Mouse/Rat Integrin  beta 5 Antibody

Catalog # Availability Size / Price Qty
AF8035
AF8035-SP
Detection of Mouse and Rat Integrin  beta 5 by Western Blot.
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Mouse/Rat Integrin  beta 5 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 5
Gly24-Asn719
Accession # NP_001139356
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat Integrin  beta 5 by Western Blot. View Larger

Detection of Mouse and Rat Integrin  beta 5 by Western Blot. Western blot shows lysates of CMT-93 mouse rectal carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Integrin  beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin  beta 5 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Integrin  beta 5 in bEnd.3 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Integrin  beta 5 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Sheep Anti-Mouse/Rat Integrin  beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

Immunohistochemistry Integrin  beta 5 in Mouse Brain. View Larger

Integrin  beta 5 in Mouse Brain. Integrin  beta 5 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse/Rat Integrin  beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010). Specific staining was localized to glial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 5

The beta 5 integrin is a 95-110 kDa member of the integrin beta chain family, integrin superfamily of molecules. It is expressed by select cell types, including microglia, keratinocytes, fibroblasts, endothelial cells, adhesive monocytes and macrophages, retinal pigment epithelium, immature dendritic cells and colonic epithelium. The Integrin beta 5 subunit forms a noncovalent heterodimer with the alpha v integrin subunit, and together they bind vitronectin, and the Thy-1 antigen. alpha v beta 5 is reported to participate in phagocytosis, and act in concert with CD81, CD36 and stabilin-2 on the cell surface. When alpha v beta 5 binds Thy-1, the heterodimer apparently interfers with cell membrane activation of TGF-beta. Intracellularly, it appears to regulate actin skeleton remodeling via focal adhesion complex formation, and may contribute to cryptic pathways associated with antigen presentation. Mature mouse Integrin beta 5 is a 775 amino acid (aa) type I transmembrane glycoprotein that contains a 696 aa extracellular domain (ECD) (aa 24-719) and a 56 aa cytoplasmic region. The ECD contains a 243 aa vWF-A domain (aa 136-378) and a Cys-rich tandem repeat region (aa 465-630). There is one splice variant that shows a 57 aa substitution for aa 760-798. This splice form does not appear to be expressed by the human Integrin beta 5 gene. Over aa 24-719, mouse Integrin beta 5 shares 91% and 97% aa sequence identity with human and rat Integrin beta 5, respectively.

Entrez Gene IDs
3693 (Human); 16419 (Mouse); 257645 (Rat)
Alternate Names
FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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