Mouse/Rat MMP-2 Antibody

AF1488 21 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1488
AF1488-SP

Bulk Orders

Mouse/Rat MMP-2 Antibody in Immunohistochemistry

1 Image

Product Details

Citations (21)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat MMP-2 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat MMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human MMP-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) MMP-3 and rmMMP-9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP-2
Ile34-Cys662
Accession # P33434

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse/Rat MMP‑2 (Catalog # 924-MP)

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse/Rat MMP‑2 (Catalog # 924‑MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

View Larger

MMP‑2 in Mouse Thymus. MMP-2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse/ Rat MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites. The amino acid sequences of the proenzymes are identical between mouse and rat.

Long Name

Matrix Metalloproteinase 2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Alternate Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Rat MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
Filter your results:

Filter by:

ATRQ Î²-001 Vaccine Prevents Experimental Abdominal Aortic Aneurysms
Authors: H Zhang, M Liao, M Cao, Z Qiu, X Yan, Y Zhou, H Wu, Y Wang, J Zheng, J Ding, M Wang, Y Liao, X Chen
J Am Heart Assoc, 2019;8(18):e012341.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells
Authors: LQ Liu, HS Li, SP Nie, MY Shen, JL Hu, MY Xie
Int J Mol Sci, 2018;19(2):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Chemopreventive Potential of 2,3,5,4'-Tetrahydroxystilbene-2-O-?-D-glucoside on the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colorectal Cancer in Rats
Authors: CL Lin, JH Jeng, CC Wu, SL Hsieh, GC Huang, W Leung, CT Lee, CY Chen, CH Lee
Biomed Res Int, 2017;2017(0):3634915.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression
Authors: PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang
J Hematol Oncol, 2017;10(1):33.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
Physiol Rep, 2016;4(23):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury.
Authors: Dohi, Kenji, Kraemer, Brian C, Erickson, Michelle, McMillan, Pamela J, Kovac, Andrej, Flachbartova, Zuzana, Hansen, Kim M, Shah, Gul N, Sheibani, Nader, Salameh, Therese, Banks, William
PLoS ONE, 2014;9(9):e108034.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Deletion of the EphA2 receptor exacerbates myocardial injury and the progression of ischemic cardiomyopathy.
Authors: O'Neal W, Griffin W, Kent S, Faiz F, Hodges J, Vuncannon J, Virag J
Front Physiol, 2014;5(0):132.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
PLoS ONE, 2014;9(5):e96286.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
J Immunol, 2013;190(10):5329-36.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)
KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
Authors: Salmon M, Johnston W, Woo A, Pope N, Su G, Upchurch G, Owens G, Ailawadi G
Circulation, 2013;128(11):S163-74.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
Kidney Int, 2012;82(12):1261-70.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Site-Specific Expression of Gelatinolytic Activity during Morphogenesis of the Secondary Palate in the Mouse Embryo.
Authors: Gkantidis, Nikolaos, Blumer, Susan, Katsaros, Christos, Graf, Daniel, Chiquet, Matthias
PLoS ONE, 2012;7(10):e47762.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
J. Biol. Chem., 2012;287(20):16356-64.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C
Diabetologia, 2011;54(6):1468-79.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
Authors: Pai A, Leaf EM, El-Abbadi M, Giachelli CM
Am. J. Pathol., 2011;178(2):764-73.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats.
Authors: Lind T, Lind PM, Jacobson A, Hu L, Sundqvist A, Risteli J, Yebra-Rodriguez A, Rodriguez-Navarro A, Andersson G, Melhus H
Bone, 2011;48(3):496-506.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
Authors: Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA
Kidney Int., 2008;74(1):47-51.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Interleukin-1 receptor type I signaling critically regulates infarct healing and cardiac remodeling.
Authors: Bujak M, Dobaczewski M, Chatila K, Mendoza LH, Li N, Reddy A, Frangogiannis NG
Am. J. Pathol., 2008;173(1):57-67.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
Blood, 2007;109(5):2234-42.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Characterization of lymphangiogenesis in a model of adult skin regeneration.
Authors: Rutkowski JM, Boardman KC, Swartz MA
Am. J. Physiol. Heart Circ. Physiol., 2006;291(3):H1402-10.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
Authors: Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP
Infect. Immun., 2005;73(10):6962-73.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot