Mouse/Rat MMP-2 Antibody

Catalog # Availability Size / Price Qty
MMP‑2 in Mouse Thymus.
1 Image
Product Details
Citations (23)
Supplemental Products

Mouse/Rat MMP-2 Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat MMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human MMP-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) MMP-3 and rmMMP-9 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse MMP-2
Accession # P33434
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse/Rat MMP‑2 (Catalog # 924-MP)
5-15 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse/Rat MMP‑2 (Catalog # 924‑MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry MMP-2 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

MMP‑2 in Mouse Thymus. MMP-2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse/ Rat MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites. The amino acid sequences of the proenzymes are identical between mouse and rat.  

Long Name
Matrix Metalloproteinase 2
Entrez Gene IDs
4313 (Human); 17390 (Mouse)
Alternate Names
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Product Datasheets

Citations for Mouse/Rat MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Nicotine Exacerbates TAAD Formation Induced by Smooth Muscle-Specific Deletion of the TGF-beta Receptor 2
    Authors: C Chun, X Qi, F Wang, KB Madrid, LA Saldarriag, MR Fisch, ML Brantly, GR Upchurch, Z Jiang
    Journal of Immunology Research, 2021;2021(0):6880036.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
    Authors: SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh
    Exp. Mol. Med., 2020;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. ATRQ β-001 Vaccine Prevents Experimental Abdominal Aortic Aneurysms
    Authors: H Zhang, M Liao, M Cao, Z Qiu, X Yan, Y Zhou, H Wu, Y Wang, J Zheng, J Ding, M Wang, Y Liao, X Chen
    J Am Heart Assoc, 2019;8(18):e012341.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells
    Authors: LQ Liu, HS Li, SP Nie, MY Shen, JL Hu, MY Xie
    Int J Mol Sci, 2018;19(2):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression
    Authors: PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang
    J Hematol Oncol, 2017;10(1):33.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Chemopreventive Potential of 2,3,5,4'-Tetrahydroxystilbene-2-O-?-D-glucoside on the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colorectal Cancer in Rats
    Authors: CL Lin, JH Jeng, CC Wu, SL Hsieh, GC Huang, W Leung, CT Lee, CY Chen, CH Lee
    Biomed Res Int, 2017;2017(0):3634915.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
    Physiol Rep, 2016;4(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury.
    Authors: Dohi, Kenji, Kraemer, Brian C, Erickson, Michelle, McMillan, Pamela J, Kovac, Andrej, Flachbartova, Zuzana, Hansen, Kim M, Shah, Gul N, Sheibani, Nader, Salameh, Therese, Banks, William
    PLoS ONE, 2014;9(9):e108034.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
    Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
    PLoS ONE, 2014;9(5):e96286.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Deletion of the EphA2 receptor exacerbates myocardial injury and the progression of ischemic cardiomyopathy.
    Authors: O'Neal W, Griffin W, Kent S, Faiz F, Hodges J, Vuncannon J, Virag J
    Front Physiol, 2014;5(0):132.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  12. KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
    Authors: Salmon M, Johnston W, Woo A, Pope N, Su G, Upchurch G, Owens G, Ailawadi G
    Circulation, 2013;128(11):S163-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012;82(12):1261-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Site-Specific Expression of Gelatinolytic Activity during Morphogenesis of the Secondary Palate in the Mouse Embryo.
    Authors: Gkantidis, Nikolaos, Blumer, Susan, Katsaros, Christos, Graf, Daniel, Chiquet, Matthias
    PLoS ONE, 2012;7(10):e47762.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
    Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
    J. Biol. Chem., 2012;287(20):16356-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats.
    Authors: Lind T, Lind PM, Jacobson A, Hu L, Sundqvist A, Risteli J, Yebra-Rodriguez A, Rodriguez-Navarro A, Andersson G, Melhus H
    Bone, 2011;48(3):496-506.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  17. Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
    Authors: Pai A, Leaf EM, El-Abbadi M, Giachelli CM
    Am. J. Pathol., 2011;178(2):764-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
    Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C
    Diabetologia, 2011;54(6):1468-79.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
    Authors: Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA
    Kidney Int., 2008;74(1):47-51.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. Interleukin-1 receptor type I signaling critically regulates infarct healing and cardiac remodeling.
    Authors: Bujak M, Dobaczewski M, Chatila K, Mendoza LH, Li N, Reddy A, Frangogiannis NG
    Am. J. Pathol., 2008;173(1):57-67.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2007;109(5):2234-42.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Characterization of lymphangiogenesis in a model of adult skin regeneration.
    Authors: Rutkowski JM, Boardman KC, Swartz MA
    Am. J. Physiol. Heart Circ. Physiol., 2006;291(3):H1402-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  23. Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
    Authors: Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP
    Infect. Immun., 2005;73(10):6962-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse/Rat MMP-2 Antibody

There are currently no reviews for this product. Be the first to review Mouse/Rat MMP-2 Antibody and earn rewards!

Have you used Mouse/Rat MMP-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review