Mouse/Rat MMP-2 Antibody

AF1488 20 Citations Datasheet / COA / SDS

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AF1488
AF1488-SP

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Citations (20)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat MMP-2 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat MMP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human MMP-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-7, rmMMP-8, rmMMP-9, rmMMP‑12, and recombinant rat MMP-8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP-2
Ile34-Cys662
Accession # P33434

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse/Rat MMP‑2 (Catalog # 924-MP)

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse/Rat MMP‑2 (Catalog # 924‑MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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MMP‑2 in Mouse Thymus.

MMP‑2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse/
Rat MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites. The amino acid sequences of the proenzymes are identical between mouse and rat.

Long Name

Matrix Metalloproteinase 2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Alternate Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Rat MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells
Authors: LQ Liu, HS Li, SP Nie, MY Shen, JL Hu, MY Xie
Int J Mol Sci, 2018;19(2):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB
Chemopreventive Potential of 2,3,5,4'-Tetrahydroxystilbene-2-O-?-D-glucoside on the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colorectal Cancer in Rats
Authors: CL Lin, JH Jeng, CC Wu, SL Hsieh, GC Huang, W Leung, CT Lee, CY Chen, CH Lee
Biomed Res Int, 2017;2017(0):3634915.
Species: Rat
Sample Types: Tissue Lysates
Applications: Western Blot
Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression
Authors: PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang
J Hematol Oncol, 2017;10(1):33.
Species: Human
Sample Types: Whole Tissue
Applications: IHC - Paraffin embedded
Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
Physiol Rep, 2016;4(23):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Deletion of the EphA2 receptor exacerbates myocardial injury and the progression of ischemic cardiomyopathy.
Authors: O'Neal W, Griffin W, Kent S, Faiz F, Hodges J, Vuncannon J, Virag J
Front Physiol, 2014;5(0):132.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB
The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
PLoS ONE, 2014;9(5):e96286.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC - Paraffin embedded
Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury.
Authors: Dohi, Kenji, Kraemer, Brian C, Erickson, Michelle, McMillan, Pamela J, Kovac, Andrej, Flachbartova, Zuzana, Hansen, Kim M, Shah, Gul N, Sheibani, Nader, Salameh, Therese, Banks, William
PLoS ONE, 2014;9(9):e108034.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
J Immunol, 2013;190(10):5329-36.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture
KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
Authors: Salmon M, Johnston W, Woo A, Pope N, Su G, Upchurch G, Owens G, Ailawadi G
Circulation, 2013;128(11):S163-74.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded
Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
J. Biol. Chem., 2012;287(20):16356-64.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded

Site-Specific Expression of Gelatinolytic Activity during Morphogenesis of the Secondary Palate in the Mouse Embryo.
Authors: Gkantidis, Nikolaos, Blumer, Susan, Katsaros, Christos, Graf, Daniel, Chiquet, Matthias
PLoS ONE, 2012;7(10):e47762.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen

Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
Kidney Int, 2012;82(12):1261-70.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC - Not specified

Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C
Diabetologia, 2011;54(6):1468-79.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB

Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
Authors: Pai A, Leaf EM, El-Abbadi M, Giachelli CM
Am. J. Pathol., 2011;178(2):764-73.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded

High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats.
Authors: Lind T, Lind PM, Jacobson A, Hu L, Sundqvist A, Risteli J, Yebra-Rodriguez A, Rodriguez-Navarro A, Andersson G, Melhus H
Bone, 2011;48(3):496-506.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
Authors: Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA
Kidney Int., 2008;74(1):47-51.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB

Interleukin-1 receptor type I signaling critically regulates infarct healing and cardiac remodeling.
Authors: Bujak M, Dobaczewski M, Chatila K, Mendoza LH, Li N, Reddy A, Frangogiannis NG
Am. J. Pathol., 2008;173(1):57-67.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB

A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
Blood, 2007;109(5):2234-42.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB

Characterization of lymphangiogenesis in a model of adult skin regeneration.
Authors: Rutkowski JM, Boardman KC, Swartz MA
Am. J. Physiol. Heart Circ. Physiol., 2006;291(3):H1402-10.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
Authors: Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP
Infect. Immun., 2005;73(10):6962-73.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB