Mouse/Rat Integrin  beta 5 Antibody

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Detection of Mouse and Rat Integrin  beta 5 by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Mouse/Rat Integrin  beta 5 Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 5
Accession # NP_001139356
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
5-15 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat Integrin  beta 5 antibody by Western Blot. View Larger

Detection of Mouse and Rat Integrin beta 5 by Western Blot. Western blot shows lysates of CMT-93 mouse rectal carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin beta 5 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Integrin  beta 5 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 5 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Sheep Anti-Mouse/Rat Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

Immunohistochemistry Integrin  beta 5 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

Integrin beta 5 in Mouse Brain. Integrin beta 5 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse/Rat Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8035) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010). Specific staining was localized to glial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 5

The beta 5 integrin is a 95-110 kDa member of the integrin beta chain family, integrin superfamily of molecules. It is expressed by select cell types, including microglia, keratinocytes, fibroblasts, endothelial cells, adhesive monocytes and macrophages, retinal pigment epithelium, immature dendritic cells and colonic epithelium. The Integrin beta 5 subunit forms a noncovalent heterodimer with the alpha v integrin subunit, and together they bind vitronectin, and the Thy-1 antigen. alpha v beta 5 is reported to participate in phagocytosis, and act in concert with CD81, CD36 and stabilin-2 on the cell surface. When alpha v beta 5 binds Thy-1, the heterodimer apparently interfers with cell membrane activation of TGF-beta. Intracellularly, it appears to regulate actin skeleton remodeling via focal adhesion complex formation, and may contribute to cryptic pathways associated with antigen presentation. Mature mouse Integrin beta 5 is a 775 amino acid (aa) type I transmembrane glycoprotein that contains a 696 aa extracellular domain (ECD) (aa 24-719) and a 56 aa cytoplasmic region. The ECD contains a 243 aa vWF-A domain (aa 136-378) and a Cys-rich tandem repeat region (aa 465-630). There is one splice variant that shows a 57 aa substitution for aa 760-798. This splice form does not appear to be expressed by the human Integrin beta 5 gene. Over aa 24-719, mouse Integrin beta 5 shares 91% and 97% aa sequence identity with human and rat Integrin beta 5, respectively.

Entrez Gene IDs
3693 (Human); 16419 (Mouse); 257645 (Rat); 102123619 (Cynomolgus Monkey)
Alternate Names
FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5

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Citation for Mouse/Rat Integrin  beta 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The Angiogenesis Inhibitor Isthmin-1 (ISM1) Is Overexpressed in Experimental Models of Glomerulopathy and Impairs the Viability of Podocytes
    Authors: V Sahiri, J Caron, E Roger, C Desterke, K Ghachem, I Mohamadou, J Serre, N Prakoura, S Fellahi, S Placier, S Adriouch, L Zhang, CE Chadjichri, C Chatzianto, HK Lorenzo, JJ Boffa
    International Journal of Molecular Sciences, 2023-02-01;24(3):.
    Species: Human, Rat
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Immunoprecipitation


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