Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
PMG100
SMG100
MG100
Mouse/Rat IGF-I ELISA Standard Curve
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Product Details
Procedure
Citations (107)
FAQs
Supplemental Products
Reviews (4)

Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Tissue Homogenates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
8.4 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat IGF-I
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat IGF-I Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IGF-I in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse IGF-I and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse and rat IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat IGF-I.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 35 35 35
Mean 82 269 921 86 256 863
Standard Deviation 4.6 8.9 37.7 7.8 11.8 37.4
CV% 5.6 3.3 4.1 9.1 4.6 4.3

Recovery

The recovery of mouse/rat IGF-I spiked to three levels throughout the range of the assay in the cell culture supernate samples was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=7) 98 89-111
Rat Cell Culture Supernates (n=6) 98 82-108

Linearity

To assess the linearity of the assay, samples containing mouse or rat IGF-I in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse/Rat IGF-I ELISA Linearity
Mouse/Rat IGF-I ELISA Linearity

Data Examples

Mouse/Rat IGF-I ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I/IGF-1

Insulin-like Growth Factor 1 (IGF-1), also known as somatomedin C, is a member of the insulin superfamily. It was originally discovered as a mediator of growth hormone actions on somatic cell growth, but has also been shown to be an important regulator of cell metabolism, differentiation and survival. IGF-1 is synthesized as a preproprotein that is proteolytically cleaved to generate the mature protein linked by three disulfide bonds. Mature IGF-1 is highly conserved among large mammals, with 100% sequence identity between the human, bovine, porcine, equine, and canine proteins. 


Mature mouse IGF-1 is a non-glycosylated, 70 amino acid (aa) secreted polypeptide that is derived from either a 153 aa or a 159 aa preproprotein. It shares 99% and 94% aa sequence identity with rat and human IGF-1, respectively. IGF-1 is synthesized in the liver and other tissues. It is found in blood and other body fluids as a complex with specific high affinity IGF binding proteins (IGFBP-1 to -6). The IGFBPs are expressed in specific patterns during development. They are modulators of IGF actions, which control IGF bioavailability to specific cell-surface receptors. Their functions are further regulated by IGFBP proteases, which proteolytically cleave the IGFBPs to lower the affinity with which they bind IGFs and increase IGF bioavailability. Some IGFBPs also have IGF-Independent effects on cell functions. IGF-Independent circulates primarily as a ternary complex with IGFBP-3 or IGFBP-5 and the acid-labile subunit (ALS). Some IGF-1 is also present in binary complexes with other IGFBPs. Whereas the ternary complexes are generally restricted to the vasculature, the binary complexes freely enter the tissues. 

IGF-1 actions are mediated by two ubiquitously expressed receptor tyrosine kinases: IGF-I R and Insulin R/CD220. IGF-I R and Insulin R are disulfide-linked heterotetrameric complexes that consist of two alpha and two beta subunits. For both of these receptors, the prepro proteins are cleaved to produce extracellular alpha subunits which contain a cysteine-rich region and ligand-binding fibronectin type III (FN-III) domains, and beta subunits which contain an extracellular FN-III domain, transmembrane, and cytoplasmic tyrosine kinase domains. A hybrid complex containing one IGF-I R and one Insulin R also serves as a functional high affinity receptor for IGF-1. IGF-I R-Insulin R hybrids respond primarily to IGF-1, potentially downregulating the cellular response to Insulin. IGF signaling is also modulated by IGF binding proteins and the scavenger receptor, IGF-I I R.

Long Name:
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs:
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names:
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

107 Citations: Showing 1 - 10
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  1. Ketogenic diet induces skeletal muscle atrophy via reducing muscle protein synthesis and possibly activating proteolysis in mice
    Authors: R Nakao, T Abe, S Yamamoto, K Oishi
    Sci Rep, 2019;9(1):19652.
    Species: Mouse
    Sample Types: Plasma
  2. Implantable multireservoir device with stimulus-responsive membrane for on-demand and pulsatile delivery of growth hormone
    Authors: SH Lee, H Piao, YC Cho, SN Kim, G Choi, CR Kim, HB Ji, CG Park, C Lee, CI Shin, WG Koh, YB Choy, JH Choy
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Rat
    Sample Types: Plasma
  3. Pregnancy-Associated Plasma Protein-A Accelerates Atherosclerosis by Regulating Reverse Cholesterol Transport and Inflammation
    Authors: SL Tang, ZW Zhao, SM Liu, G Wang, XH Yu, J Zou, SQ Wang, XY Dai, MG Fu, XL Zheng, DW Zhang, H Fu, CK Tang
    Circ. J., 2019;0(0):.
    Species: Mouse
    Sample Types: Serum
  4. Topical essential fatty acid oil on wounds: Local and systemic effects
    Authors: BG Lania, J Morari, AR Almeida, MND Silva, G Vieira-Dam, KA Lins, CL César, LA Velloso, NB Maia, ML Cintra, PENF Velho
    PLoS ONE, 2019;14(1):e0210059.
    Species: Mouse
    Sample Types: Serum
  5. Growth Hormone Improves Nerve Regeneration, Muscle Re-innervation, and Functional Outcomes After Chronic Denervation Injury
    Authors: J Lopez, A Quan, J Budihardjo, S Xiang, H Wang, Kiron Kosh, C Cashman, WPA Lee, A Hoke, S Tuffaha, G Brandacher
    Sci Rep, 2019;9(1):3117.
    Species: Rat
    Sample Types: Serum
  6. Fibroblast growth factor 21 coordinates adiponectin to mediate the beneficial effects of low-protein diet on primordial follicle reserve
    Authors: Y Zhuo, L Hua, B Feng, X Jiang, J Li, D Jiang, X Huang, Y Zhu, Z Li, L Yan, C Jin, L Che, Z Fang, Y Lin, S Xu, J Li, Wu
    EBioMedicine, 2019;41(0):623-635.
    Species: Mouse
    Sample Types: Serum
  7. Potassium chloride released from contracting skeletal muscle may stimulate development of its hypertrophy
    Authors: IV Kravchenko, VA Furalyov, VO Popov
    Biochem Biophys Rep, 2019;18(0):100627.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. A DNMT3A PWWP mutation leads to methylation of bivalent chromatin and growth retardation in mice
    Authors: G Sendžikait, CW Hanna, KR Stewart-Mo, E Ivanova, G Kelsey
    Nat Commun, 2019;10(1):1884.
    Species: Mouse
    Sample Types: Serum
  9. Hormone replacement therapy attenuates hearing loss: Mechanisms involving estrogen and the IGF-1 pathway
    Authors: TT Williamson, B Ding, X Zhu, RD Frisina
    Aging Cell, 2019;0(0):e12939.
    Species: Mouse
    Sample Types: Serum
  10. Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model
    Authors: CC Hsiao, CC Lin, YS Hou, JY Ko, CJ Wang
    Int J Mol Sci, 2019;20(19):.
    Species: Rat
    Sample Types: Tissue Lysates
  11. Food deprivation during active phase induces skeletal muscle atrophy via IGF-1 reduction in mice
    Authors: T Abe, R Kazama, H Okauchi, K Oishi
    Arch. Biochem. Biophys., 2019;0(0):108160.
    Species: Mouse
    Sample Types: Plasma
  12. Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure
    Authors: YF Mahran, AM Badr, A Aldosari, R Bin-Zaid, HN Alotaibi
    Oxid Med Cell Longev, 2019;2019(0):3173745.
    Species: Rat
    Sample Types: Serum
  13. Potentiation of Glucagon-Like Peptide-2 Dynamics by Methotrexate Administration in Rat Small Intestine
    Authors: M Machida, S Shiga, T Machida, M Ohno, K Iizuka, M Hirafuji
    Biol. Pharm. Bull., 2019;42(10):1733-1740.
    Species: Rat
    Sample Types: Plasma
  14. Mesenchymal stem cell-derived conditioned medium attenuate angiotensin II-induced aortic aneurysm growth by modulating macrophage polarization
    Authors: YZ Zhou, Z Cheng, Y Wu, QY Wu, XB Liao, Y Zhao, JM Li, XM Zhou, XM Fu
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Effects of Intestinal Microbiota on Brain Development in Humanized Gnotobiotic Mice
    Authors: J Lu, L Lu, Y Yu, J Cluette-Br, CR Martin, EC Claud
    Sci Rep, 2018;8(1):5443.
    Species: Mouse
    Sample Types: Serum
  16. Myeloid p38? signaling promotes intestinal IGF-1 production and inflammation-associated tumorigenesis
    Authors: C Youssif, M Cubillos-R, M Comalada, E Llonch, C Perna, N Djouder, AR Nebreda
    EMBO Mol Med, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. EGFR controls bone development by negatively regulating mTOR-signaling during osteoblast differentiation
    Authors: M Linder, M Hecking, E Glitzner, K Zwerina, M Holcmann, L Bakiri, MG Ruocco, J Tuckermann, G Schett, EF Wagner, M Sibilia
    Cell Death Differ., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Methionine Restriction Extends Lifespan in Progeroid Mice and Alters Lipid and Bile Acid Metabolism
    Authors: C Bárcena, PM Quirós, S Durand, P Mayoral, F Rodríguez, XM Caravia, G Mariño, C Garabaya, MT Fernández-, G Kroemer, JMP Freije, C López-Otín
    Cell Rep, 2018;24(9):2392-2403.
    Species: Mouse
    Sample Types: Plasma
  19. Enhanced Pulsatile Growth Hormone Secretion and Altered Metabolic Hormones by in Vivo Hexarelin Treatment in Streptozotocin-Induced Diabetic Rats
    Authors: X Zhang, JK Yang, C Chen
    Int J Mol Sci, 2018;19(10):.
    Species: Rat
    Sample Types: Serum
  20. Therapeutic effects of a mesenchymal stem cell?based insulin?like growth factor?1/enhanced green fluorescent protein dual gene sorting system in a myocardial infarction rat model
    Authors: S Jung, JH Kim, C Yim, M Lee, HJ Kang, D Choi
    Mol Med Rep, 2018;0(0):.
    Species: Rat
    Sample Types: Transfected Whole Cells
  21. Novel phytopeptide osmotin mimics preventive effects of adiponectin on vascular inflammation and atherosclerosis
    Authors: Y Takahashi, R Watanabe, Y Sato, N Ozawa, M Kojima, K Watanabe-K, R Shirai, K Sato, T Hirano, T Watanabe
    Metab. Clin. Exp., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  22. Late-life targeting of the IGF-1 receptor improves healthspan and lifespan in female mice
    Authors: K Mao, GF Quipildor, T Tabrizian, A Novaj, F Guan, RO Walters, F Delahaye, GB Hubbard, Y Ikeno, K Ejima, P Li, DB Allison, H Salimi-Moo, PJ Beltran, P Cohen, N Barzilai, DM Huffman
    Nat Commun, 2018;9(1):2394.
    Species: Mouse
    Sample Types: Plasma
  23. Transplant of insulin-like growth factor-1 expressing bone marrow stem cells improves functional regeneration of injured rat uterus by NF-?B pathway
    Authors: L Wang, M Yang, M Jin, Y Wu, T Zheng, S Gu, X Hua
    J. Cell. Mol. Med., 2018;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  24. Growth Hormone Improves Cognitive Function After Experimental Stroke
    Authors: LK Ong, WZ Chow, C TeBay, M Kluge, G Pietrogran, K Zalewska, P Crock, ND Åberg, A Bivard, SJ Johnson, FR Walker, M Nilsson, J Isgaard
    Stroke, 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
  25. Intermittent hypoxia suppression of growth hormone and insulin-like growth factor-I in the neonatal rat liver
    Authors: C Cai, T Ahmad, GB Valencia, JV Aranda, J Xu, KD Beharry
    Growth Horm. IGF Res., 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  26. The effect of the type of dietary protein on the development of ovarian cancer
    Authors: AAA Taha, M Koshiyama, N Matsumura, K Abiko, K Yamaguchi, J Hamanishi, T Baba, B Kharma, IH Mohamed, MM Ameen, SA Ismail, I Konishi, M Mandai
    Oncotarget, 2018;9(35):23987-23999.
    Species: Rat
    Sample Types: Serum
  27. The local cytokine and growth factor response to rhBMP-2 after spinal fusion
    Authors: JD Koerner, DZ Markova, GD Schroeder, BP Calio, A Shah, CW Brooks, AR Vaccaro, DG Anderson, CK Kepler
    Spine J, 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: ELISA (Standard)
  28. The Protective Effects of IGF-I against ?-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A
    Authors: D Aguado-Lle, S Canelles, LM Frago, JA Chowen, J Argente, E Arilla, V Barrios
    Neuroscience, 2018;374(0):104-118.
    Species: Rat
    Sample Types: Tissue Homogenates
  29. Supplementation of suckling rats with cow's milk induces hyperphagia and higher visceral adiposity in females at adulthood, but not in males
    Authors: VST Rodrigues, EG Moura, DN Bernardino, JC Carvalho, PN Soares, TC Peixoto, N Peixoto-Si, E Oliveira, PC Lisboa
    J. Nutr. Biochem., 2017;55(0):89-103.
    Species: Rat
    Sample Types: Serum
  30. Corticotrophin-releasing hormone and corticosterone impair development of preimplantation embryos by inducing oviductal cell apoptosis via activating the Fas system: an in vitro study
    Authors: XW Tan, CL Ji, LL Zheng, J Zhang, HJ Yuan, S Gong, J Zhu, JH Tan
    Hum. Reprod., 2017;0(0):1-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice
    Authors: L Luo, C Yan, Y Urata, AS Hasan, S Goto, CY Guo, S Zhang, TS Li
    Sci Rep, 2017;7(0):40959.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. IGF-1 has sexually dimorphic, pleiotropic, and time-dependent effects on healthspan, pathology, and lifespan
    Authors: NM Ashpole, S Logan, A Yabluchans, MC Mitschelen, H Yan, JA Farley, EL Hodges, Z Ungvari, A Csiszar, S Chen, C Georgescu, GB Hubbard, Y Ikeno, WE Sonntag
    Geroscience, 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  33. The type I insulin-like growth factor regulates the liver stromal response to metastatic colon carcinoma cells
    Authors: MC Fernandez, R Rayes, B Ham, N Wang, F Bourdeau, S Milette, M Lllemann, N Bird, A Majeed, J Xu, T Kisselova, P Brodt
    Oncotarget, 2017;8(32):52281-52293.
    Species: Mouse
    Sample Types: Serum
  34. Beneficial effect of combined treatment with octreotide and pasireotide in PCK rats, an orthologous model of human autosomal recessive polycystic kidney disease
    Authors: M Kugita, K Nishii, T Yamaguchi, A Suzuki, Y Yuzawa, S Horie, E Higashihar, S Nagao
    PLoS ONE, 2017;12(5):e0177934.
    Species: Rat
    Sample Types: Serum
  35. Changes of Cerebral and/or Peripheral Adenosine A? Receptor and IGF-I Concentrations under Extended Sleep Duration in Rats
    Authors: M Chennaoui, PJ Arnal, R Dorey, F Sauvet, S Ciret, T Gallopin, D Leger, C Drogou, D Gomez-Meri
    Int J Mol Sci, 2017;18(11):.
    Species: Rat
    Sample Types: Plasma
  36. Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)
    Authors: BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho
    PLoS ONE, 2017;12(12):e0188779.
    Species: Rat
    Sample Types: Serum
  37. Metformin inhibits the proliferation of benign prostatic epithelial cells
    Authors: Z Wang, X Xiao, R Ge, J Li, CW Johnson, C Rassoulian, AF Olumi
    PLoS ONE, 2017;12(3):e0173335.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Protective effect of maternal uteroplacental insufficiency on oxygen-induced retinopathy in offspring: removing bias of premature birth
    Authors: S Becker, H Wang, B Yu, R Brown, X Han, RH Lane, ME Hartnett
    Sci Rep, 2017;7(0):42301.
    Species: Rat
    Sample Types: Serum
  39. A novel 5HT3 receptor-IGF1 mechanism distinct from SSRI-induced antidepressant effects
    Authors: M Kondo, Y Koyama, Y Nakamura, S Shimada
    Mol. Psychiatry, 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  40. Uncoupling of Metabolic Health from Longevity through Genetic Alteration of Adipose Tissue Lipid-Binding Proteins
    Authors: KN Charles, MD Li, F Engin, AP Arruda, K Inouye, GS Hotamislig
    Cell Rep, 2017;21(2):393-402.
    Species: Mouse
    Sample Types: Serum
  41. Effects of growth hormone on cardiac remodeling and soleus muscle in rats with aortic stenosis-induced heart failure
    Authors: ARR Lima, LU Pagan, RL Damatto, MDM Cezar, C Bonomo, MJ Gomes, PF Martinez, DM Guizoni, DHS Campos, FC Damatto, K Okoshi, MP Okoshi
    Oncotarget, 2017;8(47):83009-83021.
    Species: Rat
    Sample Types: Serum
  42. Mosaic expression of Atrx in the mouse central nervous system causes memory deficits
    Authors: RJ Tamming, JR Siu, Y Jiang, MA Prado, F Beier, NG B?rub?
    Dis Model Mech, 2017;10(2):119-126.
    Species: Mouse
    Sample Types: Plasma
  43. Insulin-like growth factor 1 receptor regulates hypothermia during calorie restriction
    Authors: R Cintron-Co, M Sanchez-Al, W Nguyen, S Mori, R Gonzalez-R, T Lien, T Bartfai, S Aïd, JC François, M Holzenberg, B Conti
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  44. The histone deacetylase inhibitor, sodium butyrate, exhibits neuroprotective effects for ischemic stroke in middle-aged female rats
    J Neuroinflammation, 2016;13(1):300.
    Species: Rat
    Sample Types: Serum
  45. Deficiency in prohormone convertase PC1 impairs prohormone processing in Prader-Willi syndrome
    Authors: Lisa C Burnett
    J. Clin. Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: Plasma
  46. Positive Prehabilitative Effect of Intense Treadmill Exercise for Ameliorating Cancer Cachexia Symptoms in a Mouse Model
    J Cancer, 2016;7(15):2378-2387.
    Species: Mouse
    Sample Types: Plasma
  47. Cluster Differentiating 36 (CD36) Deficiency Attenuates Obesity-Associated Oxidative Stress in the Heart
    PLoS ONE, 2016;11(5):e0155611.
    Species: Mouse
    Sample Types: Plasma
  48. Fibroblast growth factor 21 has no direct role in regulating fertility in female mice
    Mol Metab, 2016;5(8):690-8.
    Species: Mouse
    Sample Types: Plasma
  49. Single chain Fc-dimer-human growth hormone fusion protein for improved drug delivery
