Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Inositol Monophosphatase 3/IMPAD1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Inositol Monophosphatase 3/IMPAD1
Asn145-His356
Accession # Q80V26

Specificity

 Detects recombinant mouse Inositol Monophosphatase 3/IMPAD1 in direct ELISAs and Western blots. Detects mouse Inositol Monophosphatase 3/IMPAD1 and rat Inositol Monophosphatase 3/IMPAD1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody

Detection of Mouse and Rat Inositol Monophosphatase 3/IMPAD1 antibody by Western Blot.

Detection of Mouse and Rat Inositol Monophosphatase 3/IMPAD1 by Western Blot.

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Inositol Monophosphatase 3/IMPAD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7028) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Inositol Monophosphatase 3/IMPAD1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Inositol Monophosphatase 3/IMPAD1 antibody in Mouse Mesenchymal Stem Cells by Immunocytochemistry (ICC).

Inositol Monophosphatase 3/IMPAD1 in Mouse Mesenchymal Stem Cells.

Inositol Monophosphatase 3/IMPAD1 was detected in immersion fixed mouse mesenchymal stem cells differentiated into chondrocytes using Sheep Anti-Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7028) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse mesenchymal stem cells differentiated into chondrocytes

Western Blot

1 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line and PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Inositol Monophosphatase 3/IMPAD1

IMPAD1 (Inositol monophosphatase domain-containing protein 1; also IMPA3, gPAPP and IMPase 3) is a 40-42 kDa member of the inositol monophosphatase family of proteins. It is expressed in embryo, and found in Purkinje cells, brain stem, lung and chondrocytes. IMPAD1 in theory may catalyze the synthesis of myo-inositol from myo-inositol monophosphate. Free myo-inositol is used to generate inositol phospholipid, an essential component of intracellular signaling pathways that mobilize calcium. IMPAD1 is reported to promote sulfation of chondroitin by converting PAP (an endproduct of the sulfation process) to 5'-AMP. PAP is a known inhibitor of SULTs. IMPAD1 is a 356 amino acid (aa) type II transmembrane Golgi-embedded glycoprotein. It contains a short cytoplasmic tail (aa 1-12) and an extended luminal region (aa 34-356) that contains its catalytic domain (aa 60-347). Over aa 145-356, mouse IMPAD1 shares 99% and 93% aa sequence identity with rat and human IMPAD1, respectively.

Alternate Names

IMP3, IMPA3, IMPase 3

Entrez Gene IDs

54928 (Human); 242291 (Mouse); 312952 (Rat)

Gene Symbol

BPNT2

UniProt

Q80V26

Additional Inositol Monophosphatase 3/IMPAD1 Products

Product Documents for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody

For research use only

Citations for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody

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Protocols

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