Mouse/Rat MMP-2 Antibody

Catalog # Availability Size / Price Qty
AF1488
AF1488-SP
MMP‑2 in Mouse Thymus.
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Product Details
Citations (20)
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Mouse/Rat MMP-2 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat MMP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human MMP-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-7, rmMMP-8, rmMMP-9, rmMMP‑12, and recombinant rat MMP-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP-2
Ile34-Cys662
Accession # P33434
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse/Rat MMP‑2 (Catalog # 924-MP)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse/Rat MMP‑2 (Catalog # 924‑MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry MMP‑2 in Mouse Thymus. View Larger

MMP‑2 in Mouse Thymus.

MMP‑2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse/
Rat MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites. The amino acid sequences of the proenzymes are identical between mouse and rat.  

Long Name
Matrix Metalloproteinase 2
Entrez Gene IDs
4313 (Human); 17390 (Mouse)
Alternate Names
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Product Datasheets

Citations for Mouse/Rat MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells
    Authors: LQ Liu, HS Li, SP Nie, MY Shen, JL Hu, MY Xie
    Int J Mol Sci, 2018;19(2):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  2. Chemopreventive Potential of 2,3,5,4'-Tetrahydroxystilbene-2-O-?-D-glucoside on the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colorectal Cancer in Rats
    Authors: CL Lin, JH Jeng, CC Wu, SL Hsieh, GC Huang, W Leung, CT Lee, CY Chen, CH Lee
    Biomed Res Int, 2017;2017(0):3634915.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  3. Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression
    Authors: PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang
    J Hematol Oncol, 2017;10(1):33.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  4. Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
    Physiol Rep, 2016;4(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Deletion of the EphA2 receptor exacerbates myocardial injury and the progression of ischemic cardiomyopathy.
    Authors: O'Neal W, Griffin W, Kent S, Faiz F, Hodges J, Vuncannon J, Virag J
    Front Physiol, 2014;5(0):132.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  6. The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
    Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
    PLoS ONE, 2014;9(5):e96286.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  7. Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury.
    Authors: Dohi, Kenji, Kraemer, Brian C, Erickson, Michelle, McMillan, Pamela J, Kovac, Andrej, Flachbartova, Zuzana, Hansen, Kim M, Shah, Gul N, Sheibani, Nader, Salameh, Therese, Banks, William
    PLoS ONE, 2014;9(9):e108034.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  8. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  9. KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
    Authors: Salmon M, Johnston W, Woo A, Pope N, Su G, Upchurch G, Owens G, Ailawadi G
    Circulation, 2013;128(11):S163-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  10. Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
    Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
    J. Biol. Chem., 2012;287(20):16356-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  11. Site-Specific Expression of Gelatinolytic Activity during Morphogenesis of the Secondary Palate in the Mouse Embryo.
    Authors: Gkantidis, Nikolaos, Blumer, Susan, Katsaros, Christos, Graf, Daniel, Chiquet, Matthias
    PLoS ONE, 2012;7(10):e47762.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  12. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012;82(12):1261-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  13. Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
    Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C
    Diabetologia, 2011;54(6):1468-79.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  14. Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
    Authors: Pai A, Leaf EM, El-Abbadi M, Giachelli CM
    Am. J. Pathol., 2011;178(2):764-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  15. High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats.
    Authors: Lind T, Lind PM, Jacobson A, Hu L, Sundqvist A, Risteli J, Yebra-Rodriguez A, Rodriguez-Navarro A, Andersson G, Melhus H
    Bone, 2011;48(3):496-506.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  16. Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
    Authors: Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA
    Kidney Int., 2008;74(1):47-51.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  17. Interleukin-1 receptor type I signaling critically regulates infarct healing and cardiac remodeling.
    Authors: Bujak M, Dobaczewski M, Chatila K, Mendoza LH, Li N, Reddy A, Frangogiannis NG
    Am. J. Pathol., 2008;173(1):57-67.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  18. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2007;109(5):2234-42.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  19. Characterization of lymphangiogenesis in a model of adult skin regeneration.
    Authors: Rutkowski JM, Boardman KC, Swartz MA
    Am. J. Physiol. Heart Circ. Physiol., 2006;291(3):H1402-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
    Authors: Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP
    Infect. Immun., 2005;73(10):6962-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

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