    Authors: Li Zhou
    Biomaterials, 2016;117(0):24-31.
    Species: Mouse
    Sample Types: Plasma
  50. Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice
    PLoS ONE, 2016;11(5):e0152179.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. Single ingestion of soy ?-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects
    Authors: Tsutomu Hashidume
    Sci Rep, 2016;6(0):28183.
    Species: Mouse
    Sample Types: Serum
  52. Space radiation exposure persistently increased leptin and IGF1 in serum and activated leptin-IGF1 signaling axis in mouse intestine
    Sci Rep, 2016;6(0):31853.
    Species: Mouse
    Sample Types: Serum
  53. IGF-1 degradation by mouse mast cell protease 4 promotes cell death and adverse cardiac remodeling days after a myocardial infarction
    Authors: Thor Tejada
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Types: Serum
  54. First steps to define murine amniotic fluid stem cell microenvironment
    Sci Rep, 2016;6(0):37080.
    Species: Mouse
    Sample Types: Amniotic Fluid
  55. Microglial Kv1.3 Channels and P2Y12 Receptors Differentially Regulate Cytokine and Chemokine Release from Brain Slices of Young Adult and Aged Mice.
    Authors: Charolidi N, Schilling T, Eder C
    PLoS ONE, 2015;10(5):e0128463.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Naturally occurring p16(Ink4a)-positive cells shorten healthy lifespan.
    Authors: Baker D, Childs B, Durik M, Wijers M, Sieben C, Zhong J, Saltness R, Jeganathan K, Verzosa G, Pezeshki A, Khazaie K, Miller J, van Deursen J
    Nature, 2015;530(7589):184-9.
    Species: Mouse
    Sample Types: Serum
  57. Glucose intolerance in aging male IGFBP-3 transgenic mice: differential effects of human IGFBP-3 and its mutant IGFBP-3 devoid of IGF binding ability.
    Authors: Nguyen K, Yao X, Erickson A, Mishra S, Nyomba B
    Endocrinology, 2015;156(2):462-74.
    Species: Mouse
    Sample Types: Serum
  58. MeCP2 Affects Skeletal Muscle Growth and Morphology through Non Cell-Autonomous Mechanisms.
    Authors: Conti V, Gandaglia A, Galli F, Tirone M, Bellini E, Campana L, Kilstrup-Nielsen C, Rovere-Querini P, Brunelli S, Landsberger N
    PLoS ONE, 2015;10(6):e0130183.
    Species: Mouse
    Sample Types: Plasma
  59. Administration of saccharin to neonatal mice influences body composition of adult males and reduces body weight of females.
    Authors: Parlee, Sebastia, Simon, Becky R, Scheller, Erica L, Alejandro, Emilyn U, Learman, Brian S, Krishnan, Venkates, Bernal-Mizrachi, Ernesto, MacDougald, Ormond A
    Endocrinology, 2014;155(4):1313-26.
    Species: Mouse
    Sample Types: Serum
  60. Control of adipose tissue expandability in response to high fat diet by the insulin-like growth factor-binding protein-4.
    Authors: Gealekman O, Gurav K, Chouinard M, Straubhaar J, Thompson M, Malkani S, Hartigan C, Corvera S
    J Biol Chem, 2014;289(26):18327-38.
    Species: Mouse
    Sample Types: Tissue Homogenates
  61. The peroxisome proliferator-activated receptor (PPAR) alpha agonist fenofibrate suppresses chemically induced lung alveolar proliferative lesions in male obese hyperlipidemic mice.
    Authors: Kuno T, Hata K, Takamatsu M, Hara A, Hirose Y, Takahashi S, Imaida K, Tanaka T
    Int J Mol Sci, 2014;15(5):9160-72.
    Species: Mouse
    Sample Types: Plasma
  62. HGF signaling impacts severity of Pseudomonas aeruginosa keratitis.
    Authors: Jiang X, McClellan S, Barrett R, Foldenauer M, Hazlett L
    Invest Ophthalmol Vis Sci, 2014;55(4):2180-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
  63. Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling.
    Authors: Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B
    J Neurosci, 2014;34(38):12788-800.
    Species: Rat
    Sample Types: Serum
  64. Lipid profiling and transcriptomic analysis reveals a functional interplay between estradiol and growth hormone in liver.
    Authors: Fernandez-Perez L, Santana-Farre R, de Mirecki-Garrido M, Garcia I, Guerra B, Mateo-Diaz C, Iglesias-Gato D, Diaz-Chico J, Flores-Morales A, Diaz M
    PLoS ONE, 2014;9(5):e96305.
    Species: Rat
    Sample Types: Serum
  65. Bone resorption is regulated by cell-autonomous negative feedback loop of Stat5-Dusp axis in the osteoclast.
    Authors: Hirose J, Masuda H, Tokuyama N, Omata Y, Matsumoto T, Yasui T, Kadono Y, Hennighausen L, Tanaka S
    J Exp Med, 2014;211(1):153-63.
    Species: Mouse
    Sample Types: Serum
