Mouse/Rat FGF-21 Quantikine ELISA Kit

  (33 citations)     
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Assay Procedure
Citations (33)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    13.4 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse and rat FGF-21
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat FGF-21 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FGF-21 in mouse or rat cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse FGF-21 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse FGF-21. Results obtained using natural mouse or rat FGF-21 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF-21.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 84.4 226 754 94.5 231 745
Standard Deviation 5.24 9.6 19.7 6.61 10.8 31
CV% 6.2 4.2 2.6 7 4.7 4.2

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 118 317 1032 120 302 977
Standard Deviation 7.35 13.5 25.2 8.91 18 65.9
CV% 6.2 4.3 2.4 7.4 6 6.7

Recovery

The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Samples (n=6) 96 88-101
Mouse EDTA Plasma (n=4) 97 86-104
Mouse Heparin Plasma (n=4) 93 83-101
Mouse Serum (n=4) 95 83-103
Rat Cell Culture Samples (n=2) 91 87-95
Rat EDTA Plasma (n=4) 101 93-119
Rat Heparin Plasma (n=4) 97 94-104
Rat Serum (n=4) 100 93-108
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.
 FGF-21 [HRP]
 FGF-21 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF-21
FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
  • Long Name:
    Fibroblast Growth Factor 21
  • Entrez Gene IDs:
    26291 (Human); 56636 (Mouse)
  • Alternate Names:
    FGF21; FGF-21; fibroblast growth factor 21
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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Species
Sample Type
  1. Fibroblast growth factor 21 (FGF21) is robustly induced by ethanol and has a protective role in ethanol associated liver injury
    Authors: BN Desai, G Singhal, M Watanabe, D Stevanovic, T Lundasen, FM Fisher, ML Mather, HG Vardeh, N Douris, AC Adams, IA Nasser, GA FitzGerald, JS Flier, C Skarke, E Maratos-Fl
    Mol Metab, 2017;6(11):1395-1406.
  2. Loss of Hepatic Mitochondrial Long-Chain Fatty Acid Oxidation Confers Resistance to Diet-Induced Obesity and Glucose Intolerance
    Authors: J Lee, J Choi, ES Selen Alpe, L Zhao, T Hartung, S Scafidi, RC Riddle, MJ Wolfgang
    Cell Rep, 2017;20(3):655-667.
    Species: Mouse
    Sample Type: Serum
  3. Repletion of branched chain amino acids reverses mTORC1 signaling but not improved metabolism during dietary protein dilution
    Authors: A Maida, JSK Chan, KA Sjøberg, A Zota, D Schmoll, B Kiens, S Herzig, AJ Rose
    Mol Metab, 2017;6(8):873-881.
    Species: Mouse
    Sample Type: Serum
  4. Lipophagy maintains energy homeostasis in the kidney proximal tubule during prolonged starvation
    Authors: S Minami, T Yamamoto, Y Takabatake, A Takahashi, T Namba, J Matsuda, T Kimura, JY Kaimori, I Matsui, T Hamano, H Takeda, M Takahashi, Y Izumi, T Bamba, T Matsusaka, F Niimura, Y Isaka
    Autophagy, 2017;0(0):0.
    Species: Mouse
    Sample Type: Plasma
  5. Peripherally derived FGF21 promotes remyelination in the central nervous system
    Authors: M Kuroda, R Muramatsu, N Maedera, Y Koyama, M Hamaguchi, H Fujimura, M Yoshida, M Konishi, N Itoh, H Mochizuki, T Yamashita
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: Serum
  6. Stromal cell cadherin-11 regulates adipose tissue inflammation and diabetes
    Authors: SK Chang, AC Kohlgruber, F Mizoguchi, X Michelet, BJ Wolf, K Wei, PY Lee, L Lynch, D Duquette, V Ceperuelo-, AS Banks, MB Brenner
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: Serum
  7. Loss of hepatic DEPTOR alters the metabolic transition to fasting
    Authors: A Caron, M Mouchiroud, N Gautier, SM Labbé, R Villot, L Turcotte, B Secco, G Lamoureux, M Shum, Y Gélinas, A Marette, D Richard, DM Sabatini, M Laplante
    Mol Metab, 2017;6(5):447-458.
    Species: Mouse
    Sample Type: Plasma
  8. Fgf21 regulates T-cell development in the neonatal and juvenile thymus
    Authors: Y Nakayama, Y Masuda, H Ohta, T Tanaka, M Washida, YI Nabeshima, A Miyake, N Itoh, M Konishi
    Sci Rep, 2017;7(1):330.
