FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
R&D Systems | Catalog # MFB00
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (10 µL), Serum (50 µL), EDTA Plasma (50 µL)
Sensitivity
3.68 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma)
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Product Summary for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
The Quantikine Mouse/Rat FGF basic Immunoassay is a 4.5 hour solid phase ELISA
designed to measure mouse or rat FGF basic in cell culture supernates, tissue lysates, serum, and plasma. It contains E.
coli-expressed
recombinant rat FGF basic and antibodies raised against the recombinant
factor. This immunoassay has been shown to quantitate the recombinant
factor accurately. Results obtained using natural FGF basic showed
dose-response curves that were parallel to the standard curves obtained
using the recombinant kit standards. These results indicate that this
kit can be used to determine relative mass values for natural FGF basic.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse, Rat
Specificity
Natural and recombinant FGF basic. This assay cross-reacts 100% with Bovine FGF basic, recombinant Human FGF basic is detectable.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Serum, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 49.5 | 122 | 394 | 45.0 | 104 | 382 |
| Standard Deviation | 1.17 | 2.7 | 12.0 | 3.80 | 5.03 | 20.5 |
| CV% | 2.4 | 2.2 | 3.1 | 8.4 | 4.8 | 5.4 |
Recovery for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 101 | 91-110 |
| EDTA Plasma (n=4) | 97 | 84-110 |
| Serum (n=4) | 95 | 84-110 |
| Tissue Lysates (n=4) | 97 | 80-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FGF basic were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
Mouse/Rat FGF basic ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF basic/FGF2/bFGF
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
Additional FGF basic/FGF2/bFGF Products
Product Documents for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
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Protocols
View specific protocols for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit (MFB00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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