  66. The small-molecule BGP-15 protects against heart failure and atrial fibrillation in mice.
    Authors: Sapra, Geeta, Tham, Yow Keat, Cemerlang, Nelly, Matsumoto, Aya, Kiriazis, Helen, Bernardo, Bianca C, Henstridge, Darren C, Ooi, Jenny Y, Pretorius, Lynette, Boey, Esther J, Lim, Lydia, Sadoshima, Junichi, Meikle, Peter J, Mellet, Natalie, Woodcock, Elizabet, Marasco, Silvana, Ueyama, Tomomi, Du, Xiao-Jun, Febbraio, Mark A, McMullen, Julie R
    Nat Commun, 2014;5(0):5705.
    Species: Mouse
    Sample Types: Plasma
  67. The dwarf phenotype in GH240B mice, haploinsufficient for the autism candidate gene Neurobeachin, is caused by ectopic expression of recombinant human growth hormone.
    Authors: Nuytens, Kim, Tuand, Krizia, Fu, Quili, Stijnen, Pieter, Pruniau, Vincent, Meulemans, Sandra, Vankelecom, Hugo, Creemers, John W M
    PLoS ONE, 2014;9(10):e109598.
    Species: Mouse
    Sample Types: Plasma
  68. Skeletal muscle mitochondrial uncoupling drives endocrine cross-talk through the induction of FGF21 as a myokine.
    Authors: Keipert S, Ost M, Johann K, Imber F, Jastroch M, van Schothorst E, Keijer J, Klaus S
    Am J Physiol Endocrinol Metab, 2014;306(5):E469-82.
    Species: Mouse
    Sample Types: Plasma
  69. Calorie restriction decreases murine and human pancreatic tumor cell growth, nuclear factor-kappaB activation, and inflammation-related gene expression in an insulin-like growth factor-1-dependent manner.
    Authors: Harvey A, Lashinger L, Hays D, Harrison L, Lewis K, Fischer S, Hursting S
    PLoS ONE, 2014;9(5):e94151.
    Species: Mouse
    Sample Types: Serum
  70. The order of concurrent endurance and resistance exercise modifies mTOR signaling and protein synthesis in rat skeletal muscle.
    Authors: Ogasawara R, Sato K, Matsutani K, Nakazato K, Fujita S
    Am J Physiol Endocrinol Metab, 2014;306(10):E1155-62.
    Species: Rat
    Sample Types: Serum
  71. sigma1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue.
    Authors: Baltes J, Larsen J, Radhakrishnan K, Geumann C, Kratzke M, Petersen C, Schu P
    J Cell Sci, 2014;127(0):3477-87.
    Species: Mouse
    Sample Types: Serum
  72. Time-dependent expression profiles of microRNAs and mRNAs in rat milk whey.
    Authors: Izumi, Hirohisa, Kosaka, Nobuyosh, Shimizu, Takashi, Sekine, Kazunori, Ochiya, Takahiro, Takase, Mitsunor
    PLoS ONE, 2014;9(2):e88843.
    Species: Rat
    Sample Types: Serum
  73. Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
    Authors: Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet
    Am J Physiol Endocrinol Metab, 2014;306(8):E965-74.
    Species: Mouse
    Sample Types: Tissue Homogenates
  74. Long-term physiological alterations and recovery in a mouse model of separation associated with time-restricted feeding: a tool to study anorexia nervosa related consequences.
    Authors: Zgheib S, Mequinion M, Lucas S, Leterme D, Ghali O, Tolle V, Zizzari P, Bellefontaine N, Legroux-Gerot I, Hardouin P, Broux O, Viltart O, Chauveau C
    PLoS ONE, 2014;9(8):e103775.
    Species: Mouse
    Sample Types: Plasma
  75. Overlapping dose responses of spermatogenic and extragonadal testosterone actions jeopardize the principle of hormonal male contraception.
    Authors: Oduwole O, Vydra N, Wood N, Samanta L, Owen L, Keevil B, Donaldson M, Naresh K, Huhtaniemi I
    FASEB J, 2014;28(6):2566-76.
    Species: Mouse
    Sample Types: Serum
  76. IGF2 ameliorates amyloidosis, increases cholinergic marker expression and raises BMP9 and neurotrophin levels in the hippocampus of the APPswePS1dE9 Alzheimer's disease model mice.
    Authors: Mellott T, Pender S, Burke R, Langley E, Blusztajn J
    PLoS ONE, 2014;9(4):e94287.
    Species: Mouse
    Sample Types: Tissue Homogenates
  77. Sonic hedgehog gene therapy increases the ability of the dystrophic skeletal muscle to regenerate after injury.
    Authors: Piccioni A, Gaetani E, Palladino M, Gatto I, Smith R, Neri V, Marcantoni M, Giarretta I, Silver M, Straino S, Capogrossi M, Landolfi R, Pola R
    Gene Ther, 2014;21(4):413-21.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. Growth hormone secretion is correlated with neuromuscular innervation rather than motor neuron number in early-symptomatic male amyotrophic lateral sclerosis mice.
    Authors: Steyn, F J, Lee, K, Fogarty, M J, Veldhuis, J D, McCombe, P A, Bellingham, M C, Ngo, S T, Chen, C
    Endocrinology, 2013;154(12):4695-706.
    Species: Mouse
    Sample Types: Plasma
