human CD25/IL-2 R alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha
    Glu22-Lys236
    Accession # Q544I2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of CD25/IL-2 R alpha in Rat CD4+ T cells by Flow Cytometry.

Rat CD4+ T cells purified with the Rat CD4+ T cell Enrichment Column (Catalog # RCD4C-1000) were stained with Goat Anti-Mouse/Rat CD25/IL‑2 R alpha PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6750P) and Anti-Rat CD4 APC-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Detection of CD25/IL-2 R alpha in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD25/IL‑2 R alpha PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6750P) and Anti-Mouse CD4 APC-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2 R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12-14).

  • References:
    1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
    2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
    3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
    4. Miller, J. et al. (1985) J. Immunol. 134:4212.
    5. Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
    6. Hatakeyama, M. et al. (1989) Science 244:551.
    7. Takeshita, T. et al. (1992) Science 257:379.
    8. Wang, X. et al. (2005) Science 310:1159.
    9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
    10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
    11. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
    12. Willerford, D.M. et al. (1995) Immunity 3:521.
    13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
    14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
  • Alternate Names:
    CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 4
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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