    Species: Mouse
    Sample Type: Serum
  9. Erythropoietin (EPO) ameliorates obesity and glucose homeostasis by promoting thermogenesis and endocrine function of classical brown adipose tissue (BAT) in diet-induced obese mice
    Authors: K Kodo, S Sugimoto, H Nakajima, J Mori, I Itoh, S Fukuhara, K Shigehara, T Nishikawa, K Kosaka, H Hosoi
    PLoS ONE, 2017;12(3):e0173661.
    Species: Mouse
    Sample Type: Plasma
  10. Preserving of Postnatal Leptin Signaling in Obesity-Resistant Lou/C Rats following a Perinatal High-Fat Diet
    PLoS ONE, 2016;11(9):e0162517.
    Species: Rat
    Sample Type: Plasma
  11. mTORC1 is Required for Brown Adipose Tissue Recruitment and Metabolic Adaptation to Cold
    Sci Rep, 2016;6(0):37223.
    Species: Mouse
    Sample Type: Plasma
  12. CREB3L3 controls fatty acid oxidation and ketogenesis in synergy with PPAR?
    Sci Rep, 2016;6(0):39182.
    Species: Mouse
    Sample Type: Plasma
  13. The Orphan Nuclear Receptor ERR? Regulates Hepatic CB1 Receptor-Mediated Fibroblast Growth Factor 21 Gene Expression
    PLoS ONE, 2016;11(7):e0159425.
    Species: Mouse
    Sample Type: Serum
  14. Hyperlipidemia and hepatitis in liver-specific CREB3L3 knockout mice generated using a one-step CRISPR/Cas9 system
    Authors: Yoshimi Nakagawa
    Sci Rep, 2016;6(0):27857.
    Species: Mouse
    Sample Type: Plasma
  15. Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction
    Authors: Jong-Ho Kim
    PLoS ONE, 2016;11(6):e0158067.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  16. CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis
    Authors: Jong-Gil Park
    Sci Rep, 2016;6(0):27938.
    Species: Mouse
    Sample Type: Plasma
  17. Single ingestion of soy ?-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects
    Authors: Tsutomu Hashidume
    Sci Rep, 2016;6(0):28183.
    Species: Mouse
    Sample Type: Serum
  18. Hepatic CREB3L3 controls whole-body energy homeostasis and improves obesity and diabetes.
    Authors: Nakagawa Y, Satoh A, Yabe S, Furusawa M, Tokushige N, Tezuka H, Mikami M, Iwata W, Shingyouchi A, Matsuzaka T, Kiwata S, Fujimoto Y, Shimizu H, Danno H, Yamamoto T, Ishii K, Karasawa T, Takeuchi Y, Iwasaki H, Shimada M, Kawakami Y, Urayama O, Sone H, Takekoshi K, Kobayashi K, Yatoh S, Takahashi A, Yahagi N, Suzuki H, Yamada N, Shimano H
    Endocrinology, 2015;155(12):4706-19.
    Species: Mouse
    Sample Type: Plasma
  19. A muscle-liver-fat signalling axis is essential for central control of adaptive adipose remodelling.
    Authors: Shimizu N, Maruyama T, Yoshikawa N, Matsumiya R, Ma Y, Ito N, Tasaka Y, Kuribara-Souta A, Miyata K, Oike Y, Berger S, Schutz G, Takeda S, Tanaka H
    Nat Commun, 2015;6(0):6693.
    Species: Mouse
    Sample Type: Plasma
  20. Hepatic mitogen-activated protein kinase phosphatase 1 selectively regulates glucose metabolism and energy homeostasis.
    Authors: Lawan A, Zhang L, Gatzke F, Min K, Jurczak M, Al-Mutairi M, Richter P, Camporez J, Couvillon A, Pesta D, Roth Flach R, Shulman G, Bennett A
    Mol Cell Biol, 2015;35(1):26-40.
    Species: Mouse
    Sample Type: Serum
  21. Long-term low carbohydrate diet leads to deleterious metabolic manifestations in diabetic mice.
    Authors: Handa K, Inukai K, Onuma H, Kudo A, Nakagawa F, Tsugawa K, Kitahara A, Moriya R, Takahashi K, Sumitani Y, Hosaka T, Kawakami H, Oyadomari S, Ishida H
    PLoS ONE, 2014;9(8):e104948.