  79. BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer's disease.
    Authors: Burke R, Norman T, Haydar T, Slack B, Leeman S, Blusztajn J, Mellott T
    Proc Natl Acad Sci U S A, 2013;110(48):19567-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
  80. IGF-1 receptor deficiency in thyrocytes impairs thyroid hormone secretion and completely inhibits TSH-stimulated goiter.
    Authors: Ock, Sangmi, Ahn, Jihyun, Lee, Seok Hon, Kang, Hyun, Offermanns, Stefan, Ahn, Hwa Youn, Jo, Young Su, Shong, Minho, Cho, Bo Youn, Jo, Daewoong, Abel, E Dale, Lee, Tae Jin, Park, Woo Jin, Lee, In-Kyu, Kim, Jaetaek
    FASEB J, 2013;27(12):4899-908.
    Species: Mouse
    Sample Types: Serum
  81. SIRT1 regulates adaptive response of the growth hormone--insulin-like growth factor-I axis under fasting conditions in liver.
    Authors: Yamamoto, Masaaki, Iguchi, Genzo, Fukuoka, Hidenori, Suda, Kentaro, Bando, Hironori, Takahashi, Michiko, Nishizawa, Hitoshi, Seino, Susumu, Takahashi, Yutaka
    Proc Natl Acad Sci U S A, 2013;110(37):14948-53.
    Species: Mouse
    Sample Types: Serum
  82. Splicing factor SRSF3 is crucial for hepatocyte differentiation and metabolic function.
    Authors: Sen S, Jumaa H, Webster N
    Nat Commun, 2013;4(0):1336.
    Species: Mouse
    Sample Types: Serum
  83. IGF1 receptor signaling regulates adaptive radioprotection in glioma stem cells.
    Authors: Osuka S, Sampetrean O, Shimizu T, Saga I, Onishi N, Sugihara E, Okubo J, Fujita S, Takano S, Matsumura A, Saya H
    Stem Cells, 2013;31(4):627-40.
    Species: Mouse
    Sample Types: Cell Lysates
  84. The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function.
    Authors: Takehara, Yuji, Yabuuchi, Akiko, Ezoe, Kenji, Kuroda, Tomoko, Yamadera, Rie, Sano, Chiaki, Murata, Nana, Aida, Takuya, Nakama, Ken, Aono, Fumihito, Aoyama, Naoki, Kato, Keiich, Kato, Osamu
    Lab Invest, 2013;93(2):181-93.
    Species: Rat
    Sample Types: Cell Culture Supernates
  85. Lack of myotubularin (MTM1) leads to muscle hypotrophy through unbalanced regulation of the autophagy and ubiquitin-proteasome pathways.
    Authors: Al-Qusairi L, Prokic I, Amoasii L, Kretz C, Messaddeq N, Mandel J, Laporte J
    FASEB J, 2013;27(8):3384-94.
    Species: Mouse
    Sample Types: Plasma
  86. Effects of breastfeeding on body composition and maturational tempo in the rat.
    Authors: Crispel, Yonatan, Katz, Oren, Ben-Yosef, Dafna, Hochberg, Ze'ev
    BMC Med, 2013;11(0):114.
    Species: Rat
    Sample Types: Serum
  87. Fish oil slows prostate cancer xenograft growth relative to other dietary fats and is associated with decreased mitochondrial and insulin pathway gene expression.
    Authors: Lloyd J, Masko E, Wu C, Keenan M, Pilla D, Aronson W, Chi J, Freedland S
    Prostate Cancer Prostatic Dis, 2013;16(4):285-91.
    Species: Mouse
    Sample Types: Serum
  88. Cytosolic Malic Enzyme 1 (ME1) Mediates High Fat Diet-Induced Adiposity, Endocrine Profile, and Gastrointestinal Tract Proliferation-Associated Biomarkers in Male Mice.
    Authors: Al-Dwairi, Ahmed, Pabona, John Mar, Simmen, Rosalia, Simmen, Frank A
    PLoS ONE, 2012;7(10):e46716.
    Species: Mouse
    Sample Types: Serum
  89. Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway.
    Authors: Ma B, Zhang Q, Wu D, Wang YL, Hu YY, Cheng YP, Yang ZD, Zheng YY, Ying HJ
    Acta Pharmacol. Sin., 2012;33(4):479-89.
    Species: Rat
    Sample Types: Serum
  90. Human IGF Binding Protein-3 Overexpression Impairs Glucose Regulation in Mice via an Inhibition of Insulin Secretion.
    Authors: Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL
    Endocrinology, 2011;152(6):2184-96.
    Species: Mouse
    Sample Types: Plasma
  91. Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function.
    Authors: Sato H, Isogai Y, Masuda S
    J. Biol. Chem., 2011;286(13):11632-48.
    Species: Mouse
    Sample Types: Serum
  92. Peripheral SMN restoration is essential for long-term rescue of a severe spinal muscular atrophy mouse model.
    Authors: Hua Y, Sahashi K, Rigo F
    Nature, 2011;478(7367):123-6.
    Species: Mouse
    Sample Types: Serum
  93. Differential utilization of ketone bodies by neurons and glioma cell lines: a rationale for ketogenic diet as experimental glioma therapy.
    Authors: Maurer GD, Brucker DP, Bahr O, Harter PN, Hattingen E, Walenta S, Mueller-Klieser W, Steinbach JP, Rieger J
    BMC Cancer, 2011;11(0):315.
    Species: Mouse
    Sample Types: Serum