    Species: Mouse
    Sample Type: Serum
  22. Activation of AMPKalpha2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity.
    Authors: Ost M, Werner F, Dokas J, Klaus S, Voigt A
    PLoS ONE, 2014;9(4):e94689.
    Species: Mouse
    Sample Type: Plasma
  23. Effective treatment of mitochondrial myopathy by nicotinamide riboside, a vitamin B3.
    Authors: Khan N, Auranen M, Paetau I, Pirinen E, Euro L, Forsstrom S, Pasila L, Velagapudi V, Carroll C, Auwerx J, Suomalainen A
    EMBO Mol Med, 2014;6(6):721-31.
    Species: Mouse
    Sample Type: Serum
  24. A serum component mediates food restriction-induced growth attenuation.
    Authors: Pando, Rakefet, Shtaif, Biana, Phillip, Moshe, Gat-Yablonski, Galia
    Endocrinology, 2014;155(3):932-40.
    Species: Rat
    Sample Type: Serum
  25. Fibroblast growth factor 21 and thyroid hormone show mutual regulatory dependency but have independent actions in vivo.
    Authors: Domouzoglou, Eleni M, Fisher, Ffolliot, Astapova, Inna, Fox, Elliott, Kharitonenkov, Alexei, Flier, Jeffrey, Hollenberg, Anthony, Maratos-Flier, Elefther
    Endocrinology, 2014;155(5):2031-40.
    Species: Mouse
    Sample Type: Serum
  26. Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart.
    Authors: Dogan S, Pujol C, Maiti P, Kukat A, Wang S, Hermans S, Senft K, Wibom R, Rugarli E, Trifunovic A
    Cell Metab, 2014;19(3):458-69.
    Species: Mouse
    Sample Type: Serum
  27. Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on hormones of energy balance in a TCDD-sensitive and a TCDD-resistant rat strain.
    Authors: Linden J, Lensu S, Pohjanvirta R
    Int J Mol Sci, 2014;15(8):13938-66.
    Species: Rat
    Sample Type: Serum
  28. Skeletal muscle mitochondrial uncoupling drives endocrine cross-talk through the induction of FGF21 as a myokine.
    Authors: Keipert S, Ost M, Johann K, Imber F, Jastroch M, van Schothorst E, Keijer J, Klaus S
    Am J Physiol Endocrinol Metab, 2014;306(5):E469-82.
    Species: Mouse
    Sample Type: Plasma
  29. Retinoic acid receptor beta stimulates hepatic induction of fibroblast growth factor 21 to promote fatty acid oxidation and control whole-body energy homeostasis in mice.
    Authors: Li Y, Wong K, Walsh K, Gao B, Zang M
    J Biol Chem, 2013;288(15):10490-504.
    Species: Mouse
    Sample Type: Plasma
  30. Activation of the farnesoid x receptor induces hepatic expression and secretion of fibroblast growth factor 21.
    Authors: Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB
    J. Biol. Chem., 2012;287(30):25123-38.
    Species: Rat
    Sample Type: Plasma
  31. Somatic progenitor cell vulnerability to mitochondrial DNA mutagenesis underlies progeroid phenotypes in Polg mutator mice.
    Authors: Ahlqvist KJ, Hamalainen RH, Yatsuga S, Uutela M, Terzioglu M, Gotz A, Forsstrom S, Salven P, Angers-Loustau A, Kopra OH, Tyynismaa H, Larsson NG, Wartiovaara K, Prolla T, Trifunovic A, Suomalainen A
    Cell Metab., 2012;15(1):100-9.
    Species: Mouse
    Sample Type: Serum
  32. Fasting-induced FGF21 is repressed by LXR activation via recruitment of an HDAC3 corepressor complex in mice.
    Authors: Archer A, Venteclef N, Mode A, Pedrelli M, Gabbi C, Clement K, Parini P, Gustafsson J, Korach-Andre M
    Mol Endocrinol, 2012;26(12):1980-90.
    Species: Mouse
    Sample Type: Serum
  33. Paradoxical regulation of human FGF21 by both fasting and feeding signals: is FGF21 a nutritional adaptation factor?
    Authors: Uebanso T, Taketani Y, Yamamoto H, Amo K, Ominami H, Arai H, Takei Y, Masuda M, Tanimura A, Harada N, Yamanaka-Okumura H, Takeda E
    PLoS ONE, 2011;6(8):e22976.
    Species: Human
    Sample Type: Cell Culture Supernates
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