  94. Myocytic androgen receptor controls the strength but not the mass of limb muscles.
    Authors: Chambon C, Duteil D, Vignaud A, Ferry A, Messaddeq N, Malivindi R, Kato S, Chambon P, Metzger D
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(32):14327-32.
    Species: Mouse
    Sample Types: Plasma
  95. Rim2alpha determines docking and priming States in insulin granule exocytosis.
    Authors: Yasuda T, Shibasaki T, Minami K
    Cell Metab., 2010;12(2):117-29.
    Species: Mouse
    Sample Types: Serum
  96. Galectin-3 contributes to neonatal hypoxic-ischemic brain injury.
    Authors: Doverhag C, Hedtjarn M, Poirier F, Mallard C, Hagberg H, Karlsson A, Savman K
    Neurobiol. Dis., 2010;38(1):36-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
  97. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
    Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
    J. Neurochem., 2009;111(6):1409-24.
    Species: Mouse
    Sample Types: Tissue Homogenates
  98. Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.
    Authors: Kubota Y, Unoki H, Bujo H, Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M, Saito Y
    Biochem. Biophys. Res. Commun., 2008;368(1):81-7.
    Species: Mouse
    Sample Types: Plasma
  99. Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis.
    Authors: Paintlia AS, Paintlia MK, Singh AK, Singh I
    Mol. Pharmacol., 2008;73(5):1381-93.
    Species: Rat
    Sample Types: Serum
  100. Reduction in hypophyseal growth hormone and prolactin expression due to deficiency in ghrelin receptor signaling is associated with Pit-1 suppression: Relevance to the immune system.
    Authors: Yang H, Dixit VD, Patel K, Vandanmagsar B, Collins G, Sun Y, Smith RG, Taub DD
    Brain Behav. Immun., 2008;0(0):.
    Species: Mouse
    Sample Types: Serum
  101. Insulin-like growth factor-1 improves survival in sepsis via enhanced hepatic bacterial clearance.
    Authors: Ashare A, Nymon AB, Doerschug KC, Morrison JM, Monick MM, Hunninghake GW
    Am. J. Respir. Crit. Care Med., 2008;178(2):149-57.
    Species: Mouse
    Sample Types: Serum
  102. Modulation of melanocortin signaling ameliorates uremic cachexia.
    Authors: Cheung WW, Rosengren S, Boyle DL, Mak RH
    Kidney Int., 2008;74(2):180-6.
    Species: Mouse
    Sample Types: Tissue Homogenates
  103. Ovariectomy is protective against renal injury in the high-salt-fed older mRen2.Lewis rat.
    Authors: Yamaleyeva LM, Pendergrass KD, Pirro NT, Gallagher PE, Groban L, Chappell MC
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(4):H2064-71.
    Species: Rat
    Sample Types: Plasma
  104. Wheel running and fluoxetine antidepressant treatment have differential effects in the hippocampus and the spinal cord.
    Authors: Engesser-Cesar C, Anderson AJ, Cotman CW
    Neuroscience, 2007;144(3):1033-44.
    Species: Mouse
    Sample Types: Serum
  105. Ghrelin promotes thymopoiesis during aging.
    Authors: Dixit VD, Yang H, Sun Y, Weeraratna AT, Youm YH, Smith RG, Taub DD
    J. Clin. Invest., 2007;117(10):2778-90.
    Species: Mouse
    Sample Types: Serum
  106. Microcytosis in ank/ank mice and the role of ANKH in promoting erythroid differentiation.
    Authors: Wang J, Wang C, Tsui HW, Las Heras F, Cheng EY, Iscove NN, Chiu B, Inman RD, Pritzker KP, Tsui FW
    Exp. Cell Res., 2007;313(20):4120-9.
    Species: Mouse
    Sample Types: Serum
  107. Peripheral elevation of IGF-1 fails to alter Abeta clearance in multiple in vivo models.
    Authors: Lanz TA, Salatto CT, Semproni AR, Marconi M, Brown TM, Richter KE, Schmidt K, Nelson FR, Schachter JB
    Biochem. Pharmacol., 2007;75(5):1093-103.
    Species: Rat
    Sample Types: Plasma

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Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit
By Anonymous on 11/17/2020
Application: Sample Tested: EDTA Plasma

The performance of this analysis was great with samples in the log part of the standard curve.


Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit
By Anonymous on 04/10/2019
Application: Sample Tested: EDTA Plasma

We routinely use this ELISA to quantify mouse IGF-1 levels. We have published data from this ELISA. The data shown here confirms previously published data suggesting that IGFBP-1-KO causes increase in IGF-1 levels


Mouse/Rat IGF-I Quantikine ELISA Kit
By Anonymous on 04/01/2018
Application: Sample Tested: Serum

Mouse/Rat IGF-I Quantikine ELISA Kit
By Anonymous on 11/08/2016
Application: Sample Tested: Cell Culture Supernates